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Literature summary extracted from

  • Hwang, S.K.; Hamada, S.; Okita, T.W.
    ATP binding site in the plant ADP-glucose pyrophosphorylase large subunit (2006), FEBS Lett., 580, 6741-6748.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.7.27 3-phosphoglycerate 50% activation at 0.12 mM for wild-type, at 1.38 mM for mutant D157E, at 0.03 mM for mutant K41R of large subunit plus D143N of small subunit Solanum tuberosum

Protein Variants

EC Number Protein Variants Comment Organism
2.7.7.27 A132D mutation in ATP binding region of large subunit, kinetic analysis Solanum tuberosum
2.7.7.27 A132F mutation in ATP binding region of large subunit, kinetic analysis Solanum tuberosum
2.7.7.27 A132N mutation in ATP binding region of large subunit, kinetic analysis Solanum tuberosum
2.7.7.27 A132V mutation in ATP binding region of large subunit, kinetic analysis Solanum tuberosum
2.7.7.27 D157E mutation in ATP binding region of large subunit, kinetic analysis Solanum tuberosum
2.7.7.27 D157L mutation in ATP binding region of large subunit, kinetic analysis Solanum tuberosum
2.7.7.27 D157L mutation in ATP binding region of large subunit, plus mutation D143N in small subunit, kinetic analysis Solanum tuberosum
2.7.7.27 D157N mutation in ATP binding region of large subunit, kinetic analysis Solanum tuberosum
2.7.7.27 G128A mutation in ATP binding region of large subunit, kinetic analysis Solanum tuberosum
2.7.7.27 G128L mutation in ATP binding region of large subunit, kinetic analysis Solanum tuberosum
2.7.7.27 G267L mutation in ATP binding region of large subunit, kinetic analysis Solanum tuberosum
2.7.7.27 G267S mutation in ATP binding region of large subunit, kinetic analysis Solanum tuberosum
2.7.7.27 G36A mutation in ATP binding region of large subunit, kinetic analysis Solanum tuberosum
2.7.7.27 G37A mutation in ATP binding region of large subunit, kinetic analysis Solanum tuberosum
2.7.7.27 K41R mutation in ATP binding region of large subunit plus mutation D143N in small subunit, kinetic analysis Solanum tuberosum
2.7.7.27 K41R mutation in ATP binding region of large subunit, kinetic analysis Solanum tuberosum
2.7.7.27 K41R/T51K mutation in ATP binding region of large subunit, kinetic analysis Solanum tuberosum
2.7.7.27 K41R/T51K mutation in ATP binding region of large subunit, plus mutation D143N in small subunit, kinetic analysis Solanum tuberosum
2.7.7.27 Q127M mutation in ATP binding region of large subunit, kinetic analysis Solanum tuberosum
2.7.7.27 T129V mutation in ATP binding region of large subunit, kinetic analysis Solanum tuberosum
2.7.7.27 T129V/A132V mutation in ATP binding region of large subunit, kinetic analysis Solanum tuberosum
2.7.7.27 T51K mutation in ATP binding region of large subunit, kinetic analysis Solanum tuberosum
2.7.7.27 T51K mutation in ATP binding region of large subunit, plus mutation D143N in small subunit, kinetic analysis Solanum tuberosum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.7.27 0.1
-
 alpha-D-glucose 1-phosphate wild-type Solanum tuberosum
2.7.7.27 0.12
-
 alpha-D-glucose 1-phosphate mutant D157N Solanum tuberosum
2.7.7.27 0.15
-
 alpha-D-glucose 1-phosphate mutant D157E Solanum tuberosum
2.7.7.27 0.16
-
 alpha-D-glucose 1-phosphate mutant G267S Solanum tuberosum
2.7.7.27 0.16
-
 alpha-D-glucose 1-phosphate mutant Q127M Solanum tuberosum
2.7.7.27 0.17
-
 ATP wild-type Solanum tuberosum
2.7.7.27 0.17
-
 alpha-D-glucose 1-phosphate mutation K41R/T51K, large subunit, plus D143N, small subunit Solanum tuberosum
2.7.7.27 0.18
-
 ATP mutant Q127M Solanum tuberosum
2.7.7.27 0.19
-
 ATP mutant K41R Solanum tuberosum
2.7.7.27 0.21
-
 ATP mutant G267S Solanum tuberosum
2.7.7.27 0.22
-
 alpha-D-glucose 1-phosphate mutant D157L Solanum tuberosum
2.7.7.27 0.22
-
 alpha-D-glucose 1-phosphate mutant K41R/T51K Solanum tuberosum
2.7.7.27 0.24
-
 ATP mutant G37A Solanum tuberosum
2.7.7.27 0.24
-
 alpha-D-glucose 1-phosphate mutant K41R Solanum tuberosum
2.7.7.27 0.24
-
 ATP mutant T51K Solanum tuberosum
2.7.7.27 0.25
-
 alpha-D-glucose 1-phosphate mutant G37A Solanum tuberosum
2.7.7.27 0.26
-
 ATP mutant G36A Solanum tuberosum
2.7.7.27 0.27
-
 ATP mutant K41R/T51K Solanum tuberosum
2.7.7.27 0.3
-
 ATP mutant T129V Solanum tuberosum
2.7.7.27 0.31
-
 ATP mutant D143N, small subunit Solanum tuberosum
2.7.7.27 0.32
-
 ATP mutant D157E Solanum tuberosum
2.7.7.27 0.32
-
 alpha-D-glucose 1-phosphate mutant T51K Solanum tuberosum
2.7.7.27 0.35
-
 ATP mutant A132F Solanum tuberosum
2.7.7.27 0.35
-
 ATP mutation K41R/T51K, large subunit, plus D143N, small subunit Solanum tuberosum
2.7.7.27 0.36
-
 ATP mutant A132V Solanum tuberosum
2.7.7.27 0.36
-
 ATP mutant G128A Solanum tuberosum
2.7.7.27 0.38
-
 alpha-D-glucose 1-phosphate mutation K41R, large subunit, plus D143N, small subunit Solanum tuberosum
2.7.7.27 0.39
-
 ATP mutant A132D Solanum tuberosum
2.7.7.27 0.39
-
 ATP mutant A132N Solanum tuberosum
2.7.7.27 0.4
-
 ATP mutant D157L Solanum tuberosum
2.7.7.27 0.4
-
 alpha-D-glucose 1-phosphate mutant G36A Solanum tuberosum
2.7.7.27 0.4
-
 ATP mutation K41R, large subunit, plus D143N, small subunit Solanum tuberosum
2.7.7.27 0.43
-
 ATP mutant T129V/A132V Solanum tuberosum
2.7.7.27 0.43
-
 ATP mutation T51K, large subunit, plus D143N, small subunit Solanum tuberosum
2.7.7.27 0.44
-
 alpha-D-glucose 1-phosphate mutant G128A Solanum tuberosum
2.7.7.27 0.46
-
 ATP mutant G128L Solanum tuberosum
2.7.7.27 0.47
-
 alpha-D-glucose 1-phosphate mutant G267L Solanum tuberosum
2.7.7.27 0.54
-
 alpha-D-glucose 1-phosphate mutant T129V Solanum tuberosum
2.7.7.27 0.57
-
 ATP mutant G267L Solanum tuberosum
2.7.7.27 0.58
-
 ATP mutant D157N Solanum tuberosum
2.7.7.27 0.63
-
 alpha-D-glucose 1-phosphate mutant A132N Solanum tuberosum
2.7.7.27 0.64
-
 alpha-D-glucose 1-phosphate mutant A132D Solanum tuberosum
2.7.7.27 0.71
-
 alpha-D-glucose 1-phosphate mutant G128L Solanum tuberosum
2.7.7.27 0.81
-
 alpha-D-glucose 1-phosphate mutant A132V Solanum tuberosum
2.7.7.27 0.88
-
 alpha-D-glucose 1-phosphate mutant T129V/A132V Solanum tuberosum
2.7.7.27 0.97
-
 alpha-D-glucose 1-phosphate mutant A132F Solanum tuberosum
2.7.7.27 1.01
-
 alpha-D-glucose 1-phosphate mutation T51K, large subunit, plus D143N, small subunit Solanum tuberosum

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.27 Solanum tuberosum P23509
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.7.27 tuber
-
 Solanum tuberosum
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.7.27 0.01
-
 mutant D143N, small subunit Solanum tuberosum
2.7.7.27 0.02
-
 mutation K41R, large subunit, plus D143N, small subunit Solanum tuberosum
2.7.7.27 1.02
-
 mutation T51K, large subunit, plus D143N, small subunit Solanum tuberosum
2.7.7.27 1.6
-
 mutant T129V/A132V Solanum tuberosum
2.7.7.27 2.9
-
 mutation K41R/T51K, large subunit, plus D143N, small subunit Solanum tuberosum
2.7.7.27 3.2
-
 mutant G128L Solanum tuberosum
2.7.7.27 5.2
-
 mutant A132F Solanum tuberosum
2.7.7.27 5.2
-
 mutant D157L Solanum tuberosum
2.7.7.27 5.4
-
 mutant A132V Solanum tuberosum
2.7.7.27 5.6
-
 mutant A132D Solanum tuberosum
2.7.7.27 6.5
-
 mutant G267L Solanum tuberosum
2.7.7.27 6.7
-
 mutant A132N Solanum tuberosum
2.7.7.27 7.6
-
 mutant G128A Solanum tuberosum
2.7.7.27 9.5
-
 mutant G37A Solanum tuberosum
2.7.7.27 13.4
-
 mutant D157E Solanum tuberosum
2.7.7.27 14.2
-
 mutant T129V Solanum tuberosum
2.7.7.27 19.1
-
 mutant D157N Solanum tuberosum
2.7.7.27 23
-
 mutant K41R/T51K Solanum tuberosum
2.7.7.27 23.7
-
 mutant T51K Solanum tuberosum
2.7.7.27 26.8
-
 mutant G267S Solanum tuberosum
2.7.7.27 37.5
-
 mutant K41R Solanum tuberosum
2.7.7.27 39.8
-
 mutant Q127M Solanum tuberosum
2.7.7.27 40.5
-
 mutant G36A Solanum tuberosum
2.7.7.27 42
-
 wild-type Solanum tuberosum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.27 ATP + alpha-D-glucose 1-phosphate
-
 Solanum tuberosum ADP-D-glucose + diphosphate
-
 r
2.7.7.27 diphosphate + ADP
-
 Solanum tuberosum ATP + alpha-D-glucose
-
 r

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.7.7.27 0.002
-
 alpha-D-glucose 1-phosphate mutation D157L, large subunit, plus D143N, small subunit Solanum tuberosum
2.7.7.27 0.003
-
 ATP mutation D157L, large subunit, plus D143N, small subunit Solanum tuberosum
2.7.7.27 0.03
-
 ATP mutant D143N, small subunit Solanum tuberosum
2.7.7.27 0.03
-
 alpha-D-glucose 1-phosphate mutant D143N, small subunit Solanum tuberosum
2.7.7.27 0.07
-
 ATP mutation K41R, large subunit, plus D143N, small subunit Solanum tuberosum
2.7.7.27 0.07
-
 alpha-D-glucose 1-phosphate mutation K41R, large subunit, plus D143N, small subunit Solanum tuberosum
2.7.7.27 4
-
 alpha-D-glucose 1-phosphate mutation T51K, large subunit, plus D143N, small subunit Solanum tuberosum
2.7.7.27 4.1
-
 ATP mutation T51K, large subunit, plus D143N, small subunit Solanum tuberosum
2.7.7.27 8
-
 ATP mutant T129V/A132V Solanum tuberosum
2.7.7.27 9
-
 alpha-D-glucose 1-phosphate mutation K41R/T51K, large subunit, plus D143N, small subunit Solanum tuberosum
2.7.7.27 10
-
 alpha-D-glucose 1-phosphate mutant T129V/A132V Solanum tuberosum
2.7.7.27 11
-
 alpha-D-glucose 1-phosphate mutant G128L Solanum tuberosum
2.7.7.27 11.9
-
 ATP mutation K41R/T51K, large subunit, plus D143N, small subunit Solanum tuberosum
2.7.7.27 12
-
 ATP mutant G128L Solanum tuberosum
2.7.7.27 15
-
 ATP mutant D157L Solanum tuberosum
2.7.7.27 15
-
 alpha-D-glucose 1-phosphate mutant D157L Solanum tuberosum
2.7.7.27 16
-
 alpha-D-glucose 1-phosphate mutant A132D Solanum tuberosum
2.7.7.27 16
-
 ATP mutant A132V Solanum tuberosum
2.7.7.27 18
-
 ATP mutant A132D Solanum tuberosum
2.7.7.27 19
-
 ATP mutant A132F Solanum tuberosum
2.7.7.27 21
-
 alpha-D-glucose 1-phosphate mutant A132N Solanum tuberosum
2.7.7.27 22
-
 ATP mutant A132N Solanum tuberosum
2.7.7.27 23
-
 alpha-D-glucose 1-phosphate mutant A132V Solanum tuberosum
2.7.7.27 23
-
 ATP mutant G267L Solanum tuberosum
2.7.7.27 24
-
 alpha-D-glucose 1-phosphate mutant A132F Solanum tuberosum
2.7.7.27 27
-
 alpha-D-glucose 1-phosphate mutant G267L Solanum tuberosum
2.7.7.27 33
-
 alpha-D-glucose 1-phosphate mutant D157E Solanum tuberosum
2.7.7.27 33
-
 ATP mutant G128A Solanum tuberosum
2.7.7.27 33
-
 alpha-D-glucose 1-phosphate mutant G128A Solanum tuberosum
2.7.7.27 40
-
 alpha-D-glucose 1-phosphate mutant G37A Solanum tuberosum
2.7.7.27 43
-
 ATP mutant D157E Solanum tuberosum
2.7.7.27 43
-
 ATP mutant G37A Solanum tuberosum
2.7.7.27 45
-
 ATP mutant T129V Solanum tuberosum
2.7.7.27 47
-
 alpha-D-glucose 1-phosphate mutant T129V Solanum tuberosum
2.7.7.27 63
-
 alpha-D-glucose 1-phosphate mutant D157N Solanum tuberosum
2.7.7.27 75
-
 ATP mutant D157N Solanum tuberosum
2.7.7.27 81
-
 alpha-D-glucose 1-phosphate mutant K41R/T51K Solanum tuberosum
2.7.7.27 83
-
 ATP mutant K41R/T51K Solanum tuberosum
2.7.7.27 85
-
 ATP mutant T51K Solanum tuberosum
2.7.7.27 93
-
 alpha-D-glucose 1-phosphate mutant T51K Solanum tuberosum
2.7.7.27 98
-
 alpha-D-glucose 1-phosphate mutant G267S Solanum tuberosum
2.7.7.27 107
-
 ATP mutant G267S Solanum tuberosum
2.7.7.27 133
-
 alpha-D-glucose 1-phosphate mutant G36A Solanum tuberosum
2.7.7.27 139
-
 ATP mutant K41R Solanum tuberosum
2.7.7.27 142
-
 ATP mutant G36A Solanum tuberosum
2.7.7.27 143
-
 ATP mutant Q127M Solanum tuberosum
2.7.7.27 145
-
 alpha-D-glucose 1-phosphate mutant K41R Solanum tuberosum
2.7.7.27 154
-
 alpha-D-glucose 1-phosphate mutant Q127M Solanum tuberosum
2.7.7.27 158
-
 alpha-D-glucose 1-phosphate wild-type Solanum tuberosum
2.7.7.27 166
-
 ATP wild-type Solanum tuberosum