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Literature summary extracted from

  • Molla, G.; Sacchi, S.; Bernasconi, M.; Pilone, M.S.; Fukui, K.; Polegioni, L.
    Characterization of human D-amino acid oxidase (2006), FEBS Lett., 580, 2358-2364.
    View publication on PubMed

General Stability

EC Number General Stability Organism
1.4.3.3 good stability to urea denaturation Homo sapiens
1.4.3.3 stable homodimer even in the apoprotein form Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.4.3.3 1.3
-
 D-Ala pH 8.5, 25°C Homo sapiens
1.4.3.3 7.5
-
 D-Ser pH 8.5, 25°C Homo sapiens
1.4.3.3 8.5
-
 D-Pro pH 8.5, 25°C Homo sapiens
1.4.3.3 180
-
 Gly pH 8.5, 25°C Homo sapiens
1.4.3.3 2000
-
 D-Asp pH 8.5, 25°C Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
1.4.3.3 Homo sapiens
-
 recombinant
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.4.3.3
-
 Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.4.3.3 D-Ala + H2O + O2
-
 Homo sapiens pyruvate + NH3 + H2O2
-
 ?
1.4.3.3 D-Asp + H2O + O2
-
 Homo sapiens oxaloacetate + NH3 + H2O2
-
 ?
1.4.3.3 D-Pro + H2O + O2
-
 Homo sapiens 2-oxopentanoate + NH3 + H2O2
-
 ?
1.4.3.3 D-Ser + H2O + O2
-
 Homo sapiens 2-oxo-3-hydroxypropionate + NH3 + H2O2
-
 ?
1.4.3.3 Gly + H2O + O2
-
 Homo sapiens acetate + NH3 + H2O2
-
 ?

Subunits

EC Number Subunits Comment Organism
1.4.3.3 dimer
-
 Homo sapiens

Synonyms

EC Number Synonyms Comment Organism
1.4.3.3 hDAAO
-
 Homo sapiens

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.4.3.3 52
-
 unfolding temperature Homo sapiens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.4.3.3 0.9
-
 Gly pH 8.5, 25°C Homo sapiens
1.4.3.3 3
-
 D-Ser pH 8.5, 25°C Homo sapiens
1.4.3.3 5.2
-
 D-Ala pH 8.5, 25°C Homo sapiens
1.4.3.3 6.7
-
 D-Asp pH 8.5, 25°C Homo sapiens
1.4.3.3 10.2
-
 D-Pro pH 8.5, 25°C Homo sapiens

Cofactor

EC Number Cofactor Comment Organism Structure
1.4.3.3 FAD the enzyme contains one molecule of non-covalently bound FAD per protein monomer, which can be easily isolated from the apoprotein by dialysis in the presence of 1 M KBr Homo sapiens