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Literature summary extracted from

  • Chew, G.H.; Galloway, L.C.; McIntyre, N.R.; Schroder, L.A.; Richards, K.M.; Miller, S.A.; Wright, D.W.; Merkler, D.J.
    Ubiquitin and ubiquitin-derived peptides as substrates for peptidylglycine alpha-amidating monooxygenase (2005), FEBS Lett., 579, 4678-4684.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.17.3 0.018
-
 N-acetyl-Leu-His-Leu-Val-Leu-Arg-Leu-Arg-Gly-Gly 100 mM MES/NaOH, pH 6.0, 30 mM NaCl, 1% (v/v) ethanol, 0.001% (v/v) Triton X-100, 1 microM Cu(NO3)2, 10 mg/l catalase, 6 mM ascorbate, 8 microM N-dansyl-Tyr-Val-Gly Bos taurus
1.14.17.3 0.02
-
 N-acetyl-Gln-Lys-Glu-Ser-Thr-Leu-His-Leu-Val-Leu-Arg-Leu-Arg-Gly-Gly 100 mM MES/NaOH, pH 6.0, 30 mM NaCl, 1% (v/v) ethanol, 0.001% (v/v) Triton X-100, 1 microM Cu(NO3)2, 10 mg/l catalase, 6 mM ascorbate, 8 microM N-dansyl-Tyr-Val-Gly Bos taurus
1.14.17.3 0.024
-
 N-acetyl-Leu-Arg-Leu-Arg-Gly-Gly 100 mM MES/NaOH, pH 6.0, 30 mM NaCl, 1% (v/v) ethanol, 0.001% (v/v) Triton X-100, 1 microM Cu(NO3)2, 10 mg/l catalase, 6 mM ascorbate, 8 microM N-dansyl-Tyr-Val-Gly Bos taurus
1.14.17.3 0.12
-
 N-acetyl-Arg-Gly-Gly 100 mM MES/NaOH, pH 6.0, 30 mM NaCl, 1% (v/v) ethanol, 0.001% (v/v) Triton X-100, 1 microM Cu(NO3)2, 10 mg/l catalase, 6 mM ascorbate, 8 microM N-dansyl-Tyr-Val-Gly Bos taurus
1.14.17.3 0.13
-
 N-acetyl-Leu-Arg-Gly-Gly 100 mM MES/NaOH, pH 6.0, 30 mM NaCl, 1% (v/v) ethanol, 0.001% (v/v) Triton X-100, 1 microM Cu(NO3)2, 10 mg/l catalase, 6 mM ascorbate, 8 microM N-dansyl-Tyr-Val-Gly Bos taurus
1.14.17.3 0.6
-
 ubiquitin 100 mM MES/NaOH, pH 6.0, 30 mM NaCl, 1% (v/v) ethanol, 0.001% (v/v) Triton X-100, 1 microM Cu(NO3)2, 10 mg/l catalase, 6 mM ascorbate, 8 microM N-dansyl-Tyr-Val-Gly Bos taurus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.14.17.3 Cu2+
-
 Bos taurus
1.14.17.3 Zn2+
-
 Bos taurus

Organism

EC Number Organism UniProt Comment Textmining
1.14.17.3 Bos taurus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.17.3
-
 Bos taurus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.14.17.3 cell culture
-
 Bos taurus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.17.3 N-acetyl-Arg-Gly-Gly + ascorbate + O2
-
 Bos taurus N-acetyl-Arg-Gly-hydroxyglycin + dehydroascorbate + H2O
-
 ?
1.14.17.3 N-acetyl-Gln-Lys-Glu-Ser-Thr-Leu-His-Leu-Val-Leu-Arg-Leu-Arg-Gly-Gly + ascorbate + O2
-
 Bos taurus N-acetyl-Leu-Arg-Gly-hydroxyglycin + dehydroascorbate + H2O
-
 ?
1.14.17.3 N-acetyl-Leu-Arg-Gly-Gly + ascorbate + O2
-
 Bos taurus N-acetyl-Leu-Arg-Gly-hydroxyglycin + dehydroascorbate + H2O
-
 ?
1.14.17.3 N-acetyl-Leu-Arg-Leu-Arg-Gly-Gly + ascorbate + O2
-
 Bos taurus N-acetyl-Leu-Arg-Gly-hydroxyglycin + dehydroascorbate + H2O
-
 ?
1.14.17.3 N-acetyl-Leu-His-Leu-Val-Leu-Arg-Leu-Arg-Gly-Gly + ascorbate + O2
-
 Bos taurus N-acetyl-Leu-Arg-Gly-hydroxyglycin + dehydroascorbate + H2O
-
 ?
1.14.17.3 ubiquitin + ascorbate + O2
-
 Bos taurus ? + dehydroascorbate + H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.14.17.3 PAM
-
 Bos taurus
1.14.17.3 peptidylglycine alpha-hydroxylating monooxygenase
-
 Bos taurus

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.17.3 ascorbate
-
 Bos taurus