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Literature summary extracted from
- Gene expression and characterization of two 2-oxoacid:ferredoxin oxidoreductases from Aeropyrum pernix K1 (2005), FEBS Lett., 579, 2319-2322.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 1.2.7.1 | expression in Escherichia coli, enzyme Ape2126/2128 | Aeropyrum pernix |
| 1.2.7.11 | expression in Escherichia coli, enzyme Ape1473/1472 | Aeropyrum pernix |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.2.7.1 | KCl | 1020% inhibition is observed with 50 mM KCl, while with higher concentrations (maximum, 0.6 M), 4665% activation is reached, and further increased concentrations of KCl are inhibitory, enzyme Ape2126/2128 | Aeropyrum pernix |  |
| 1.2.7.11 | KCl | 1020% inhibition is observed with 50 mM KCl, while with higher concentrations (maximum, 0.6 M), 4665% activation is reached, and further increased concentrations of KCl are inhibitory, enzyme Ape1473/1472 | Aeropyrum pernix | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.2.7.1 | 0.24 | - |
pyruvate | pH 8.5, 80°C, enzyme Ape2126/2128 | Aeropyrum pernix | |
| 1.2.7.11 | 0.38 | - |
pyruvate | pH 8.5, 80°C, enzyme Ape1473/1472 | Aeropyrum pernix | |
| 1.2.7.11 | 0.5 | - |
2-oxoglutarate | pH 8.5, 80°C, enzyme Ape1473/1472 | Aeropyrum pernix | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 1.2.7.1 | 34936 | - |
1 * 71639 + 1 * 34936, calculated from sequence, enzyme Ape2126/2128 | Aeropyrum pernix |
| 1.2.7.1 | 35000 | - |
1 * 72000 + 1 * 35000, SDS-PAGE, enzyme Ape2126/2128 | Aeropyrum pernix |
| 1.2.7.1 | 71639 | - |
1 * 71639 + 1 * 34936, calculated from sequence, enzyme Ape2126/2128 | Aeropyrum pernix |
| 1.2.7.1 | 72000 | - |
1 * 72000 + 1 * 35000, SDS-PAGE, enzyme Ape2126/2128 | Aeropyrum pernix |
| 1.2.7.1 | 110000 | - |
gel filtration, enzyme Ape2126/2128 | Aeropyrum pernix |
| 1.2.7.11 | 34000 | - |
1 * 67000 + 1 * 34000, SDS-PAGE, enzyme Ape1473/1472 | Aeropyrum pernix |
| 1.2.7.11 | 34410 | - |
1 * 67405 + 1 * 34410, calculated from sequence, enzyme Ape1473/1472 | Aeropyrum pernix |
| 1.2.7.11 | 67000 | - |
1 * 67000 + 1 * 34000, SDS-PAGE, enzyme Ape1473/1472 | Aeropyrum pernix |
| 1.2.7.11 | 67405 | - |
1 * 67405 + 1 * 34410, calculated from sequence, enzyme Ape1473/1472 | Aeropyrum pernix |
| 1.2.7.11 | 100000 | - |
gel filtration, enzyme Ape1473/1472 | Aeropyrum pernix |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.2.7.11 | additional information | Aeropyrum pernix | Ape1473/1472 operates in the TCA cycle of Aeropyrum pernix | ? | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.2.7.1 | Aeropyrum pernix | Q9YA13 and Q9YA11 | Q9YA13: alpha-subunit, Q9YA11: beta-subunit | - |
| 1.2.7.11 | Aeropyrum pernix | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.2.7.1 | enzyme Ape2126/2128 | Aeropyrum pernix |
| 1.2.7.11 | enzyme Ape1473/1472 | Aeropyrum pernix |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.2.7.1 | 2-oxo-4-methyl-thio-butyrate + CoA + 2 methyl viologen | enzyme Ape2126/2128, 37% of the activity compared to pyruvate | Aeropyrum pernix | ? | - |
? | |
| 1.2.7.1 | 2-oxoadipate + CoA + 2 methyl viologen | enzyme Ape2126/2128, 37% of the activity compared to pyruvate | Aeropyrum pernix | ? | - |
? | |
| 1.2.7.1 | 2-oxobutyrate + CoA + 2 oxidized methyl viologen | enzyme Ape2126/2128, 98% of the activity compared to pyruvate | Aeropyrum pernix | propanoyl-CoA + CO2 + 2 reduced methyl viologen | - |
? | |
| 1.2.7.1 | glyoxylate + CoA + 2 oxidized methyl viologen | enzyme Ape2126/2128, 89% of the activity compared to pyruvate | Aeropyrum pernix | ? | - |
? | |
| 1.2.7.1 | hydroxypyruvate + CoA + 2 methyl viologen | enzyme Ape2126/2128, 38% of the activity compared to pyruvate | Aeropyrum pernix | ? | - |
? | |
| 1.2.7.1 | additional information | activity with 2-oxoglutarate is 6% compared to the activity with pyruvate. No activity with 3-methyl-2-oxovalerate, 4-methyl-2-oxovalerate, 2-oxoisocaproic acid or 2-oxooctanoic acid, enzyme Ape2126/2128, | Aeropyrum pernix | ? | - |
? | |
| 1.2.7.1 | pyruvate + CoA + 2 oxidized methyl viologen | enzyme Ape2126/2128 | Aeropyrum pernix | acetyl-CoA + CO2 + 2 reduced methyl viologen + 2 H+ | - |
? | |
| 1.2.7.11 | 2-oxo-4-methylthiobutyrate + CoA + 2 methyl viologen | enzyme Ape1473/1472, 48% of the activity compared to glyoxylate | Aeropyrum pernix | 3-methylthiopropanoyl-CoA + CO2 + 2 reduced methyl viologen + 2 H+ | - |
? | |
| 1.2.7.11 | 2-oxoadipate + CoA + 2 methyl viologen | enzyme Ape1473/1472, 49% of the activity compared to glyoxylate | Aeropyrum pernix | glutaryl-CoA + CO2 + 2 reduced methyl viologen + 2 H+ | - |
? | |
| 1.2.7.11 | 2-oxobutyrate + CoA + 2 oxidized methyl viologen | enzyme Ape1473/1472, 97% of the activity compared to glyoxylate | Aeropyrum pernix | propanoyl-CoA + CO2 + 2 reduced methyl viologen | - |
? | |
| 1.2.7.11 | 2-oxoglutarate + CoA + 2 oxidized methyl viologen | enzyme Ape1473/1472, 94% of the activity compared to glyoxylate | Aeropyrum pernix | succinyl-CoA + CO2 + 2 reduced methyl viologen + 2 H+ | - |
? | |
| 1.2.7.11 | 4-hydroxyphenylpyruvate + CoA + 2 methyl viologen | enzyme Ape1473/1472, 16% of the activity compared to glyoxylate | Aeropyrum pernix | (4-hydroxyphenyl)acetyl-CoA + CO2 + 2 reduced methyl viologen + 2 H+ | - |
? | |
| 1.2.7.11 | glyoxylate + CoA + 2 oxidized methyl viologen | enzyme Ape1473/1472 | Aeropyrum pernix | formyl-CoA + CO2 + 2 reduced methyl viologen + 2 H+ | - |
? | |
| 1.2.7.11 | hydroxypyruvate + CoA + 2 methyl viologen | enzyme Ape1473/1472, 55% of the activity compared to glyoxylate | Aeropyrum pernix | ? | - |
? | |
| 1.2.7.11 | additional information | Ape1473/1472 operates in the TCA cycle of Aeropyrum pernix | Aeropyrum pernix | ? | - |
? | |
| 1.2.7.11 | additional information | no activity with 3-methyl-2-oxovalerate, 4-methyl-2-oxovalerate, 2-oxoisocaproic acid or 2-oxooctanoic acid, enzyme Ape1473/1472 | Aeropyrum pernix | ? | - |
? | |
| 1.2.7.11 | phenylpyruvate + CoA + 2 methyl viologen | enzyme Ape1473/1472, 24% of the activity compared to glyoxylate | Aeropyrum pernix | phenylacetyl-CoA + CO2 + 2 reduced methyl viologen + 2 H+ | - |
? | |
| 1.2.7.11 | phenylpyruvate + CoA + 2 methyl viologen | enzyme Ape1473/1472, 36% of the activity compared to glyoxylate | Aeropyrum pernix | phenylacetyl-CoA + CO2 + 2 reduced methyl viologen + 2 H+ | - |
? | |
| 1.2.7.11 | pyruvate + CoA + 2 oxidized methyl viologen | enzyme Ape1473/1472, 95% of the activity compared to glyoxylate | Aeropyrum pernix | acetyl-CoA + CO2 + 2 reduced methyl viologen + 2 H+ | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 1.2.7.1 | heterodimer | 1 * 71639 + 1 * 34936, calculated from sequence, enzyme Ape2126/2128 | Aeropyrum pernix |
| 1.2.7.1 | heterodimer | 1 * 72000 + 1 * 35000, SDS-PAGE, enzyme Ape2126/2128 | Aeropyrum pernix |
| 1.2.7.11 | heterodimer | 1 * 67000 + 1 * 34000, SDS-PAGE, enzyme Ape1473/1472 | Aeropyrum pernix |
| 1.2.7.11 | heterodimer | 1 * 67405 + 1 * 34410, calculated from sequence, enzyme Ape1473/1472 | Aeropyrum pernix |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 1.2.7.1 | Ape2126/2128 | ordered locus name | Aeropyrum pernix |
| 1.2.7.1 | OFOR | - |
Aeropyrum pernix |
| 1.2.7.11 | Ape1473/1472 | ordered locus name | Aeropyrum pernix |
| 1.2.7.11 | OFOR | - |
Aeropyrum pernix |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.2.7.1 | 80 | - |
assay at | Aeropyrum pernix |
| 1.2.7.1 | 105 | - |
enzyme Ape2126/2128 | Aeropyrum pernix |
| 1.2.7.11 | 80 | - |
assay at | Aeropyrum pernix |
| 1.2.7.11 | 110 | - |
enzyme Ape1473/1472 | Aeropyrum pernix |
Temperature Range [°C]
| EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.2.7.1 | 80 | 110 | 80°C: 45% of maximal activity, 110°C: 95% of maximal activity, enzyme Ape2126/2128 | Aeropyrum pernix |
| 1.2.7.11 | 90 | 110 | 70°C: 17% of maximal activity, 90°C: about 40% of maximal activity, 110°C: maximal activity, enzyme Ape1473/1472 | Aeropyrum pernix |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.2.7.1 | 80 | - |
20 min, stable, enzyme Ape2126/2128 | Aeropyrum pernix |
| 1.2.7.11 | 80 | - |
20 min, stable, enzyme Ape1473/1472 | Aeropyrum pernix |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.2.7.1 | 8 | - |
enzyme Ape2126/2128 | Aeropyrum pernix |
| 1.2.7.11 | 8.5 | - |
enzyme Ape1473/1472 | Aeropyrum pernix |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.2.7.1 | 6 | 10 | pH 6.0: about 45% of maximal activity, pH 10.0: about 60% of maximal activity, enzyme Ape2126/2128 | Aeropyrum pernix |
| 1.2.7.11 | 7.5 | 10 | pH 7.5: about 70% of maximal activity, pH 10.0: about 70% of maximal activity, enzyme Ape1473/1472 | Aeropyrum pernix |
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