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Literature summary extracted from
- Molecular basis for specificities of reactivating factors for adenosylcobalamin-dependent diol and glycerol dehydratases (2007), FEBS J., 274, 5556-5566.
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 4.2.1.28 | ATP | needed for reactivation | Klebsiella pneumoniae |  |
| 4.2.1.28 | ATP | needed for reactivation | Klebsiella oxytoca | |
| 4.2.1.28 | diol dehydratase-reactivating factor | activates inactive enzymeCN-Cbl complexes, dimeric (alphabbeta) structurea large subunit of 64 kDa and a small subunit of 14 kDa | Klebsiella oxytoca | |
| 4.2.1.28 | diol dehydratase-reactivating factor | activates inactive enzymeCN-Cbl complexes, dimeric (alphabeta) structurea large subunit of 64000 Da and a small subunit of 14000 Da | Klebsiella pneumoniae | |
| 4.2.1.30 | ATP | - |
Klebsiella pneumoniae | |
| 4.2.1.30 | ATP | - |
Klebsiella oxytoca | |
| 4.2.1.30 | glycerol dehydratase-reactivating factor | - |
Klebsiella pneumoniae | |
| 4.2.1.30 | glycerol dehydratase-reactivating factor | - |
Klebsiella oxytoca |
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 4.2.1.28 | expression in Escherichia coli | Klebsiella pneumoniae |
| 4.2.1.28 | expression in Escherichia coli | Klebsiella oxytoca |
| 4.2.1.30 | overexpressed in Escherichia coli | Klebsiella pneumoniae |
| 4.2.1.30 | overexpressed in Escherichia coli | Klebsiella oxytoca |
Crystallization (Commentary)
| EC Number | Crystallization (Comment) | Organism |
|---|---|---|
| 4.2.1.30 | - |
Klebsiella pneumoniae |
| 4.2.1.30 | - |
Klebsiella oxytoca |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 4.2.1.30 | glycerol | suicide inactivation | Klebsiella oxytoca | |
| 4.2.1.30 | glycerol | suicide inactivation | Klebsiella pneumoniae | |
| 4.2.1.30 | O2 | in absence of substrate | Klebsiella oxytoca | |
| 4.2.1.30 | O2 | in absence of substrate | Klebsiella pneumoniae | |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 4.2.1.28 | Mg2+ | needed for reactivation | Klebsiella pneumoniae | |
| 4.2.1.28 | Mg2+ | needed for reactivation | Klebsiella oxytoca | |
| 4.2.1.30 | Mg2+ | essential for glycerol dehydratase-reactivating factor | Klebsiella pneumoniae | |
| 4.2.1.30 | Mg2+ | essential for glycerol dehydratase-reactivating factor | Klebsiella oxytoca | |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 4.2.1.30 | glycerol | Klebsiella pneumoniae | - |
3-hydroxypropanal + H2O | - |
? | |
| 4.2.1.30 | glycerol | Klebsiella oxytoca | - |
3-hydroxypropanal + H2O | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 4.2.1.28 | Klebsiella oxytoca | - |
- |
- |
| 4.2.1.28 | Klebsiella pneumoniae | - |
- |
- |
| 4.2.1.30 | Klebsiella oxytoca | - |
- |
- |
| 4.2.1.30 | Klebsiella pneumoniae | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 4.2.1.28 | - |
Klebsiella pneumoniae |
| 4.2.1.28 | - |
Klebsiella oxytoca |
| 4.2.1.30 | - |
Klebsiella pneumoniae |
| 4.2.1.30 | - |
Klebsiella oxytoca |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 4.2.1.28 | 1,2-ethanediol | - |
Klebsiella pneumoniae | acetaldehyde + H2O | - |
? | |
| 4.2.1.28 | 1,2-ethanediol | - |
Klebsiella oxytoca | acetaldehyde + H2O | - |
? | |
| 4.2.1.28 | 1,2-propanediol | - |
Klebsiella pneumoniae | propanal + H2O | - |
? | |
| 4.2.1.28 | 1,2-propanediol | - |
Klebsiella oxytoca | propanal + H2O | - |
? | |
| 4.2.1.28 | glycerol | - |
Klebsiella pneumoniae | beta-hydroxypropionaldehyde + H2O | - |
? | |
| 4.2.1.28 | glycerol | - |
Klebsiella oxytoca | beta-hydroxypropionaldehyde + H2O | - |
? | |
| 4.2.1.30 | glycerol | - |
Klebsiella pneumoniae | 3-hydroxypropanal + H2O | - |
? | |
| 4.2.1.30 | glycerol | - |
Klebsiella oxytoca | 3-hydroxypropanal + H2O | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 4.2.1.30 | dimer | a large subunit (alpha) of 64000 Da and a small subunit (beta) of 14000 Da | Klebsiella pneumoniae |
| 4.2.1.30 | dimer | a large subunit (alpha) of 64000 Da and a small subunit (beta) of 14000 Da | Klebsiella oxytoca |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 4.2.1.28 | 1,2-propanediol hydro-lyase | - |
Klebsiella pneumoniae |
| 4.2.1.28 | 1,2-propanediol hydro-lyase | - |
Klebsiella oxytoca |
| 4.2.1.28 | diol dehydratase | - |
Klebsiella pneumoniae |
| 4.2.1.28 | diol dehydratase | - |
Klebsiella oxytoca |
| 4.2.1.30 | glycerol dehdydrogenase | - |
Klebsiella pneumoniae |
| 4.2.1.30 | glycerol dehdydrogenase | - |
Klebsiella oxytoca |
| 4.2.1.30 | glycerol hydro-lyase | - |
Klebsiella pneumoniae |
| 4.2.1.30 | glycerol hydro-lyase | - |
Klebsiella oxytoca |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 4.2.1.28 | adenosylcobalamin | - |
Klebsiella pneumoniae | |
| 4.2.1.28 | adenosylcobalamin | - |
Klebsiella oxytoca | |
| 4.2.1.30 | adenosylcobalamin | - |
Klebsiella pneumoniae | |
| 4.2.1.30 | adenosylcobalamin | - |
Klebsiella oxytoca | |
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Release 2023.1 (January 2023)
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