Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Banks, E.C.; Doughty, S.W.; Toms, S.M.; Wheelhouse, R.T.; Nicolaou, A.
    Inhibition of cobalamin-dependent methionine synthase by substituted benzo-fused heterocycles (2007), FEBS J., 274, 287-299.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.1.1.13 1-methyl-5-amino-benzimidazole IC50: more than 0.15 mM Rattus norvegicus
2.1.1.13 1-methyl-5-nitro-7-methoxybenzimidazole IC50: 0.15 mM Rattus norvegicus
2.1.1.13 1-methyl-5-nitro-benzimidazole IC50: more than 0.15 mM Rattus norvegicus
2.1.1.13 1-methylbenzimidazole IC50: more than 0.15 mM Rattus norvegicus
2.1.1.13 2,1,3-benzothiadiazole IC50: more than 0.15 mM Rattus norvegicus
2.1.1.13 2-nitro-1H-benzimidazole IC50: 0.12 mM Rattus norvegicus
2.1.1.13 4-amino-2,1,3-benzothiadiazole IC50: more than 0.15 mM Rattus norvegicus
2.1.1.13 4-nitro-2,1,3-benzothiadiazole IC50: 0.08 mM Rattus norvegicus
2.1.1.13 5-amino-7-methoxy-N1-methylbenzimidazole IC50: 0.095 mM Rattus norvegicus
2.1.1.13 5-amino-7-methoxybenzimidazole IC50: more than 0.15 mM Rattus norvegicus
2.1.1.13 5-aminobenzimidazole IC50: more than 0.15 mM Rattus norvegicus
2.1.1.13 5-methoxybenzimidazole IC50: more than 0.15 mM Rattus norvegicus
2.1.1.13 5-nitro-7-methoxybenzimidazole IC50: 0.1 mM Rattus norvegicus
2.1.1.13 benzimidazole IC50: more than 0.15 mM Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.1.1.13 0.006
-
 L-homocysteine in 50 mM phosphate buffer, pH 7.4, at 37°C Rattus norvegicus
2.1.1.13 0.025
-
 N5-methyltetrahydropteroylmonoglutamate in 50 mM phosphate buffer, pH 7.4, at 37°C Rattus norvegicus

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.13 Rattus norvegicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.1.13 DEAE cellulose batch chromatography and Q-sepharose column chromatography Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.1.1.13 liver
-
 Rattus norvegicus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.13 N5-methyltetrahydropteroylmonoglutamate + L-homocysteine
-
 Rattus norvegicus tetrahydropteroylmonoglutamate + L-methionine
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.1.1.13 5-methyltetrahydrofolate-homocysteine transmethylase
-
 Rattus norvegicus
2.1.1.13 MetS
-
 Rattus norvegicus

Cofactor

EC Number Cofactor Comment Organism Structure
2.1.1.13 vitamin B12 dependent Rattus norvegicus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.1.1.13 0.017
-
 4-nitro-2,1,3-benzothiadiazole in 50 mM phosphate buffer, pH 7.4, at 37°C Rattus norvegicus
2.1.1.13 0.026
-
 2-nitro-1H-benzimidazole in 50 mM phosphate buffer, pH 7.4, at 37°C Rattus norvegicus

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
2.1.1.13 0.08
-
 IC50: 0.08 mM Rattus norvegicus 4-nitro-2,1,3-benzothiadiazole
2.1.1.13 0.095
-
 IC50: 0.095 mM Rattus norvegicus 5-amino-7-methoxy-N1-methylbenzimidazole
2.1.1.13 0.1
-
 IC50: 0.1 mM Rattus norvegicus 5-nitro-7-methoxybenzimidazole
2.1.1.13 0.12
-
 IC50: 0.12 mM Rattus norvegicus 2-nitro-1H-benzimidazole
2.1.1.13 0.15
-
 IC50: 0.15 mM Rattus norvegicus 1-methyl-5-nitro-7-methoxybenzimidazole
2.1.1.13 0.15
-
 IC50: more than 0.15 mM Rattus norvegicus benzimidazole
2.1.1.13 0.15
-
 IC50: more than 0.15 mM Rattus norvegicus 1-methylbenzimidazole
2.1.1.13 0.15
-
 IC50: more than 0.15 mM Rattus norvegicus 5-aminobenzimidazole
2.1.1.13 0.15
-
 IC50: more than 0.15 mM Rattus norvegicus 5-methoxybenzimidazole
2.1.1.13 0.15
-
 IC50: more than 0.15 mM Rattus norvegicus 1-methyl-5-nitro-benzimidazole
2.1.1.13 0.15
-
 IC50: more than 0.15 mM Rattus norvegicus 1-methyl-5-amino-benzimidazole
2.1.1.13 0.15
-
 IC50: more than 0.15 mM Rattus norvegicus 5-amino-7-methoxybenzimidazole
2.1.1.13 0.15
-
 IC50: more than 0.15 mM Rattus norvegicus 2,1,3-benzothiadiazole
2.1.1.13 0.15
-
 IC50: more than 0.15 mM Rattus norvegicus 4-amino-2,1,3-benzothiadiazole