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Literature summary extracted from

  • Yamanishi, Y.; Mihara, H.; Osaki, M.; Muramatsu, H.; Esaki, N.; Sato, T.; Hizukuri, Y.; Goto, S.; Kanehisa, M.
    Prediction of missing enzyme genes in a bacterial metabolic network. Reconstruction of the lysine-degradation pathway of Pseudomonas aeruginosa (2007), FEBS J., 274, 2262-2273.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.2.1.20 into pET21a(+) vector, proteins with a C-terminal His6-tag expressed in Escherichia coli BL21(DE3) Pseudomonas aeruginosa PAO1
2.6.1.48 cloned by PCR and expressed in Escherichia coli as a C-terminal histidine-tagged fusion protein Pseudomonas aeruginosa
2.6.1.48 expressed in Escherichia coli BL21(DE3) cells Pseudomonas aeruginosa

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.2.1.20 53000
-
 SDS-PAGE Pseudomonas aeruginosa PAO1
2.6.1.48 46000
-
 SDS-PAGE Pseudomonas aeruginosa
2.6.1.48 46000
-
 x * 46000, SDS-PAGE Pseudomonas aeruginosa
2.6.1.48 46290
-
 calculated from amino acid sequence Pseudomonas aeruginosa

Organism

EC Number Organism UniProt Comment Textmining
1.2.1.20 Pseudomonas aeruginosa PAO1
-
-
-
2.6.1.48 Pseudomonas aeruginosa
-
-
-
2.6.1.48 Pseudomonas aeruginosa Q9I6M4
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.2.1.20 to homogeneity as a C-terminal histidine-tagged fusion protein Pseudomonas aeruginosa PAO1
2.6.1.48 His-Bind column chromatography Pseudomonas aeruginosa
2.6.1.48 recombinant Pseudomonas aeruginosa

Storage Stability

EC Number Storage Stability Organism
1.2.1.20 -80°C, 20 mm Tris-HCl buffer, pH 8.0 Pseudomonas aeruginosa PAO1

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.1.20 glutarate semialdehyde + NADP+ + H2O
-
 Pseudomonas aeruginosa PAO1 glutarate + NADPH
-
 ?
2.6.1.48 5-aminopentanoate + 2-oxoglutarate
-
 Pseudomonas aeruginosa 5-oxovalerate + L-glutamate
-
 ?

Subunits

EC Number Subunits Comment Organism
1.2.1.20 monomer 1 * 53000, SDS-PAGE Pseudomonas aeruginosa PAO1
2.6.1.48 ? x * 46000, SDS-PAGE Pseudomonas aeruginosa

Synonyms

EC Number Synonyms Comment Organism
1.2.1.20 glutarate semialdehyde dehydrogenase
-
 Pseudomonas aeruginosa PAO1
2.6.1.48 5-aminovalerate aminotransferase
-
 Pseudomonas aeruginosa
2.6.1.48 PA0266
-
 Pseudomonas aeruginosa
2.6.1.48 putative 5-aminovalerate aminotransferase
-
 Pseudomonas aeruginosa

Cofactor

EC Number Cofactor Comment Organism Structure
1.2.1.20 NADP+
-
 Pseudomonas aeruginosa PAO1