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Literature summary extracted from

  • Zaffagnini, M.; Michelet, L.; Marchand, C.; Sparla, F.; Decottignies, P.; Le Marechal, P.; Miginiac-Maslow, M.; Noctor, G.; Trost, P.; Lemaire, S.D.
    The thioredoxin-independent isoform of chloroplastic glyceraldehyde-3-phosphate dehydrogenase is selectively regulated by glutathionylation (2007), FEBS J., 274, 212-226.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.2.1.12 for expression in Escherichia coli BL21DE Arabidopsis thaliana

Protein Variants

EC Number Protein Variants Comment Organism
1.2.1.12 C153S mutant, having only one cysteine in the catalytic site, is oxidized and glutathionylated similarly to the wild-type enzyme Arabidopsis thaliana

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.2.1.12 CuCl2 1 mM Arabidopsis thaliana
1.2.1.12 Diamide 1 mM Arabidopsis thaliana
1.2.1.12 glutathione 5 mM oxidized glutathione, GSSG, formation of mixed disulfide between glutathione and A4-GAPDH results in the inhibition of enzyme activity Arabidopsis thaliana
1.2.1.12 glutathione formation of mixed disulfide between glutathione and GAPDH results in the inhibition of enzyme activity Spinacia oleracea
1.2.1.12 H2O2 1 mM Arabidopsis thaliana
1.2.1.12 H2O2
-
 Spinacia oleracea

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.2.1.12 chloroplast
-
 Spinacia oleracea 9507
-
1.2.1.12 chloroplast
-
 Arabidopsis thaliana 9507
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.2.1.12 36140
-
 theoretical mass of the A subunit Spinacia oleracea
1.2.1.12 39360
-
 theoretical mass of the B subunit Spinacia oleracea

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.2.1.12 3-phospho-D-glyceroyl phosphate + NADH + H+ Spinacia oleracea
-
 D-glyceraldehyde 3-phosphate + phosphate + NAD+
-
 ?
1.2.1.12 3-phospho-D-glyceroyl phosphate + NADH + H+ Arabidopsis thaliana
-
 D-glyceraldehyde 3-phosphate + phosphate + NAD+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.2.1.12 Arabidopsis thaliana
-
-
-
1.2.1.12 Spinacia oleracea
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
1.2.1.12 glutathionylation
-
 Arabidopsis thaliana

Purification (Commentary)

EC Number Purification (Comment) Organism
1.2.1.12
-
 Arabidopsis thaliana
1.2.1.12 native GAPDH is purified from spinach chloroplasts Spinacia oleracea

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.1.12 3-phospho-D-glyceroyl phosphate + NADH + H+
-
 Spinacia oleracea D-glyceraldehyde 3-phosphate + phosphate + NAD+
-
 ?
1.2.1.12 3-phospho-D-glyceroyl phosphate + NADH + H+
-
 Arabidopsis thaliana D-glyceraldehyde 3-phosphate + phosphate + NAD+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.2.1.12 heterotetramer A2B2-GAPDH, light conformation, B subunits are almost identical to A subunits, expect for the presence of a C-terminal extension containing a pair of cysteines, which is the target of TRX regulation Spinacia oleracea
1.2.1.12 homotetramer A4-GAPDH Arabidopsis thaliana
1.2.1.12 multimer A8B8-GAPDH, dark conformation, B subunits are almost identical to A subunits, expect for the presence of a C-terminal extension containing a pair of cysteines, which is the target of TRX regulation Spinacia oleracea

Synonyms

EC Number Synonyms Comment Organism
1.2.1.12 A4-GAPDH
-
 Arabidopsis thaliana
1.2.1.12 A4-glyceraldehyde-3-phosphate dehydrogenase
-
 Arabidopsis thaliana
1.2.1.12 AnBn-GAPDH
-
 Spinacia oleracea
1.2.1.12 GAPDH
-
 Spinacia oleracea
1.2.1.12 GAPDH
-
 Arabidopsis thaliana
1.2.1.12 glyceraldehyde-3-phosphate dehydrogenase
-
 Spinacia oleracea
1.2.1.12 glyceraldehyde-3-phosphate dehydrogenase
-
 Arabidopsis thaliana

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.2.1.12 25
-
 activity assay Arabidopsis thaliana

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.2.1.12 7.9
-
 activity assay Arabidopsis thaliana

Cofactor

EC Number Cofactor Comment Organism Structure
1.2.1.12 NADH
-
 Spinacia oleracea
1.2.1.12 NADH
-
 Arabidopsis thaliana
1.2.1.12 NADPH
-
 Spinacia oleracea