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Literature summary extracted from

  • Andersen, G.; Andersen, B.; Dobritzsch, D.; Schnackerz, K.D.; Piskur, J.
    A gene duplication led to specialized gamma-aminobutyrate and beta-alanine aminotransferase in yeast (2007), FEBS J., 274, 1804-1817.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.6.1.2 expression as His-tagged protein Lachancea kluyveri
2.6.1.19 expressed in Escherichia coli BL-21(DE3) cells Lachancea kluyveri
2.6.1.19 expression as His-tagged protein Saccharomyces cerevisiae
2.6.1.19 expression as His-tagged protein Schizosaccharomyces pombe
2.6.1.19 expression as His-tagged protein Lachancea kluyveri

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.6.1.2 2.1
-
 2-oxoglutarate pH 8.0, 30°C Lachancea kluyveri
2.6.1.2 7.6
-
 beta-Alanine
-
 Lachancea kluyveri
2.6.1.2 7.6
-
 beta-Alanine pH 8.0, 30°C Lachancea kluyveri
2.6.1.19 0.22
-
 2-oxoglutarate pH 8.0, 30°C Lachancea kluyveri
2.6.1.19 0.22
-
 2-oxoglutarate in 100 mM potassium phosphate (pH 8.0), 0.1 mM pyridoxal 5'-phosphate Lachancea kluyveri
2.6.1.19 2.1
-
 2-oxoglutarate pH 8.0, 30°C Lachancea kluyveri
2.6.1.19 3.2
-
 gamma-aminobutanoate pH 8.0, 30°C Lachancea kluyveri
2.6.1.19 3.2
-
 4-aminobutanoate in 100 mM potassium phosphate (pH 8.0), 0.1 mM pyridoxal 5'-phosphate Lachancea kluyveri
2.6.1.19 7.6
-
 beta-Alanine pH 8.0, 30°C Lachancea kluyveri

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.6.1.2 55500
-
 2 * 55500, gel filtration Lachancea kluyveri
2.6.1.2 56000
-
 2 * 56000, SDS-PAGE Lachancea kluyveri
2.6.1.2 111000
-
 gel filtration Lachancea kluyveri
2.6.1.2 111000
-
 PAGE Lachancea kluyveri
2.6.1.19 56000
-
 2 * 56000, SDS-PAGE Lachancea kluyveri
2.6.1.19 98000
-
 PAGE Lachancea kluyveri
2.6.1.19 111000
-
 PAGE Lachancea kluyveri

Organism

EC Number Organism UniProt Comment Textmining
2.6.1.2 Lachancea kluyveri
-
-
-
2.6.1.2 Lachancea kluyveri A5H0J5 isoform Pyd4, involved in both beta-alanine and gamma-aminobutanoic acid transamination
-
2.6.1.19 Lachancea kluyveri
-
-
-
2.6.1.19 Lachancea kluyveri A5H0J5 isoform Pyd4, involved in both beta-alanine and gamma-aminobutanoic acid transamination
-
2.6.1.19 Lachancea kluyveri A5H0J6 isoform Uga1
-
2.6.1.19 Saccharomyces cerevisiae P17649 isoform Uga1
-
2.6.1.19 Schizosaccharomyces pombe O13837 isoform Uga1
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.6.1.2 Sepharose S12 gel filtration and nickel-chelating Sepharose column chromatography Lachancea kluyveri
2.6.1.19 nickel-chelating Sepharose chromatography and Sepharose S12 gel filtration Lachancea kluyveri

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.6.1.2 0.75
-
 substrate gamma-aminobutanoate, continuous assay with succinate dehydrogenase, pH 8.0, 30°C Lachancea kluyveri
2.6.1.2 1.6
-
 substrate gamma-aminobutanoate, continuous assay using malonic semialdehyde decarboxylase and alcohol dehydrogenase, pH 8.0, 30°C Lachancea kluyveri
2.6.1.2 3.52
-
 substrate beta-alanine, discontinuous determination of glutamate using glutamate dehydrogenase in the presence of 10 M hydrazine, pH 8.0, 30°C Lachancea kluyveri
2.6.1.2 4.5
-
 substrate beta-alanine, continuous assay using malonic semialdehyde decarboxylase and alcohol dehydrogenase, pH 8.0, 30°C Lachancea kluyveri
2.6.1.19 0.1
-
 substrate beta-alanine, discontinuous determination of glutamate using glutamate dehydrogenase in the presence of 10 M hydrazine, pH 8.0, 30°C Lachancea kluyveri
2.6.1.19 0.13
-
 substrate beta-alanine, continuous assay using malonic semialdehyde decarboxylase and alcohol dehydrogenase, pH 8.0, 30°C Lachancea kluyveri
2.6.1.19 0.14
-
 substrate beta-alanine, discontinuous determination of glutamate using glutamate dehydrogenase in the presence of 10 M hydrazine, pH 8.0, 30°C Schizosaccharomyces pombe
2.6.1.19 0.15
-
 substrate beta-alanine, discontinuous determination of glutamate using glutamate dehydrogenase in the presence of 10 M hydrazine, pH 8.0, 30°C Saccharomyces cerevisiae
2.6.1.19 0.75
-
 substrate gamma-aminobutanoate, continuous assay with succinate dehydrogenase, pH 8.0, 30°C Lachancea kluyveri
2.6.1.19 1.6
-
 substrate gamma-aminobutanoate, continuous assay using malonic semialdehyde decarboxylase and alcohol dehydrogenase, pH 8.0, 30°C Lachancea kluyveri
2.6.1.19 3.52
-
 substrate beta-alanine, discontinuous determination of glutamate using glutamate dehydrogenase in the presence of 10 M hydrazine, pH 8.0, 30°C Lachancea kluyveri
2.6.1.19 3.6
-
 substrate gamma-aminobutanoate, continuous assay using malonic semialdehyde decarboxylase and alcohol dehydrogenase, pH 8.0, 30°C Saccharomyces cerevisiae
2.6.1.19 3.67
-
 continuous assay using malonic semialdehyde decarboxylase and alcohol dehydrogenase, pH 8.0, 30°C Schizosaccharomyces pombe
2.6.1.19 4.5
-
 substrate beta-alanine, continuous assay using malonic semialdehyde decarboxylase and alcohol dehydrogenase, pH 8.0, 30°C Lachancea kluyveri
2.6.1.19 12.8
-
 using 4-aminobutanoate as substrate Lachancea kluyveri
2.6.1.19 12.8
-
 substrate gamma-aminobutanoate, continuous assay with succinate dehydrogenase, pH 8.0, 30°C Lachancea kluyveri

Storage Stability

EC Number Storage Stability Organism
2.6.1.2 4°C, 12 days, 57% residual activity Lachancea kluyveri
2.6.1.19 4°C, 12 days, 57% residual activity Saccharomyces cerevisiae
2.6.1.19 4°C, 12 days, 57% residual activity Schizosaccharomyces pombe
2.6.1.19 4°C, 12 days, 57% residual activity Lachancea kluyveri

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.6.1.2 4-aminobutanoate + 2-oxoglutarate
-
 Lachancea kluyveri 4-oxobutanoate + L-glutamate
-
 ?
2.6.1.2 beta-alanine + 2-oxoglutarate
-
 Lachancea kluyveri malonic semialdehyde + L-glutamate
-
 ?
2.6.1.19 4-aminobutanoate + 2-oxoglutarate
-
 Lachancea kluyveri 4-oxobutanoate + L-glutamate
-
 r
2.6.1.19 4-aminobutanoate + 2-oxoglutarate
-
 Saccharomyces cerevisiae 4-oxobutanoate + L-glutamate
-
 ?
2.6.1.19 4-aminobutanoate + 2-oxoglutarate
-
 Schizosaccharomyces pombe 4-oxobutanoate + L-glutamate
-
 ?
2.6.1.19 4-aminobutanoate + 2-oxoglutarate
-
 Lachancea kluyveri 4-oxobutanoate + L-glutamate
-
 ?
2.6.1.19 beta-alanine + 2-oxoglutarate
-
 Saccharomyces cerevisiae malonic semialdehyde + L-glutamate
-
 ?
2.6.1.19 beta-alanine + 2-oxoglutarate
-
 Schizosaccharomyces pombe malonic semialdehyde + L-glutamate
-
 ?
2.6.1.19 beta-alanine + 2-oxoglutarate
-
 Lachancea kluyveri malonic semialdehyde + L-glutamate
-
 ?
2.6.1.19 additional information does not use pyruvate as amino acceptor Lachancea kluyveri ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.6.1.2 dimer 2 * 56000, SDS-PAGE Lachancea kluyveri
2.6.1.2 dimer 2 * 55500, gel filtration Lachancea kluyveri
2.6.1.19 dimer 2 * 56000, SDS-PAGE Lachancea kluyveri

Synonyms

EC Number Synonyms Comment Organism
2.6.1.2 ALT
-
 Lachancea kluyveri
2.6.1.2 beta-alanine aminotransferase
-
 Lachancea kluyveri
2.6.1.2 SkPyd4p
-
 Lachancea kluyveri
2.6.1.19 gamma-aminobutyrate aminotransferase
-
 Lachancea kluyveri
2.6.1.19 SkUga1p
-
 Lachancea kluyveri

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.6.1.2 8
-
-
 Lachancea kluyveri
2.6.1.19 8
-
-
 Lachancea kluyveri

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.6.1.2 7 9 more than 70% of maximum activity Lachancea kluyveri
2.6.1.19 7 9 more than 70% of maximum activity Lachancea kluyveri

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
2.6.1.2 7 9 70% of activity remaining at pH values of 7 and 9 Lachancea kluyveri

Cofactor

EC Number Cofactor Comment Organism Structure
2.6.1.2 pyridoxal 5'-phosphate required Lachancea kluyveri
2.6.1.19 pyridoxal 5'-phosphate
-
 Lachancea kluyveri