EC Number | Cloned (Comment) | Organism |
---|---|---|
2.6.1.2 | expression as His-tagged protein | Lachancea kluyveri |
2.6.1.19 | expressed in Escherichia coli BL-21(DE3) cells | Lachancea kluyveri |
2.6.1.19 | expression as His-tagged protein | Saccharomyces cerevisiae |
2.6.1.19 | expression as His-tagged protein | Schizosaccharomyces pombe |
2.6.1.19 | expression as His-tagged protein | Lachancea kluyveri |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.6.1.2 | 2.1 | - |
2-oxoglutarate | pH 8.0, 30°C | Lachancea kluyveri | |
2.6.1.2 | 7.6 | - |
beta-Alanine | - |
Lachancea kluyveri | |
2.6.1.2 | 7.6 | - |
beta-Alanine | pH 8.0, 30°C | Lachancea kluyveri | |
2.6.1.19 | 0.22 | - |
2-oxoglutarate | pH 8.0, 30°C | Lachancea kluyveri | |
2.6.1.19 | 0.22 | - |
2-oxoglutarate | in 100 mM potassium phosphate (pH 8.0), 0.1 mM pyridoxal 5'-phosphate | Lachancea kluyveri | |
2.6.1.19 | 2.1 | - |
2-oxoglutarate | pH 8.0, 30°C | Lachancea kluyveri | |
2.6.1.19 | 3.2 | - |
gamma-aminobutanoate | pH 8.0, 30°C | Lachancea kluyveri | |
2.6.1.19 | 3.2 | - |
4-aminobutanoate | in 100 mM potassium phosphate (pH 8.0), 0.1 mM pyridoxal 5'-phosphate | Lachancea kluyveri | |
2.6.1.19 | 7.6 | - |
beta-Alanine | pH 8.0, 30°C | Lachancea kluyveri |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
2.6.1.2 | 55500 | - |
2 * 55500, gel filtration | Lachancea kluyveri |
2.6.1.2 | 56000 | - |
2 * 56000, SDS-PAGE | Lachancea kluyveri |
2.6.1.2 | 111000 | - |
gel filtration | Lachancea kluyveri |
2.6.1.2 | 111000 | - |
PAGE | Lachancea kluyveri |
2.6.1.19 | 56000 | - |
2 * 56000, SDS-PAGE | Lachancea kluyveri |
2.6.1.19 | 98000 | - |
PAGE | Lachancea kluyveri |
2.6.1.19 | 111000 | - |
PAGE | Lachancea kluyveri |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.6.1.2 | Lachancea kluyveri | - |
- |
- |
2.6.1.2 | Lachancea kluyveri | A5H0J5 | isoform Pyd4, involved in both beta-alanine and gamma-aminobutanoic acid transamination | - |
2.6.1.19 | Lachancea kluyveri | - |
- |
- |
2.6.1.19 | Lachancea kluyveri | A5H0J5 | isoform Pyd4, involved in both beta-alanine and gamma-aminobutanoic acid transamination | - |
2.6.1.19 | Lachancea kluyveri | A5H0J6 | isoform Uga1 | - |
2.6.1.19 | Saccharomyces cerevisiae | P17649 | isoform Uga1 | - |
2.6.1.19 | Schizosaccharomyces pombe | O13837 | isoform Uga1 | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.6.1.2 | Sepharose S12 gel filtration and nickel-chelating Sepharose column chromatography | Lachancea kluyveri |
2.6.1.19 | nickel-chelating Sepharose chromatography and Sepharose S12 gel filtration | Lachancea kluyveri |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
2.6.1.2 | 0.75 | - |
substrate gamma-aminobutanoate, continuous assay with succinate dehydrogenase, pH 8.0, 30°C | Lachancea kluyveri |
2.6.1.2 | 1.6 | - |
substrate gamma-aminobutanoate, continuous assay using malonic semialdehyde decarboxylase and alcohol dehydrogenase, pH 8.0, 30°C | Lachancea kluyveri |
2.6.1.2 | 3.52 | - |
substrate beta-alanine, discontinuous determination of glutamate using glutamate dehydrogenase in the presence of 10 M hydrazine, pH 8.0, 30°C | Lachancea kluyveri |
2.6.1.2 | 4.5 | - |
substrate beta-alanine, continuous assay using malonic semialdehyde decarboxylase and alcohol dehydrogenase, pH 8.0, 30°C | Lachancea kluyveri |
2.6.1.19 | 0.1 | - |
substrate beta-alanine, discontinuous determination of glutamate using glutamate dehydrogenase in the presence of 10 M hydrazine, pH 8.0, 30°C | Lachancea kluyveri |
2.6.1.19 | 0.13 | - |
substrate beta-alanine, continuous assay using malonic semialdehyde decarboxylase and alcohol dehydrogenase, pH 8.0, 30°C | Lachancea kluyveri |
2.6.1.19 | 0.14 | - |
substrate beta-alanine, discontinuous determination of glutamate using glutamate dehydrogenase in the presence of 10 M hydrazine, pH 8.0, 30°C | Schizosaccharomyces pombe |
2.6.1.19 | 0.15 | - |
substrate beta-alanine, discontinuous determination of glutamate using glutamate dehydrogenase in the presence of 10 M hydrazine, pH 8.0, 30°C | Saccharomyces cerevisiae |
2.6.1.19 | 0.75 | - |
substrate gamma-aminobutanoate, continuous assay with succinate dehydrogenase, pH 8.0, 30°C | Lachancea kluyveri |
2.6.1.19 | 1.6 | - |
substrate gamma-aminobutanoate, continuous assay using malonic semialdehyde decarboxylase and alcohol dehydrogenase, pH 8.0, 30°C | Lachancea kluyveri |
2.6.1.19 | 3.52 | - |
substrate beta-alanine, discontinuous determination of glutamate using glutamate dehydrogenase in the presence of 10 M hydrazine, pH 8.0, 30°C | Lachancea kluyveri |
2.6.1.19 | 3.6 | - |
substrate gamma-aminobutanoate, continuous assay using malonic semialdehyde decarboxylase and alcohol dehydrogenase, pH 8.0, 30°C | Saccharomyces cerevisiae |
2.6.1.19 | 3.67 | - |
continuous assay using malonic semialdehyde decarboxylase and alcohol dehydrogenase, pH 8.0, 30°C | Schizosaccharomyces pombe |
2.6.1.19 | 4.5 | - |
substrate beta-alanine, continuous assay using malonic semialdehyde decarboxylase and alcohol dehydrogenase, pH 8.0, 30°C | Lachancea kluyveri |
2.6.1.19 | 12.8 | - |
using 4-aminobutanoate as substrate | Lachancea kluyveri |
2.6.1.19 | 12.8 | - |
substrate gamma-aminobutanoate, continuous assay with succinate dehydrogenase, pH 8.0, 30°C | Lachancea kluyveri |
EC Number | Storage Stability | Organism |
---|---|---|
2.6.1.2 | 4°C, 12 days, 57% residual activity | Lachancea kluyveri |
2.6.1.19 | 4°C, 12 days, 57% residual activity | Saccharomyces cerevisiae |
2.6.1.19 | 4°C, 12 days, 57% residual activity | Schizosaccharomyces pombe |
2.6.1.19 | 4°C, 12 days, 57% residual activity | Lachancea kluyveri |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.6.1.2 | 4-aminobutanoate + 2-oxoglutarate | - |
Lachancea kluyveri | 4-oxobutanoate + L-glutamate | - |
? | |
2.6.1.2 | beta-alanine + 2-oxoglutarate | - |
Lachancea kluyveri | malonic semialdehyde + L-glutamate | - |
? | |
2.6.1.19 | 4-aminobutanoate + 2-oxoglutarate | - |
Lachancea kluyveri | 4-oxobutanoate + L-glutamate | - |
r | |
2.6.1.19 | 4-aminobutanoate + 2-oxoglutarate | - |
Saccharomyces cerevisiae | 4-oxobutanoate + L-glutamate | - |
? | |
2.6.1.19 | 4-aminobutanoate + 2-oxoglutarate | - |
Schizosaccharomyces pombe | 4-oxobutanoate + L-glutamate | - |
? | |
2.6.1.19 | 4-aminobutanoate + 2-oxoglutarate | - |
Lachancea kluyveri | 4-oxobutanoate + L-glutamate | - |
? | |
2.6.1.19 | beta-alanine + 2-oxoglutarate | - |
Saccharomyces cerevisiae | malonic semialdehyde + L-glutamate | - |
? | |
2.6.1.19 | beta-alanine + 2-oxoglutarate | - |
Schizosaccharomyces pombe | malonic semialdehyde + L-glutamate | - |
? | |
2.6.1.19 | beta-alanine + 2-oxoglutarate | - |
Lachancea kluyveri | malonic semialdehyde + L-glutamate | - |
? | |
2.6.1.19 | additional information | does not use pyruvate as amino acceptor | Lachancea kluyveri | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
2.6.1.2 | dimer | 2 * 56000, SDS-PAGE | Lachancea kluyveri |
2.6.1.2 | dimer | 2 * 55500, gel filtration | Lachancea kluyveri |
2.6.1.19 | dimer | 2 * 56000, SDS-PAGE | Lachancea kluyveri |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.6.1.2 | ALT | - |
Lachancea kluyveri |
2.6.1.2 | beta-alanine aminotransferase | - |
Lachancea kluyveri |
2.6.1.2 | SkPyd4p | - |
Lachancea kluyveri |
2.6.1.19 | gamma-aminobutyrate aminotransferase | - |
Lachancea kluyveri |
2.6.1.19 | SkUga1p | - |
Lachancea kluyveri |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.6.1.2 | 8 | - |
- |
Lachancea kluyveri |
2.6.1.19 | 8 | - |
- |
Lachancea kluyveri |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
2.6.1.2 | 7 | 9 | more than 70% of maximum activity | Lachancea kluyveri |
2.6.1.19 | 7 | 9 | more than 70% of maximum activity | Lachancea kluyveri |
EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|---|
2.6.1.2 | 7 | 9 | 70% of activity remaining at pH values of 7 and 9 | Lachancea kluyveri |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
2.6.1.2 | pyridoxal 5'-phosphate | required | Lachancea kluyveri | |
2.6.1.19 | pyridoxal 5'-phosphate | - |
Lachancea kluyveri |