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Literature summary extracted from

  • Kaestner, U.; Hallmen, C.; Wiese, M.; Leistner, E.; Drewke, C.
    The human pyridoxal kinase, a plausible target for ginkgotoxin from Ginkgo biloba (2007), FEBS J., 274, 1036-1045.
    View publication on PubMed

Application

EC Number Application Comment Organism
2.7.1.35 medicine ingestion of 4'-O-methylpyridoxine, i.e. ginkgotoxin, triggers epileptic convulsions and other neuronal symptoms. 4'-O-Methylpyridoxine is an competitive inhibitor of pyridoxal kinase and leads to temporarily reduced pyridoxal phosphate formation in vitro and possibly in vivo Homo sapiens

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.35
-
 Homo sapiens
2.7.1.35 expressed in Escherichia coli strain BL21 (DE3) Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.35 4'-deoxypyridoxine
-
 Homo sapiens
2.7.1.35 4'-O-methylpyridoxine i.e. ginkgotoxin. Treatment leads to temporarily reduced pyridoxal phosphate formation in vitro and possibly in vivo Homo sapiens
2.7.1.35 ginkgotoxin
-
 Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.35 0.0028
-
 pyridoxal in 70 mM potassium phosphate buffer, pH 6.2 Homo sapiens
2.7.1.35 0.00495
-
 4'-O-methylpyridoxine pH 6.2, 37°C Homo sapiens
2.7.1.35 0.00495
-
 ginkgotoxin in 70 mM potassium phosphate buffer, pH 6.2 Homo sapiens
2.7.1.35 0.00987
-
 pyridoxine pH 6.2, 37°C Homo sapiens
2.7.1.35 0.00987
-
 pyridoxine in 70 mM potassium phosphate buffer, pH 6.2 Homo sapiens
2.7.1.35 0.0216
-
 4'-deoxypyridoxine pH 6.2, 37°C Homo sapiens
2.7.1.35 0.0216
-
 4'-deoxypyridoxine in 70 mM potassium phosphate buffer, pH 6.2 Homo sapiens
2.7.1.35 0.0587
-
 pyridoxal pH 6.2, 37°C Homo sapiens
2.7.1.35 0.126
-
 pyridoxamine pH 6.2, 37°C Homo sapiens
2.7.1.35 0.126
-
 pyridoxamine in 70 mM potassium phosphate buffer, pH 6.2 Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.35 Homo sapiens O00764
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.35 pyridoxyl-EAH-Sepharose 4B column chromatography Homo sapiens
2.7.1.35 recombinant enzyme Homo sapiens

Storage Stability

EC Number Storage Stability Organism
2.7.1.35 -20°C, recombinant enzyme, stable for 21 days Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.35 ATP + 4'-deoxypyridoxine
-
 Homo sapiens ADP + 4'-deoxypyridoxine 5'-phosphate
-
 ?
2.7.1.35 ATP + 4'-O-methylpyridoxine
-
 Homo sapiens ADP + 4'-O-methylpyridoxine 5'-phosphate
-
 ?
2.7.1.35 ATP + ginkgotoxin
-
 Homo sapiens ADP + ginkgotoxin 5'-phosphate
-
 ?
2.7.1.35 ATP + pyridoxal
-
 Homo sapiens ADP + pyridoxal 5'-phosphate
-
 ?
2.7.1.35 ATP + pyridoxal no substrate: GTP Homo sapiens ADP + pyridoxal 5'-phosphate
-
 ?
2.7.1.35 ATP + pyridoxamine
-
 Homo sapiens ADP + pyridoxamine 5'-phosphate
-
 ?
2.7.1.35 ATP + pyridoxine
-
 Homo sapiens ADP + pyridoxine 5'-phosphate
-
 ?
2.7.1.35 additional information no activity with GTP Homo sapiens ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.7.1.35 PKH
-
 Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.1.35 45
-
-
 Homo sapiens

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.7.1.35 -20
-
 stable at -20°C for approximately 21 days Homo sapiens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.7.1.35 0.46
-
 ginkgotoxin in 70 mM potassium phosphate buffer, pH 6.2 Homo sapiens
2.7.1.35 0.46
-
 4'-O-methylpyridoxine pH 6.2, 37°C Homo sapiens
2.7.1.35 0.79
-
 pyridoxamine in 70 mM potassium phosphate buffer, pH 6.2 Homo sapiens
2.7.1.35 0.79
-
 pyridoxamine pH 6.2, 37°C Homo sapiens
2.7.1.35 0.995
-
 pyridoxal pH 6.2, 37°C Homo sapiens
2.7.1.35 1.233
-
 pyridoxine pH 6.2, 37°C Homo sapiens
2.7.1.35 1.535
-
 4'-deoxypyridoxine pH 6.2, 37°C Homo sapiens
2.7.1.35 2.94
-
 4'-deoxypyridoxine in 70 mM potassium phosphate buffer, pH 6.2 Homo sapiens
2.7.1.35 6.08
-
 pyridoxal in 70 mM potassium phosphate buffer, pH 6.2 Homo sapiens
2.7.1.35 6.08
-
 pyridoxine in 70 mM potassium phosphate buffer, pH 6.2 Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.35 5.8 6.3
-
 Homo sapiens

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.1.35 ATP
-
 Homo sapiens

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.7.1.35 0.000414
-
 ginkgotoxin
-
 Homo sapiens
2.7.1.35 0.000414
-
 4'-O-methylpyridoxine pH 6.2, 37°C Homo sapiens
2.7.1.35 0.0574
-
 4'-deoxypyridoxine
-
 Homo sapiens
2.7.1.35 0.0574
-
 4'-deoxypyridoxine pH 6.2, 37°C Homo sapiens