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Literature summary extracted from

  • Yasmin, L.; Jansson, A.L.; Panahandeh, T.; Palmer, R.H.; Francis, M.S.; Hallberg, B.
    Delineation of exoenzyme S residues that mediate the interaction with 14-3-3 and its biological activity (2006), FEBS J., 273, 638-646.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.4.2.30 protein 14-3-3 required, interaction analysis, interaction equires residues L426, D427, and L428, overview, binding study of mutant enzymes, overview Pseudomonas aeruginosa

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.2.30 expression of various GST-fusion protein constructs of ExoS mutants in HeLa cells Pseudomonas aeruginosa

Protein Variants

EC Number Protein Variants Comment Organism
2.4.2.30 A425K site-directed mutagenesis, phenotypic analysis Pseudomonas aeruginosa
2.4.2.30 D424A site-directed mutagenesis, phenotypic analysis Pseudomonas aeruginosa
2.4.2.30 D424A/D427A site-directed mutagenesis, reduced interaction with 14-3-3, phenotypic analysis Pseudomonas aeruginosa
2.4.2.30 D424A/L426A/D427A/L428A site-directed mutagenesis, the mutant shows a highly reduced expression level, phenotypic analysis Pseudomonas aeruginosa
2.4.2.30 D427A site-directed mutagenesis, the mutant shows a reduced expression level, phenotypic analysis Pseudomonas aeruginosa
2.4.2.30 G421A site-directed mutagenesis, phenotypic analysis Pseudomonas aeruginosa
2.4.2.30 L422A site-directed mutagenesis, phenotypic analysis Pseudomonas aeruginosa
2.4.2.30 L423A site-directed mutagenesis, phenotypic analysis Pseudomonas aeruginosa
2.4.2.30 L426A site-directed mutagenesis, phenotypic analysis Pseudomonas aeruginosa
2.4.2.30 L426A/D427A site-directed mutagenesis, the mutant shows a highly reduced expression level and slightly reduced cytotoxicity, no interaction with 14-3-3, phenotypic analysis Pseudomonas aeruginosa
2.4.2.30 L426A/D427A/L428A site-directed mutagenesis, the mutant shows a highly reduced expression level and cytotoxicity, no interaction with 14-3-3, phenotypic analysis Pseudomonas aeruginosa
2.4.2.30 L428A site-directed mutagenesis, reduced interaction with 14-3-3, the mutant shows highly reduced cytotoxicity, phenotypic analysis Pseudomonas aeruginosa
2.4.2.30 Q420A site-directed mutagenesis, phenotypic analysis Pseudomonas aeruginosa
2.4.2.30 S419I site-directed mutagenesis, phenotypic analysis Pseudomonas aeruginosa

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.2.30 additional information Pseudomonas aeruginosa the bacterial enzyme shows the ability to mediate cell death in the host and is a toxin, residues 426-428 are required for this activity ?
-
 ?
2.4.2.30 NAD+ + (ADP-D-ribosyl)n-acceptor Pseudomonas aeruginosa
-
 nicotinamide + (ADP-D-ribosyl)n+1-acceptor
-
 ?
2.4.2.30 NAD+ + Ras Pseudomonas aeruginosa interaction requires residue Leu428 nicotinamide + (ADP-D-ribosyl)-Ras
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.2.30 Pseudomonas aeruginosa
-
 gene exoS
-

Reaction

EC Number Reaction Comment Organism Reaction ID
2.4.2.30 NAD+ + (ADP-D-ribosyl)n-acceptor = nicotinamide + (ADP-D-ribosyl)n+1-acceptor + H+ L428 is essential for activity Pseudomonas aeruginosa
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.2.30 additional information the bacterial enzyme shows the ability to mediate cell death in the host and is a toxin, residues 426-428 are required for this activity Pseudomonas aeruginosa ?
-
 ?
2.4.2.30 NAD+ + (ADP-D-ribosyl)n-acceptor
-
 Pseudomonas aeruginosa nicotinamide + (ADP-D-ribosyl)n+1-acceptor
-
 ?
2.4.2.30 NAD+ + Ras interaction requires residue Leu428 Pseudomonas aeruginosa nicotinamide + (ADP-D-ribosyl)-Ras
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.4.2.30 ADP-ribosyltransferase
-
 Pseudomonas aeruginosa
2.4.2.30 exoenzyme S
-
 Pseudomonas aeruginosa
2.4.2.30 ExoS
-
 Pseudomonas aeruginosa