BRENDA - Enzyme Database

Spectroscopic characterization of a higher plant heme oxygenase isoform-1 from Glycine max (soybean)--coordination structure of the heme complex and catabolism of heme

Gohya, T.; Zhang, X.; Yoshida, T.; Migita, C.T.; FEBS J. 273, 5384-5399 (2006)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
1.14.14.18
expressed in Escherichia coli BL21 (DE3) cells
Glycine max
1.14.15.20
expressed in Escherichia coli BL21(DE3) cells
Glycine max
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.14.14.18
21600
-
calculated from amino acid sequence
Glycine max
1.14.14.18
26000
-
SDS-PAGE
Glycine max
1.14.15.20
26000
-
x * 26000, SDS-PAGE
Glycine max
1.14.15.20
26100
-
-
Glycine max
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.14.15.20
protoheme + reduced ferredoxin [iron-sulfur] cluster + O2 + H+
Glycine max
-
biliverdin IXalpha + Fe2+ + CO + oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.14.14.18
Glycine max
-
-
-
1.14.15.20
Glycine max
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
1.14.14.18
Sephadex G-75 gel filtration and DE-52 gel filtration
Glycine max
1.14.15.20
ammonium sulfate precipitation, DE-52 column chromatography, and Sephadex G-75 gel filtration
Glycine max
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.14.14.18
heme + NADPH + O2
-
673571
Glycine max
biliverdin IXalpha + Fe2+ + CO + NADP+ + H2O
-
-
-
?
1.14.15.20
protoheme + reduced ferredoxin [iron-sulfur] cluster + O2 + H+
-
673571
Glycine max
biliverdin IXalpha + Fe2+ + CO + oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
1.14.15.20
?
x * 26000, SDS-PAGE; x * 26100, calculated from amino acid sequence
Glycine max
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.14.14.18
heme
-
Glycine max
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
1.14.14.18
expressed in Escherichia coli BL21 (DE3) cells
Glycine max
1.14.15.20
expressed in Escherichia coli BL21(DE3) cells
Glycine max
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.14.14.18
heme
-
Glycine max
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.14.14.18
21600
-
calculated from amino acid sequence
Glycine max
1.14.14.18
26000
-
SDS-PAGE
Glycine max
1.14.15.20
26000
-
x * 26000, SDS-PAGE
Glycine max
1.14.15.20
26100
-
-
Glycine max
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.14.15.20
protoheme + reduced ferredoxin [iron-sulfur] cluster + O2 + H+
Glycine max
-
biliverdin IXalpha + Fe2+ + CO + oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.14.14.18
Sephadex G-75 gel filtration and DE-52 gel filtration
Glycine max
1.14.15.20
ammonium sulfate precipitation, DE-52 column chromatography, and Sephadex G-75 gel filtration
Glycine max
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.14.14.18
heme + NADPH + O2
-
673571
Glycine max
biliverdin IXalpha + Fe2+ + CO + NADP+ + H2O
-
-
-
?
1.14.15.20
protoheme + reduced ferredoxin [iron-sulfur] cluster + O2 + H+
-
673571
Glycine max
biliverdin IXalpha + Fe2+ + CO + oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
1.14.15.20
?
x * 26000, SDS-PAGE; x * 26100, calculated from amino acid sequence
Glycine max