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Literature summary extracted from
- Spectroscopic characterization of a higher plant heme oxygenase isoform-1 from Glycine max (soybean)--coordination structure of the heme complex and catabolism of heme (2006), FEBS J., 273, 5384-5399.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 1.14.14.18 | expressed in Escherichia coli BL21 (DE3) cells | Glycine max |
| 1.14.15.20 | expressed in Escherichia coli BL21(DE3) cells | Glycine max |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 1.14.14.18 | 21600 | - |
calculated from amino acid sequence | Glycine max |
| 1.14.14.18 | 26000 | - |
SDS-PAGE | Glycine max |
| 1.14.15.20 | 26000 | - |
x * 26000, SDS-PAGE | Glycine max |
| 1.14.15.20 | 26100 | - |
- |
Glycine max |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.14.15.20 | protoheme + reduced ferredoxin [iron-sulfur] cluster + O2 + H+ | Glycine max | - |
biliverdin IXalpha + Fe2+ + CO + oxidized ferredoxin [iron-sulfur] cluster + H2O | - |
? |  |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.14.14.18 | Glycine max | - |
- |
- |
| 1.14.15.20 | Glycine max | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.14.14.18 | Sephadex G-75 gel filtration and DE-52 gel filtration | Glycine max |
| 1.14.15.20 | ammonium sulfate precipitation, DE-52 column chromatography, and Sephadex G-75 gel filtration | Glycine max |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.14.14.18 | heme + NADPH + H+ + O2 | - |
Glycine max | biliverdin IXalpha + Fe2+ + CO + NADP+ + H2O | - |
? | |
| 1.14.15.20 | protoheme + reduced ferredoxin [iron-sulfur] cluster + O2 + H+ | - |
Glycine max | biliverdin IXalpha + Fe2+ + CO + oxidized ferredoxin [iron-sulfur] cluster + H2O | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 1.14.15.20 | ? | x * 26000, SDS-PAGE | Glycine max |
| 1.14.15.20 | ? | x * 26100, calculated from amino acid sequence | Glycine max |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 1.14.14.18 | HO-1 | - |
Glycine max |
| 1.14.15.20 | heme oxygenase | - |
Glycine max |
| 1.14.15.20 | HO-1 | - |
Glycine max |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.14.14.18 | heme | - |
Glycine max | |
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