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Literature summary extracted from

  • Hothi, P.; Abu Khadra, K.; Combe, J.P.; Leys, D.; Scrutton, N.S.
    Tryptophan tryptophylquinone cofactor biogenesis in the aromatic amine dehydrogenase of Alcaligenes faecalis. Cofactor assembly and catalytic properties of recombinant enzyme expressed in Paracoccus denitrificans. [Erratum to document cited in CA144:14530] (2006), FEBS J., 273, 430.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.4.9.2 additional information
-
 additional information analysis of limiting rate constants of reaction with benzylamine and deuterated benzylamine at different pH values Alcaligenes faecalis
1.4.9.2 6.7
-
 benzylamine pH 7.5, 25°C, native enzyme Alcaligenes faecalis
1.4.9.2 9.9
-
 benzylamine pH 7.5, 25°C, recombinant enzyme Alcaligenes faecalis

Organism

EC Number Organism UniProt Comment Textmining
1.4.9.2 Alcaligenes faecalis
-
 expression in Paracoccus denitrificans
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.4.9.2 recombinant enzyme, expression in Paracoccus denitrificans Alcaligenes faecalis

Reaction

EC Number Reaction Comment Organism Reaction ID
1.4.9.2 ArCH2NH2 + H2O + 2 azurin = ArCHO + NH3 + 2 reduced azurin breakage of the substrate C-H bond occurs via quantum mechanical tunneling. In steady-state reactions with benzylamine, breakage of C-H bond is only partially rate-limiting Alcaligenes faecalis
a

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.4.9.2 30.8
-
 pH 7.5, 25°C Alcaligenes faecalis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.4.9.2 benzylamine + H2O + 2,6-dichlorophenol analysis of limiting rate constants Alcaligenes faecalis ? + NH3 + reduced 2,6-dichlorophenol indophenol
-
 ?
1.4.9.2 phenazine ethosulfate + H2O + 2,6-dichlorophenol indophenol
-
 Alcaligenes faecalis ? + NH3 + reduced 2,6-dichlorophenol indophenol
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.4.9.2 1.02
-
 benzylamine pH 7.5, 25°C, recombinant enzyme Alcaligenes faecalis
1.4.9.2 1.14
-
 benzylamine pH 7.5, 25°C, native enzyme Alcaligenes faecalis