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Literature summary extracted from

  • Zameitat, E.; Gojkovic, Z.; Knecht, W.; Piskur, J.; Loeffler, M.
    Biochemical characterization of recombinant dihydroorotate dehydrogenase from the opportunistic pathogenic yeast Candida albicans (2006), FEBS J., 273, 3183-3191.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.3.5.2 oxygen seems to promote very low DHODH activity Candida albicans

Application

EC Number Application Comment Organism
1.3.5.2 medicine background for the development of antipyrimidines with high efficacy for decreasing in situ pyrimidine nucleotide pools in Candida albicans Candida albicans

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.3.5.2 full-length DHODH and N-terminally truncated DHODH, which lacks the targeting sequence and the transmembrane domain recombinantly expressed in Escherichia coli BL21 Candida albicans

Protein Variants

EC Number Protein Variants Comment Organism
1.3.5.2 L11S two CUG codons in the DHODH ORF changed to UCG by site-directed PCR mutagenesis for gene expression in the bacterial system Candida albicans
1.3.5.2 L78S two CUG codons in the DHODH ORF changed to UCG by site-directed PCR mutagenesis for gene expression in the bacterial system Candida albicans

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.3.5.2 brequinar sodium 1 mM reduces the activity by more than 50% of the full-length enzyme Candida albicans
1.3.5.2 redoxal 0.5 mM exhibit an inhibitory effect of more than 50% on full-length DHODH and N-terminally truncated DHODH compared with the noninhibited reaction Candida albicans

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.3.5.2 0.04
-
 dihydroorotate N-terminally truncated DHODH, fixed concentration of dihydroorotate and varied concentrations of 2,6-dichloroindophenol from 0-0.2 mM Candida albicans
1.3.5.2 0.042
-
 dihydroorotate full-length DHODH, fixed concentration of dihydroorotate of 1.0 mM and varied concentrations of decylubiquinone from 0-0.2 mM Candida albicans
1.3.5.2 0.065
-
 dihydroorotate N-terminally truncated DHODH, fixed concentration of dihydroorotate of 1.0 mM and varied concentrations of decylubiquinone from 0-0.2 mM Candida albicans
1.3.5.2 0.108
-
 dihydroorotate full-length DHODH, concentration of dihydroorotate varied from 0-1.0 mM at fixed concentrations of 0.1 mM decylubiquinone and 0.06 mM 2,6-dichloroindophenol Candida albicans
1.3.5.2 0.111
-
 dihydroorotate N-terminally truncated DHODH, concentration of dihydroorotate varied from 0-1.0 mM at fixed concentrations of 0.1 mM decylubiquinone and 0.06 mM 2,6-dichloroindophenol Candida albicans
1.3.5.2 0.122
-
 dihydroorotate full-length DHODH, fixed concentration of dihydroorotate of 1.0 mM and varied concentrations of 2,6-dichloroindophenol from 0-0.2 mM Candida albicans

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.3.5.2 48000
-
 48000 for the full-length enzyme and 42000 for the truncated enzyme, SDS-PAGE Candida albicans

Organism

EC Number Organism UniProt Comment Textmining
1.3.5.2 Candida albicans Q874I4
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.3.5.2 full-length DHODH and N-terminally truncated DHODH, by affinity chromatography Candida albicans

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.3.5.2 6
-
 using decylubiquinone and 2,6-dichloroindophenol as acceptor Candida albicans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.5.2 dihydroorotate + 2,3-dimethoxy-5-methyl-1,4-benzoquinone
-
 Candida albicans orotate + reduced 2,3-dimethoxy-5-methyl-1,4-benzoquinone
-
 ?
1.3.5.2 dihydroorotate + 2,6-dichloroindophenol
-
 Candida albicans orotate + reduced 2,6-dichloroindophenol
-
 ?
1.3.5.2 dihydroorotate + decylubiquinone
-
 Candida albicans orotate + reduced decylubiquinone
-
 ?
1.3.5.2 dihydroorotate + potassium hexacyanoferrate(III)
-
 Candida albicans orotate + reduced potassium hexacyanoferrate(III)
-
 ?
1.3.5.2 dihydroorotate + ubiquinone 30
-
 Candida albicans orotate + reduced ubiquinone 30
-
 ?
1.3.5.2 dihydroorotate + ubiquinone 50
-
 Candida albicans orotate + reduced ubiquinone 50
-
 ?
1.3.5.2 additional information fumarate and NAD are inadequate electron acceptors for full-length DHODH and N-terminally truncated DHODH Candida albicans ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.3.5.2 ? 48000 for the full-length enzyme and 42000 for the truncated enzyme, SDS-PAGE Candida albicans

Synonyms

EC Number Synonyms Comment Organism
1.3.5.2 DHODH
-
 Candida albicans

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.3.5.2 2.1
-
 dihydroorotate full-length DHODH Candida albicans
1.3.5.2 2.2
-
 dihydroorotate N-terminally truncated DHODH Candida albicans
1.3.99.11 2.1
-
 L-dihydroorotate
-
 Candida albicans

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.3.5.2 additional information
-
 8.0-8.5 Candida albicans

Cofactor

EC Number Cofactor Comment Organism Structure
1.3.5.2 flavin
-
 Candida albicans