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Literature summary extracted from

  • Siani, L.; Viggiani, A.; Notomista, E.; Pezzella, A.; Di Donato, A.
    The role of residue Thr249 in modulating the catalytic efficiency and substrate specificity of catechol-2,3-dioxygenase from Pseudomonas stutzeri OX1 (2006), FEBS J., 273, 2963-2976.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.13.11.2 expressed in Escherichia coli strain BL21(DE3) Pseudomonas stutzeri

Protein Variants

EC Number Protein Variants Comment Organism
1.13.11.2 T249A reduced kcat and increased Km-values Pseudomonas stutzeri
1.13.11.2 T249G reduced kcat and increased Km-values Pseudomonas stutzeri
1.13.11.2 T249S reduced kcat and increased Km-values Pseudomonas stutzeri

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.13.11.2 0.001
-
catechol wildtype Pseudomonas stutzeri
1.13.11.2 0.0038
-
3-methylcatechol wildtype Pseudomonas stutzeri
1.13.11.2 0.0055
-
3,5-dimethylcatechol mutant T249A Pseudomonas stutzeri
1.13.11.2 0.0074
-
3,5-dimethylcatechol mutant T249G Pseudomonas stutzeri
1.13.11.2 0.0097
-
3,5-dimethylcatechol mutant T249S Pseudomonas stutzeri
1.13.11.2 0.0115
-
3-methylcatechol mutant T249S Pseudomonas stutzeri
1.13.11.2 0.0143
-
3-methylcatechol mutant T249A Pseudomonas stutzeri
1.13.11.2 0.0215
-
3,5-dimethylcatechol wildtype Pseudomonas stutzeri
1.13.11.2 0.0225
-
catechol mutant T249S Pseudomonas stutzeri
1.13.11.2 0.0237
-
3,5-dimethylcatechol mutant T249G Pseudomonas stutzeri
1.13.11.2 0.0266
-
3-methylcatechol mutant T249G Pseudomonas stutzeri
1.13.11.2 0.037
-
catechol mutant T249A Pseudomonas stutzeri
1.13.11.2 0.0381
-
3,5-dimethylcatechol mutant T249A Pseudomonas stutzeri
1.13.11.2 0.0575
-
3,5-dimethylcatechol mutant T249S Pseudomonas stutzeri
1.13.11.2 0.0636
-
catechol mutant T249G Pseudomonas stutzeri
1.13.11.2 0.0738
-
3,5-dimethylcatechol wildtype Pseudomonas stutzeri

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.13.11.2 catechol + O2 Pseudomonas stutzeri
-
2-hydroxymuconate semialdehyde
-
?
1.13.11.2 catechol + O2 Pseudomonas stutzeri OX1
-
2-hydroxymuconate semialdehyde
-
?

Organism

EC Number Organism UniProt Comment Textmining
1.13.11.2 Pseudomonas stutzeri
-
OX1
-
1.13.11.2 Pseudomonas stutzeri OX1
-
OX1
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.13.11.2
-
Pseudomonas stutzeri

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.11.2 3,5-dimethylcatechol + O2
-
Pseudomonas stutzeri 2-hydroxy-3,5-dimethyl-6-oxohexa-2,4-dienoic acid
-
?
1.13.11.2 3,5-dimethylcatechol + O2
-
Pseudomonas stutzeri OX1 2-hydroxy-3,5-dimethyl-6-oxohexa-2,4-dienoic acid
-
?
1.13.11.2 3-methylcatechol + O2
-
Pseudomonas stutzeri 2-hydroxy-6-oxohepta-2,4-dienoic acid
-
?
1.13.11.2 3-methylcatechol + O2
-
Pseudomonas stutzeri OX1 2-hydroxy-6-oxohepta-2,4-dienoic acid
-
?
1.13.11.2 catechol + O2
-
Pseudomonas stutzeri 2-hydroxymuconate semialdehyde
-
?
1.13.11.2 catechol + O2
-
Pseudomonas stutzeri OX1 2-hydroxymuconate semialdehyde
-
?

Synonyms

EC Number Synonyms Comment Organism
1.13.11.2 C2,3O
-
Pseudomonas stutzeri

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.13.11.2 0.23
-
3,5-dimethylcatechol mutant T249G Pseudomonas stutzeri
1.13.11.2 0.36
-
3,5-dimethylcatechol wildtype Pseudomonas stutzeri
1.13.11.2 0.4
-
3,5-dimethylcatechol mutant T249A Pseudomonas stutzeri
1.13.11.2 0.4
-
3,5-dimethylcatechol mutant T249G Pseudomonas stutzeri
1.13.11.2 0.66
-
3,5-dimethylcatechol wildtype Pseudomonas stutzeri
1.13.11.2 0.8
-
catechol mutant T249G Pseudomonas stutzeri
1.13.11.2 1
-
3,5-dimethylcatechol mutant T249A Pseudomonas stutzeri
1.13.11.2 1.2
-
3,5-dimethylcatechol mutant T249S Pseudomonas stutzeri
1.13.11.2 1.3
-
catechol mutant T249A Pseudomonas stutzeri
1.13.11.2 2.6
-
3-methylcatechol mutant T249G Pseudomonas stutzeri
1.13.11.2 2.65
-
3,5-dimethylcatechol mutant T249S Pseudomonas stutzeri
1.13.11.2 6.3
-
3-methylcatechol mutant T249A Pseudomonas stutzeri
1.13.11.2 7.5
-
catechol mutant T249S Pseudomonas stutzeri
1.13.11.2 60.5
-
3-methylcatechol mutant T249S Pseudomonas stutzeri
1.13.11.2 118
-
3-methylcatechol wildtype Pseudomonas stutzeri
1.13.11.2 180
-
catechol wildtype Pseudomonas stutzeri