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Literature summary extracted from

  • Forzi, L.; Koch, J.; Guss, A.M.; Radosevich, C.G.; Metcalf, W.W.; Hedderich, R.
    Assignment of the [4Fe-4S] clusters of Ech hydrogenase from Methanosarcina barkeri to individual subunits via the characterization of site-directed mutants (2005), FEBS J., 272, 4741-4753.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
1.12.7.2 C42S mutant enzyme with almost no activity in the reaction with H2 and oxidized benzyl viologen Methanosarcina barkeri
1.12.7.2 C45S mutants with about 5% of the wild-type activity in the reaction with H2 and oxidized benzyl viologen Methanosarcina barkeri
1.12.7.2 C48S mutant enzyme with almost no activity in the reaction with H2 and oxidized benzyl viologen Methanosarcina barkeri
1.12.7.2 C73S mutants with about 5% of the wild-type activity in the reaction with H2 and oxidized benzyl viologen Methanosarcina barkeri
1.12.7.2 C76S mutant enzyme with almost no activity in the reaction with H2 and oxidized benzyl viologen Methanosarcina barkeri
1.12.7.2 C79S mutants with about 5% of the wild-type activity in the reaction with H2 and oxidized benzyl viologen Methanosarcina barkeri
1.12.7.2 C83S mutant enzyme with approximately 10% of the activity of the wild-type enzyme in the reaction with H2 and oxidized benzyl viologen Methanosarcina barkeri

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.12.7.2 membrane bound to Methanosarcina barkeri 16020
-

Organism

EC Number Organism UniProt Comment Textmining
1.12.7.2 Methanosarcina barkeri
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.12.7.2
-
 Methanosarcina barkeri

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.12.7.2 H2 + ferredoxin + oxidized metronidazole
-
 Methanosarcina barkeri H+ + ferredoxin + reduced metronidazole
-
 ?
1.12.7.2 H2 + oxidized benzyl viologen wild-type enzyme catalysed the reduction of benzylviologen at fourfold higher rates than the reduction of ferredoxin Methanosarcina barkeri reduced benzyl viologen + H+
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.12.7.2 Ech hydrogenase
-
 Methanosarcina barkeri

Cofactor

EC Number Cofactor Comment Organism Structure
1.12.7.2 iron-sulfur centre the three [4Fe-4S] clusters simultaneously mediate electron and proton transfer and thus could be an essential part of the proton-translocating machinery Methanosarcina barkeri