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Literature summary extracted from
- Electron transfer chain reaction of the extracellular flavocytochrome cellobiose dehydrogenase from the basidiomycete Phanerochaete chrysosporium (2005), FEBS J., 272, 2869-2877.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 1.1.99.18 | wild type and mutant cellobiose dehydrogenase genes are cloned for heterologously expression in yeast Pichia pastoris | Phanerodontia chrysosporium |
Protein Variants
| EC Number | Protein Variants | Comment | Organism |
|---|---|---|---|
| 1.1.99.18 | F166Y | the redox potential of heme in the mutant is lower than that of the wild type enzyme | Phanerodontia chrysosporium |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.1.99.18 | cytochrome c | substrate inhibition | Phanerodontia chrysosporium |  |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.1.99.18 | 0.00067 | - |
cytochrome c | mutant F166Y, cytochrome c reduction | Phanerodontia chrysosporium | |
| 1.1.99.18 | 0.00146 | - |
cytochrome c | wild type, cytochrome c reduction | Phanerodontia chrysosporium | |
| 1.1.99.18 | 0.0179 | - |
cytochrome c | mutant F166Y, cellobiose oxidation with electron acceptors | Phanerodontia chrysosporium | |
| 1.1.99.18 | 0.0286 | - |
cytochrome c | wild type, cellobiose oxidation with electron acceptors | Phanerodontia chrysosporium | |
| 1.1.99.18 | 0.056 | - |
ubiquinone | mutant F166Y, cellobiose oxidation with electron acceptors | Phanerodontia chrysosporium | |
| 1.1.99.18 | 0.0579 | - |
ubiquinone | wild type, cellobiose oxidation with electron acceptors | Phanerodontia chrysosporium | |
| 1.1.99.18 | 0.293 | - |
ubiquinone | mutant F166Y, ubiquinone reduction | Phanerodontia chrysosporium | |
| 1.1.99.18 | 0.326 | - |
ubiquinone | wild type, ubiquinone reduction | Phanerodontia chrysosporium | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 1.1.99.18 | extracellular | - |
Phanerodontia chrysosporium | - |
- |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.1.99.18 | cellobiose + acceptor | Phanerodontia chrysosporium | acceptor: cytochrome c | cellobiono-1,5-lactone + reduced acceptor | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.1.99.18 | Phanerodontia chrysosporium | Q01738 | - |
- |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.1.99.18 | cellobiose + acceptor | acceptor: cytochrome c | Phanerodontia chrysosporium | cellobiono-1,5-lactone + reduced acceptor | - |
? | |
| 1.1.99.18 | cellobiose + ubiquinone | - |
Phanerodontia chrysosporium | cellobiono-1,5-lactone + reduced ubiquinone | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 1.1.99.18 | Cdh | - |
Phanerodontia chrysosporium |
| 1.1.99.18 | cellobiose dehydrogenase | - |
Phanerodontia chrysosporium |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.1.99.18 | 30 | - |
activity assay | Phanerodontia chrysosporium |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.1.99.18 | 4 | - |
activity assay | Phanerodontia chrysosporium |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.1.99.18 | flavin | - |
Phanerodontia chrysosporium | |
| 1.1.99.18 | heme | b-type | Phanerodontia chrysosporium | |
Ki Value [mM]
| EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.1.99.18 | 0.233 | - |
cytochrome c | mutant F166Y, cytochrome c reduction | Phanerodontia chrysosporium | |
| 1.1.99.18 | 2.51 | - |
cytochrome c | wild type, cellobiose oxidation with electron acceptors | Phanerodontia chrysosporium | |
| 1.1.99.18 | 3.54 | - |
cytochrome c | mutant F166Y, cellobiose oxidation with electron acceptors | Phanerodontia chrysosporium | |
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Release 2023.1 (January 2023)
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