Any feedback?
Literature summary extracted from
- Divergent cyclooxygenase responses to fatty acid structure and peroxide level in fish and mammalian prostaglandin H synthases (2006), FASEB J., 20, 1097-1108.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 1.14.99.1 | - |
Ovis aries |
| 1.14.99.1 | - |
Homo sapiens |
| 1.14.99.1 | expression in Spodoptera frugiperda Sf-9 cells | Salvelinus fontinalis |
| 1.14.99.1 | into a pVL1392 vector for transformation of Escherichia coli DH5alpha competent cells | Salvelinus fontinalis |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.14.99.1 | 0.0011 | - |
cis-4,7,10,13,16,19-docosahexaenoic acid | - |
Homo sapiens |  |
| 1.14.99.1 | 0.0012 | - |
cis-5,8,11,14,17-eicosapentaenoic acid | - |
Homo sapiens | |
| 1.14.99.1 | 0.0017 | - |
cis-5,8,11,14-eicosatetraenoic acid | - |
Homo sapiens | |
| 1.14.99.1 | 0.002 | - |
cis-8,11,14-eicosatrienoic acid | - |
Homo sapiens | |
| 1.14.99.1 | 0.0024 | - |
cis-5,8,11,14-eicosatetraenoic acid | - |
Salvelinus fontinalis | |
| 1.14.99.1 | 0.0027 | - |
cis-7,10,13,16-docosatetraenoic acid | - |
Homo sapiens | |
| 1.14.99.1 | 0.003 | - |
cis-5,8,11,14-eicosatetraenoic acid | - |
Ovis aries | |
| 1.14.99.1 | 0.0031 | - |
alpha-linolenic acid | - |
Homo sapiens | |
| 1.14.99.1 | 0.0031 | - |
cis-5,8,11,14,17-eicosapentaenoic acid | - |
Ovis aries | |
| 1.14.99.1 | 0.0033 | - |
cis-4,7,10,13,16,19-docosahexaenoic acid | - |
Ovis aries | |
| 1.14.99.1 | 0.0048 | - |
alpha-linolenic acid | - |
Ovis aries | |
| 1.14.99.1 | 0.0048 | - |
gamma-linolenic acid | - |
Homo sapiens | |
| 1.14.99.1 | 0.0052 | - |
cis-11,14-eicosadienoic acid | - |
Homo sapiens | |
| 1.14.99.1 | 0.0055 | - |
cis-8,11,14-eicosatrienoic acid | - |
Ovis aries | |
| 1.14.99.1 | 0.0059 | - |
cis-8,11,14-eicosatrienoic acid | - |
Salvelinus fontinalis | |
| 1.14.99.1 | 0.007 | - |
cis-7,10,13,16-docosatetraenoic acid | - |
Ovis aries | |
| 1.14.99.1 | 0.0071 | - |
gamma-linolenic acid | - |
Ovis aries | |
| 1.14.99.1 | 0.0091 | - |
cis-11,14-eicosadienoic acid | - |
Ovis aries | |
| 1.14.99.1 | 0.0101 | - |
alpha-linolenic acid | - |
Salvelinus fontinalis | |
| 1.14.99.1 | 0.013 | - |
cis-5,8,11,14-eicosatetraenoic acid | - |
Salvelinus fontinalis | |
| 1.14.99.1 | 0.0153 | - |
cis-5,8,11,14,17-eicosapentaenoic acid | - |
Salvelinus fontinalis | |
| 1.14.99.1 | 0.0318 | - |
cis-4,7,10,13,16,19-docosahexaenoic acid | - |
Salvelinus fontinalis | |
| 1.14.99.1 | 0.0357 | - |
cis-7,10,13,16-docosatetraenoic acid | - |
Salvelinus fontinalis | |
| 1.14.99.1 | 0.036 | - |
cis-8,11,14-eicosatrienoic acid | - |
Salvelinus fontinalis | |
| 1.14.99.1 | 0.039 | - |
cis-5,8,11,14,17-eicosapentaenoic acid | - |
Salvelinus fontinalis | |
| 1.14.99.1 | 0.061 | - |
cis-7,10,13,16-docosatetraenoic acid | - |
Salvelinus fontinalis | |
| 1.14.99.1 | 0.07 | - |
cis-4,7,10,13,16,19-docosahexaenoic acid | - |
Salvelinus fontinalis | |
| 1.14.99.1 | 0.082 | - |
alpha-linolenic acid | - |
Salvelinus fontinalis | |
| 1.14.99.1 | 0.162 | - |
gamma-linolenic acid | - |
Salvelinus fontinalis | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 1.14.99.1 | membrane | - |
Salvelinus fontinalis | 16020 | - |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.14.99.1 | arachidonate + AH2 + O2 | Ovis aries | - |
prostaglandin H2 + A + H2O | - |
? | |
| 1.14.99.1 | arachidonate + AH2 + O2 | Homo sapiens | - |
prostaglandin H2 + A + H2O | - |
? | |
| 1.14.99.1 | arachidonate + AH2 + O2 | Salvelinus fontinalis | - |
prostaglandin H2 + A + H2O | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.14.99.1 | Homo sapiens | P23219 | PGHS-1 | - |
| 1.14.99.1 | Homo sapiens | P35354 | PGHS-2 | - |
| 1.14.99.1 | Ovis aries | - |
- |
- |
| 1.14.99.1 | Salvelinus fontinalis | Q9PTN3 | PGHS-1 | - |
| 1.14.99.1 | Salvelinus fontinalis | Q9PW89 | PGHS-2 | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.14.99.1 | - |
Ovis aries |
| 1.14.99.1 | recombinant protein expressed in Sf9 cells | Homo sapiens |
| 1.14.99.1 | recombinant protein expressed in Sf9 cells, protein is purified from the membrane fraction using a High Q ion exchange column | Salvelinus fontinalis |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 1.14.99.1 | seminal vesicle | - |
Ovis aries | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 1.14.99.1 | 2300 | - |
cyclooxygenase activity for partially purified prostaglandin H synthase 1 of Salvelinus fontinalis, tPGHS-1 | Salvelinus fontinalis |
| 1.14.99.1 | 15000 | - |
cyclooxygenase activity for partially purified prostaglandin H synthase 2 of Salvelinus fontinalis, tPGHS-2 | Salvelinus fontinalis |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.14.99.1 | (4Z,7Z,10Z,13Z,16Z,19Z)-docosahexaenoic acid + AH2 + O2 | - |
Ovis aries | ? + A + H2O | - |
? | |
| 1.14.99.1 | (4Z,7Z,10Z,13Z,16Z,19Z)-docosahexaenoic acid + AH2 + O2 | - |
Salvelinus fontinalis | ? + A + H2O | - |
? | |
| 1.14.99.1 | alpha-linolenic acid + reduced acceptor + O2 | - |
Ovis aries | ? | - |
r | |
| 1.14.99.1 | alpha-linolenic acid + reduced acceptor + O2 | - |
Salvelinus fontinalis | ? | - |
r | |
| 1.14.99.1 | arachidonate + AH2 + O2 | - |
Ovis aries | prostaglandin H2 + A + H2O | - |
? | |
| 1.14.99.1 | arachidonate + AH2 + O2 | - |
Homo sapiens | prostaglandin H2 + A + H2O | - |
? | |
| 1.14.99.1 | arachidonate + AH2 + O2 | - |
Salvelinus fontinalis | prostaglandin H2 + A + H2O | - |
? | |
| 1.14.99.1 | cis-11,14-eicosadienoic acid + AH2 + O2 | - |
Homo sapiens | ? | - |
? | |
| 1.14.99.1 | cis-11,14-eicosadienoic acid + reduced acceptor + O2 | - |
Ovis aries | ? | - |
r | |
| 1.14.99.1 | cis-4,7,10,13,16,19-docosahexaenoic acid + AH2 + O2 | - |
Homo sapiens | ? | - |
? | |
| 1.14.99.1 | cis-5,8,11,14,17-eicosapentaenoic acid + AH2 + O2 | - |
Homo sapiens | ? | - |
? | |
| 1.14.99.1 | cis-5,8,11,14,17-eicosapentaenoic acid + reduced acceptor + O2 | - |
Ovis aries | ? | - |
r | |
| 1.14.99.1 | cis-5,8,11,14,17-eicosapentaenoic acid + reduced acceptor + O2 | - |
Salvelinus fontinalis | ? | - |
r | |
| 1.14.99.1 | cis-5,8,11,14-eicosatetraenoic acid + AH2 + O2 | - |
Homo sapiens | ? | - |
? | |
| 1.14.99.1 | cis-7,10,13,16-docosatetraenoic acid + AH2 + O2 | - |
Homo sapiens | ? | - |
? | |
| 1.14.99.1 | cis-7,10,13,16-docosatetraenoic acid + reduced acceptor + O2 | - |
Ovis aries | ? | - |
r | |
| 1.14.99.1 | cis-7,10,13,16-docosatetraenoic acid + reduced acceptor + O2 | - |
Salvelinus fontinalis | ? | - |
r | |
| 1.14.99.1 | cis-8,11,14-eicosatrienoic acid + AH2 + O2 | - |
Homo sapiens | ? | - |
? | |
| 1.14.99.1 | cis-8,11,14-eicosatrienoic acid + reduced acceptor + O2 | - |
Ovis aries | ? | - |
r | |
| 1.14.99.1 | cis-8,11,14-eicosatrienoic acid + reduced acceptor + O2 | - |
Salvelinus fontinalis | ? | - |
r | |
| 1.14.99.1 | gamma-linolenic acid + AH2 + O2 | - |
Homo sapiens | ? | - |
? | |
| 1.14.99.1 | gamma-linolenic acid + reduced acceptor + O2 | - |
Ovis aries | ? | - |
r | |
| 1.14.99.1 | gamma-linolenic acid + reduced acceptor + O2 | - |
Salvelinus fontinalis | ? | - |
r | |
| 1.14.99.1 | linolenic acid + AH2 + O2 | - |
Homo sapiens | ? | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 1.14.99.1 | hPGHS-1 | - |
Homo sapiens |
| 1.14.99.1 | hPGHS-2 | - |
Homo sapiens |
| 1.14.99.1 | oPGHS-1 | - |
Ovis aries |
| 1.14.99.1 | PGHS | - |
Ovis aries |
| 1.14.99.1 | PGHS | - |
Homo sapiens |
| 1.14.99.1 | PGHS | - |
Salvelinus fontinalis |
| 1.14.99.1 | PGHS-1 | - |
Ovis aries |
| 1.14.99.1 | PGHS-1 | - |
Homo sapiens |
| 1.14.99.1 | PGHS-1 | - |
Salvelinus fontinalis |
| 1.14.99.1 | PGHS-2 | - |
Homo sapiens |
| 1.14.99.1 | PGHS-2 | - |
Salvelinus fontinalis |
| 1.14.99.1 | prostaglandin H synthase | - |
Ovis aries |
| 1.14.99.1 | prostaglandin H synthase | - |
Homo sapiens |
| 1.14.99.1 | prostaglandin H synthase | - |
Salvelinus fontinalis |
| 1.14.99.1 | tPGHS-1 | brook trout, Salvelinus fontinalis | Salvelinus fontinalis |
| 1.14.99.1 | tPGHS-2 | brook trout, Salvelinus fontinalis | Salvelinus fontinalis |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.14.99.1 | 23 | - |
room temperature, 22-23°C, lipoxygenase activity assay | Homo sapiens |
| 1.14.99.1 | 23 | - |
room temperature, 22-23°C, lipoxygenase activity assay | Salvelinus fontinalis |
| 1.14.99.1 | 25 | - |
cyclooxygenase activity assay using a standard electrode | Ovis aries |
| 1.14.99.1 | 25 | - |
cyclooxygenase activity assay using a standard electrode | Homo sapiens |
| 1.14.99.1 | 25 | - |
cyclooxygenase activity assay using a standard electrode | Salvelinus fontinalis |
| 1.14.99.1 | 30 | - |
cyclooxygenase activity assay using a high sensitivity electrode | Ovis aries |
| 1.14.99.1 | 30 | - |
cyclooxygenase activity assay using a high sensitivity electrode | Homo sapiens |
| 1.14.99.1 | 30 | - |
cyclooxygenase activity assay using a high sensitivity electrode | Salvelinus fontinalis |
Temperature Range [°C]
| EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.14.99.1 | 5 | 30 | - |
Ovis aries |
| 1.14.99.1 | 5 | 30 | - |
Homo sapiens |
| 1.14.99.1 | 5 | 30 | - |
Salvelinus fontinalis |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.14.99.1 | 7.2 | - |
cyclooxygenase activity assay | Ovis aries |
| 1.14.99.1 | 7.2 | - |
cyclooxygenase activity assay | Homo sapiens |
| 1.14.99.1 | 7.2 | - |
cyclooxygenase activity assay | Salvelinus fontinalis |
| 1.14.99.1 | 8 | - |
lipoxygenase activity assay | Homo sapiens |
| 1.14.99.1 | 8 | - |
lipoxygenase activity assay | Salvelinus fontinalis |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
