BRENDA - Enzyme Database show

Divergent cyclooxygenase responses to fatty acid structure and peroxide level in fish and mammalian prostaglandin H synthases

Liu, W.; Cao, D.; Oh, S.F.; Serhan, C.N.; Kulmacz, R.J.; FASEB J. 20, 1097-1108 (2006)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
1.14.99.1
-
Ovis aries
1.14.99.1
;
Homo sapiens
1.14.99.1
expression in Spodoptera frugiperda Sf-9 cells; expression in Spodoptera frugiperda Sf-9 cells; into a pVL1392 vector for transformation of Escherichia coli DH5alpha competent cells; into a pVL1392 vector for transformation of Escherichia coli DH5alpha competent cells
Salvelinus fontinalis
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.14.99.1
0.0011
-
cis-4,7,10,13,16,19-docosahexaenoic acid
-
Homo sapiens
1.14.99.1
0.0012
-
cis-5,8,11,14,17-eicosapentaenoic acid
-
Homo sapiens
1.14.99.1
0.0017
-
cis-5,8,11,14-eicosatetraenoic acid
-
Homo sapiens
1.14.99.1
0.002
-
cis-8,11,14-eicosatrienoic acid
-
Homo sapiens
1.14.99.1
0.0024
-
cis-5,8,11,14-eicosatetraenoic acid
-
Salvelinus fontinalis
1.14.99.1
0.0027
-
cis-7,10,13,16-docosatetraenoic acid
-
Homo sapiens
1.14.99.1
0.003
-
cis-5,8,11,14-eicosatetraenoic acid
-
Ovis aries
1.14.99.1
0.0031
-
alpha-linolenic acid
-
Homo sapiens
1.14.99.1
0.0031
-
cis-5,8,11,14,17-eicosapentaenoic acid
-
Ovis aries
1.14.99.1
0.0033
-
cis-4,7,10,13,16,19-docosahexaenoic acid
-
Ovis aries
1.14.99.1
0.0048
-
alpha-linolenic acid
-
Ovis aries
1.14.99.1
0.0048
-
gamma-linolenic acid
-
Homo sapiens
1.14.99.1
0.0052
-
cis-11,14-eicosadienoic acid
-
Homo sapiens
1.14.99.1
0.0055
-
cis-8,11,14-eicosatrienoic acid
-
Ovis aries
1.14.99.1
0.0059
-
cis-8,11,14-eicosatrienoic acid
-
Salvelinus fontinalis
1.14.99.1
0.007
-
cis-7,10,13,16-docosatetraenoic acid
-
Ovis aries
1.14.99.1
0.0071
-
gamma-linolenic acid
-
Ovis aries
1.14.99.1
0.0091
-
cis-11,14-eicosadienoic acid
-
Ovis aries
1.14.99.1
0.0101
-
alpha-linolenic acid
-
Salvelinus fontinalis
1.14.99.1
0.013
-
cis-5,8,11,14-eicosatetraenoic acid
-
Salvelinus fontinalis
1.14.99.1
0.0153
-
cis-5,8,11,14,17-eicosapentaenoic acid
-
Salvelinus fontinalis
1.14.99.1
0.0318
-
cis-4,7,10,13,16,19-docosahexaenoic acid
-
Salvelinus fontinalis
1.14.99.1
0.0357
-
cis-7,10,13,16-docosatetraenoic acid
-
Salvelinus fontinalis
1.14.99.1
0.036
-
cis-8,11,14-eicosatrienoic acid
-
Salvelinus fontinalis
1.14.99.1
0.039
-
cis-5,8,11,14,17-eicosapentaenoic acid
-
Salvelinus fontinalis
1.14.99.1
0.061
-
cis-7,10,13,16-docosatetraenoic acid
-
Salvelinus fontinalis
1.14.99.1
0.07
-
cis-4,7,10,13,16,19-docosahexaenoic acid
-
Salvelinus fontinalis
1.14.99.1
0.082
-
alpha-linolenic acid
-
Salvelinus fontinalis
1.14.99.1
0.162
-
gamma-linolenic acid
-
Salvelinus fontinalis
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
1.14.99.1
membrane
;
Salvelinus fontinalis
16020
-
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.14.99.1
arachidonate + AH2 + O2
Ovis aries
-
prostaglandin H2 + A + H2O
-
-
?
1.14.99.1
arachidonate + AH2 + O2
Homo sapiens
-
prostaglandin H2 + A + H2O
-
-
?
1.14.99.1
arachidonate + AH2 + O2
Salvelinus fontinalis
-
prostaglandin H2 + A + H2O
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.14.99.1
Homo sapiens
P23219
PGHS-1
-
1.14.99.1
Homo sapiens
P35354
PGHS-2
-
1.14.99.1
Ovis aries
-
-
-
1.14.99.1
Salvelinus fontinalis
Q9PTN3
PGHS-1
-
1.14.99.1
Salvelinus fontinalis
Q9PW89
PGHS-2
-
Purification (Commentary)
EC Number
Commentary
Organism
1.14.99.1
-
Ovis aries
1.14.99.1
recombinant protein expressed in Sf9 cells, protein is purified from the membrane fraction using a High Q ion exchange column; recombinant protein expressed in Sf9 cells, protein is purified from the membrane fraction using a High Q ion exchange column
Salvelinus fontinalis
1.14.99.1
recombinant protein expressed in Sf9 cells; recombinant protein expressed in Sf9 cells
Homo sapiens
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
1.14.99.1
seminal vesicle
-
Ovis aries
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
1.14.99.1
2300
-
cyclooxygenase activity for partially purified prostaglandin H synthase 1 of Salvelinus fontinalis, tPGHS-1
Salvelinus fontinalis
1.14.99.1
15000
-
cyclooxygenase activity for partially purified prostaglandin H synthase 2 of Salvelinus fontinalis, tPGHS-2
Salvelinus fontinalis
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.14.99.1
(4Z,7Z,10Z,13Z,16Z,19Z)-docosahexaenoic acid + AH2 + O2
-
673508
Ovis aries
? + A + H2O
-
-
-
-
1.14.99.1
(4Z,7Z,10Z,13Z,16Z,19Z)-docosahexaenoic acid + AH2 + O2
-
673508
Salvelinus fontinalis
? + A + H2O
-
-
-
-
1.14.99.1
arachidonate + AH2 + O2
-
673508
Ovis aries
prostaglandin H2 + A + H2O
-
-
-
?
1.14.99.1
arachidonate + AH2 + O2
-
673508
Homo sapiens
prostaglandin H2 + A + H2O
-
-
-
?
1.14.99.1
arachidonate + AH2 + O2
-
673508
Salvelinus fontinalis
prostaglandin H2 + A + H2O
-
-
-
?
1.14.99.1
cis-11,14-eicosadienoic acid + AH2 + O2
-
673508
Homo sapiens
?
-
-
-
?
1.14.99.1
cis-4,7,10,13,16,19-docosahexaenoic acid + AH2 + O2
-
673508
Homo sapiens
?
-
-
-
?
1.14.99.1
cis-5,8,11,14,17-eicosapentaenoic acid + AH2 + O2
-
673508
Homo sapiens
?
-
-
-
?
1.14.99.1
cis-5,8,11,14-eicosatetraenoic acid + AH2 + O2
-
673508
Homo sapiens
?
-
-
-
?
1.14.99.1
cis-7,10,13,16-docosatetraenoic acid + AH2 + O2
-
673508
Homo sapiens
?
-
-
-
?
1.14.99.1
cis-8,11,14-eicosatrienoic acid + AH2 + O2
-
673508
Homo sapiens
?
-
-
-
?
1.14.99.1
gamma-linolenic acid + AH2 + O2
-
673508
Homo sapiens
?
-
-
-
?
1.14.99.1
linolenic acid + AH2 + O2
-
673508
Homo sapiens
?
-
-
-
?
Temperature Optimum [C]
EC Number
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
1.14.99.1
23
-
room temperature, 22-23C, lipoxygenase activity assay; room temperature, 22-23C, lipoxygenase activity assay
Homo sapiens
1.14.99.1
23
-
room temperature, 22-23C, lipoxygenase activity assay; room temperature, 22-23C, lipoxygenase activity assay
Salvelinus fontinalis
1.14.99.1
25
-
cyclooxygenase activity assay using a standard electrode; cyclooxygenase activity assay using a standard electrode
Homo sapiens
1.14.99.1
25
-
cyclooxygenase activity assay using a standard electrode
Ovis aries
1.14.99.1
25
-
cyclooxygenase activity assay using a standard electrode; cyclooxygenase activity assay using a standard electrode
Salvelinus fontinalis
1.14.99.1
30
-
cyclooxygenase activity assay using a high sensitivity electrode; cyclooxygenase activity assay using a high sensitivity electrode
Homo sapiens
1.14.99.1
30
-
cyclooxygenase activity assay using a high sensitivity electrode
Ovis aries
1.14.99.1
30
-
cyclooxygenase activity assay using a high sensitivity electrode; cyclooxygenase activity assay using a high sensitivity electrode
Salvelinus fontinalis
Temperature Range [C]
EC Number
Temperature Minimum [C]
Temperature Maximum [C]
Commentary
Organism
1.14.99.1
5
30
;
Homo sapiens
1.14.99.1
5
30
-
Ovis aries
1.14.99.1
5
30
;
Salvelinus fontinalis
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.14.99.1
7.2
-
cyclooxygenase activity assay; cyclooxygenase activity assay
Homo sapiens
1.14.99.1
7.2
-
cyclooxygenase activity assay
Ovis aries
1.14.99.1
7.2
-
cyclooxygenase activity assay; cyclooxygenase activity assay
Salvelinus fontinalis
1.14.99.1
8
-
lipoxygenase activity assay; lipoxygenase activity assay
Homo sapiens
1.14.99.1
8
-
lipoxygenase activity assay; lipoxygenase activity assay
Salvelinus fontinalis
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
1.14.99.1
-
Homo sapiens
1.14.99.1
expression in Spodoptera frugiperda Sf-9 cells; into a pVL1392 vector for transformation of Escherichia coli DH5alpha competent cells
Salvelinus fontinalis
1.14.99.1
-
Ovis aries
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.14.99.1
0.0011
-
cis-4,7,10,13,16,19-docosahexaenoic acid
-
Homo sapiens
1.14.99.1
0.0012
-
cis-5,8,11,14,17-eicosapentaenoic acid
-
Homo sapiens
1.14.99.1
0.0017
-
cis-5,8,11,14-eicosatetraenoic acid
-
Homo sapiens
1.14.99.1
0.002
-
cis-8,11,14-eicosatrienoic acid
-
Homo sapiens
1.14.99.1
0.0024
-
cis-5,8,11,14-eicosatetraenoic acid
-
Salvelinus fontinalis
1.14.99.1
0.0027
-
cis-7,10,13,16-docosatetraenoic acid
-
Homo sapiens
1.14.99.1
0.003
-
cis-5,8,11,14-eicosatetraenoic acid
-
Ovis aries
1.14.99.1
0.0031
-
alpha-linolenic acid
-
Homo sapiens
1.14.99.1
0.0031
-
cis-5,8,11,14,17-eicosapentaenoic acid
-
Ovis aries
1.14.99.1
0.0033
-
cis-4,7,10,13,16,19-docosahexaenoic acid
-
Ovis aries
1.14.99.1
0.0048
-
alpha-linolenic acid
-
Ovis aries
1.14.99.1
0.0048
-
gamma-linolenic acid
-
Homo sapiens
1.14.99.1
0.0052
-
cis-11,14-eicosadienoic acid
-
Homo sapiens
1.14.99.1
0.0055
-
cis-8,11,14-eicosatrienoic acid
-
Ovis aries
1.14.99.1
0.0059
-
cis-8,11,14-eicosatrienoic acid
-
Salvelinus fontinalis
1.14.99.1
0.007
-
cis-7,10,13,16-docosatetraenoic acid
-
Ovis aries
1.14.99.1
0.0071
-
gamma-linolenic acid
-
Ovis aries
1.14.99.1
0.0091
-
cis-11,14-eicosadienoic acid
-
Ovis aries
1.14.99.1
0.0101
-
alpha-linolenic acid
-
Salvelinus fontinalis
1.14.99.1
0.013
-
cis-5,8,11,14-eicosatetraenoic acid
-
Salvelinus fontinalis
1.14.99.1
0.0153
-
cis-5,8,11,14,17-eicosapentaenoic acid
-
Salvelinus fontinalis
1.14.99.1
0.0318
-
cis-4,7,10,13,16,19-docosahexaenoic acid
-
Salvelinus fontinalis
1.14.99.1
0.0357
-
cis-7,10,13,16-docosatetraenoic acid
-
Salvelinus fontinalis
1.14.99.1
0.036
-
cis-8,11,14-eicosatrienoic acid
-
Salvelinus fontinalis
1.14.99.1
0.039
-
cis-5,8,11,14,17-eicosapentaenoic acid
-
Salvelinus fontinalis
1.14.99.1
0.061
-
cis-7,10,13,16-docosatetraenoic acid
-
Salvelinus fontinalis
1.14.99.1
0.07
-
cis-4,7,10,13,16,19-docosahexaenoic acid
-
Salvelinus fontinalis
1.14.99.1
0.082
-
alpha-linolenic acid
-
Salvelinus fontinalis
1.14.99.1
0.162
-
gamma-linolenic acid
-
Salvelinus fontinalis
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
1.14.99.1
membrane
-
Salvelinus fontinalis
16020
-
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.14.99.1
arachidonate + AH2 + O2
Ovis aries
-
prostaglandin H2 + A + H2O
-
-
?
1.14.99.1
arachidonate + AH2 + O2
Homo sapiens
-
prostaglandin H2 + A + H2O
-
-
?
1.14.99.1
arachidonate + AH2 + O2
Salvelinus fontinalis
-
prostaglandin H2 + A + H2O
-
-
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.14.99.1
-
Ovis aries
1.14.99.1
recombinant protein expressed in Sf9 cells
Homo sapiens
1.14.99.1
recombinant protein expressed in Sf9 cells, protein is purified from the membrane fraction using a High Q ion exchange column
Salvelinus fontinalis
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
1.14.99.1
seminal vesicle
-
Ovis aries
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
1.14.99.1
2300
-
cyclooxygenase activity for partially purified prostaglandin H synthase 1 of Salvelinus fontinalis, tPGHS-1
Salvelinus fontinalis
1.14.99.1
15000
-
cyclooxygenase activity for partially purified prostaglandin H synthase 2 of Salvelinus fontinalis, tPGHS-2
Salvelinus fontinalis
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.14.99.1
(4Z,7Z,10Z,13Z,16Z,19Z)-docosahexaenoic acid + AH2 + O2
-
673508
Ovis aries
? + A + H2O
-
-
-
-
1.14.99.1
(4Z,7Z,10Z,13Z,16Z,19Z)-docosahexaenoic acid + AH2 + O2
-
673508
Salvelinus fontinalis
? + A + H2O
-
-
-
-
1.14.99.1
arachidonate + AH2 + O2
-
673508
Ovis aries
prostaglandin H2 + A + H2O
-
-
-
?
1.14.99.1
arachidonate + AH2 + O2
-
673508
Homo sapiens
prostaglandin H2 + A + H2O
-
-
-
?
1.14.99.1
arachidonate + AH2 + O2
-
673508
Salvelinus fontinalis
prostaglandin H2 + A + H2O
-
-
-
?
1.14.99.1
cis-11,14-eicosadienoic acid + AH2 + O2
-
673508
Homo sapiens
?
-
-
-
?
1.14.99.1
cis-4,7,10,13,16,19-docosahexaenoic acid + AH2 + O2
-
673508
Homo sapiens
?
-
-
-
?
1.14.99.1
cis-5,8,11,14,17-eicosapentaenoic acid + AH2 + O2
-
673508
Homo sapiens
?
-
-
-
?
1.14.99.1
cis-5,8,11,14-eicosatetraenoic acid + AH2 + O2
-
673508
Homo sapiens
?
-
-
-
?
1.14.99.1
cis-7,10,13,16-docosatetraenoic acid + AH2 + O2
-
673508
Homo sapiens
?
-
-
-
?
1.14.99.1
cis-8,11,14-eicosatrienoic acid + AH2 + O2
-
673508
Homo sapiens
?
-
-
-
?
1.14.99.1
gamma-linolenic acid + AH2 + O2
-
673508
Homo sapiens
?
-
-
-
?
1.14.99.1
linolenic acid + AH2 + O2
-
673508
Homo sapiens
?
-
-
-
?
Temperature Optimum [C] (protein specific)
EC Number
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
1.14.99.1
23
-
room temperature, 22-23C, lipoxygenase activity assay
Homo sapiens
1.14.99.1
23
-
room temperature, 22-23C, lipoxygenase activity assay
Salvelinus fontinalis
1.14.99.1
25
-
cyclooxygenase activity assay using a standard electrode
Homo sapiens
1.14.99.1
25
-
cyclooxygenase activity assay using a standard electrode
Ovis aries
1.14.99.1
25
-
cyclooxygenase activity assay using a standard electrode
Salvelinus fontinalis
1.14.99.1
30
-
cyclooxygenase activity assay using a high sensitivity electrode
Homo sapiens
1.14.99.1
30
-
cyclooxygenase activity assay using a high sensitivity electrode
Ovis aries
1.14.99.1
30
-
cyclooxygenase activity assay using a high sensitivity electrode
Salvelinus fontinalis
Temperature Range [C] (protein specific)
EC Number
Temperature Minimum [C]
Temperature Maximum [C]
Commentary
Organism
1.14.99.1
5
30
-
Homo sapiens
1.14.99.1
5
30
-
Ovis aries
1.14.99.1
5
30
-
Salvelinus fontinalis
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.14.99.1
7.2
-
cyclooxygenase activity assay
Homo sapiens
1.14.99.1
7.2
-
cyclooxygenase activity assay
Ovis aries
1.14.99.1
7.2
-
cyclooxygenase activity assay
Salvelinus fontinalis
1.14.99.1
8
-
lipoxygenase activity assay
Homo sapiens
1.14.99.1
8
-
lipoxygenase activity assay
Salvelinus fontinalis