Any feedback?
Literature summary extracted from
- NMR studies on the 46-kDa dimeric protein, 3,4-dihydroxy-2-butanone 4-phosphate synthase, using 2H, 13C, and 15N-labelling (1999), Eur. J. Biochem., 261, 57-65.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 4.1.99.12 | gene ribB, DNA determination and analysis, overexpression in strain M15 | Escherichia coli |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 4.1.99.12 | Mg2+ | required | Escherichia coli |  |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 4.1.99.12 | 23000 | - |
2 * 23000, SDS-PAGE, hydrodynamic analysis, NMR structure study, 2 * 23351, mass spectrometry | Escherichia coli |
| 4.1.99.12 | 23351 | - |
2 * 23000, SDS-PAGE, hydrodynamic analysis, NMR structure study, 2 * 23351, mass spectrometry | Escherichia coli |
| 4.1.99.12 | 44800 | - |
recombinant enzyme, analytical ultracentrifugation at 37°C | Escherichia coli |
| 4.1.99.12 | 46300 | - |
recombinant enzyme, hydrodynamic analysis, NMR structure study, analytical ultracentrifugation at 4°C, overview | Escherichia coli |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 4.1.99.12 | D-ribulose 5-phosphate | Escherichia coli | the enzyme is involved in the pathway of riboflavin biosynthesis | formate + L-3,4-dihydroxy-2-butanone-4-phosphate | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 4.1.99.12 | Escherichia coli | - |
gene ribB | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 4.1.99.12 | recombinant enzyme from strain M15 by anion exchange chromatography and gel filtration | Escherichia coli |
Reaction
| EC Number | Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|---|
| 4.1.99.12 | D-ribulose 5-phosphate = formate + L-3,4-dihydroxybutan-2-one 4-phosphate | catalytic mechanism | Escherichia coli |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 4.1.99.12 | 0.152 | - |
purified recombinant enzyme | Escherichia coli |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 4.1.99.12 | D-ribulose 5-phosphate | - |
Escherichia coli | formate + L-3,4-dihydroxy-2-butanone-4-phosphate | - |
? | |
| 4.1.99.12 | D-ribulose 5-phosphate | the enzyme is involved in the pathway of riboflavin biosynthesis | Escherichia coli | formate + L-3,4-dihydroxy-2-butanone-4-phosphate | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 4.1.99.12 | dimer | 2 * 23000, SDS-PAGE, hydrodynamic analysis, NMR structure study, 2 * 23351, mass spectrometry | Escherichia coli |
| 4.1.99.12 | More | hydrodynamic analysis, NMR structure study, isotope labeling, the homodimeric protein obeys strict C2 symmetry, overview | Escherichia coli |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 4.1.99.12 | 3,4-dihydroxy-2-butanone 4-phosphate synthase | - |
Escherichia coli |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
