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Literature summary extracted from

  • Yeom, S.; Kim, H.; Oh, D.
    Enantioselective production of 2,2-dimethylcyclopropane carboxylic acid from 2,2-dimethylcyclopropane carbonitrile using the nitrile hydratase and amidase of Rhodococcus erythropolis ATCC 25544 (2007), Enzyme Microb. Technol., 41, 842-848.
No PubMed abstract available

Application

EC Number Application Comment Organism
4.2.1.84 synthesis industrial production of (S)-2,2-dimethylcyclopropanecarboxylic acid Rhodococcus erythropolis

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.84 Comamonas oleophilus
-
 strain ATCC 15963
-
4.2.1.84 Nocardia transvalensis
-
 strain ATCC 12485
-
4.2.1.84 Rhodococcus erythropolis
-
 strain ATCC 25544
-
4.2.1.84 Rhodococcus rhodochrous
-
 strain ATCC 12674
-
4.2.1.84 Rhodococcus rhodochrous
-
 strain ATCC 33278
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.2.1.84 additional information
-
 0.078+/-0.003 micromol/h/mg cells, conversion of 2,2-dimethylcyclopropanecarbonitrile into 2,2-dimethylcyclopropanecarboxylic acid by nitrile hydratase/amidase Nocardia transvalensis
4.2.1.84 additional information
-
 0.096 +/- 0.003 micromol/h/mg cells, conversion of 2,2-dimethylcyclopropane carbonitrile into 2,2-dimethylcyclopropanecarboxylic acid by nitrile hydratase/amidase Rhodococcus erythropolis
4.2.1.84 additional information
-
 0.109 +/- 0.002 micromol/h/mg cells, conversion of 2,2-dimethylcyclopropanecarbonitrile into 2,2-dimethylcyclopropanecarboxylic acid by nitrile hydratase/amidase Rhodococcus rhodochrous
4.2.1.84 additional information
-
 0.220 +/- 0.006 micromol/h/mg cells, conversion of 2,2-dimethylcyclopropanecarbonitrile into 2,2-dimethylcyclopropanecarboxylic acid by nitrile hydratase/amidase Comamonas oleophilus
4.2.1.84 additional information
-
 0.438 +/- 0.01 micromol/h/mg cells, conversion of 2,2-dimethylcyclopropanecarbonitrile into 2,2-dimethylcyclopropanecarboxylic acid by nitrile hydratase/amidase Rhodococcus rhodochrous
4.2.1.84 additional information
-
 optimization of reaction conditions (pH, temperature, organic solvent) for conversion of 2,2-dimethylcyclopropanecarbonitrile into (S)-2,2-dimethylcyclopropanecarboxylic acid by nitrile hydratase/amidase enzyme system Rhodococcus erythropolis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.1.84 2,2-dimethylcyclopropanecarbonitrile + H2O
-
 Rhodococcus rhodochrous 2,2-dimethylcyclopropanecarboxamide
-
 ?
4.2.1.84 2,2-dimethylcyclopropanecarbonitrile + H2O
-
 Rhodococcus erythropolis 2,2-dimethylcyclopropanecarboxamide
-
 ?
4.2.1.84 2,2-dimethylcyclopropanecarbonitrile + H2O
-
 Nocardia transvalensis 2,2-dimethylcyclopropanecarboxamide
-
 ?
4.2.1.84 2,2-dimethylcyclopropanecarbonitrile + H2O
-
 Comamonas oleophilus 2,2-dimethylcyclopropanecarboxamide
-
 ?

Synonyms

EC Number Synonyms Comment Organism
4.2.1.84 nitrile hydratase
-
 Rhodococcus rhodochrous
4.2.1.84 nitrile hydratase
-
 Rhodococcus erythropolis
4.2.1.84 nitrile hydratase
-
 Nocardia transvalensis
4.2.1.84 nitrile hydratase
-
 Comamonas oleophilus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.2.1.84 30
-
 substrate: 2,2-dimethylcyclopropanecarbonitrile Rhodococcus erythropolis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2.1.84 7.5
-
 substrate: 2,2-dimethylcyclopropanecarbonitrile Rhodococcus erythropolis