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Literature summary extracted from

  • Chen, Y.; Huang, H.; Cheng, Y.; Tang, T.; Liu, W.
    Expression of a cholesterol oxidase gene from Arthrobacter simplex in Escherichia coli and Pichia pastoris (2006), Enzyme Microb. Technol., 39, 854-860.
No PubMed abstract available

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.3.6 gene choF, DNA and amino acid sequence determination and analysis, expression in Escherichia coli and Pichia pastoris Pimelobacter simplex

Organism

EC Number Organism UniProt Comment Textmining
1.1.3.6 Pimelobacter simplex Q56DL0 gene choF
-
1.1.3.6 Pimelobacter simplex F2 Q56DL0 gene choF
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.3.6 cholesterol + O2
-
Pimelobacter simplex cholest-4-en-3-one + H2O2
-
?
1.1.3.6 cholesterol + O2
-
Pimelobacter simplex F2 cholest-4-en-3-one + H2O2
-
?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.3.6 30
-
assay at Pimelobacter simplex

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.3.6 7.5
-
assay at Pimelobacter simplex