Any feedback?
Literature summary extracted from
- Expression of Penicillium variabile P16 glucose oxidase gene in Pichia pastoris and characterization of the recombinant enzyme (2006), Enzyme Microb. Technol., 39, 1230-1235.
No PubMed abstract available
Application
| EC Number | Application | Comment | Organism |
|---|---|---|---|
| 1.1.3.4 | analysis | the enzyme finds wide application in food industry and clinical analysis | Talaromyces variabilis |
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 1.1.3.4 | gene GOX, DNA sequence determination and analysis, expression in a larger scale in Pichia pastoris strain X33 using the methanol inducible AOX1 promoter, the enzyme is secreted | Talaromyces variabilis |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.1.3.4 | 13.5 | - |
D-glucose | pH 5.0, 28°C, native enzyme | Talaromyces variabilis |  |
| 1.1.3.4 | 15.25 | - |
D-glucose | pH 5.0, 28°C, recombinant enzyme | Talaromyces variabilis | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 1.1.3.4 | 82000 | - |
2 * 82000, recombinant enzyme, SDS-PAGE | Talaromyces variabilis |
| 1.1.3.4 | 175000 | - |
gel filtration | Talaromyces variabilis |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.1.3.4 | D-glucose + O2 | Talaromyces variabilis | - |
D-glucono-1,5-lactone + H2O2 | - |
? | |
| 1.1.3.4 | D-glucose + O2 | Talaromyces variabilis P16 | - |
D-glucono-1,5-lactone + H2O2 | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.1.3.4 | Talaromyces variabilis | Q70FC9 | - |
- |
| 1.1.3.4 | Talaromyces variabilis P16 | Q70FC9 | - |
- |
Posttranslational Modification
| EC Number | Posttranslational Modification | Comment | Organism |
|---|---|---|---|
| 1.1.3.4 | glycoprotein | the recombinant enzyme from Pichia pastoris is higher glycosylated, 17%, compared to the native enzyme, 14% | Talaromyces variabilis |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.1.3.4 | recombinant enzyme 2.4fold from Pichia pastoris culture supernatant by ultrafiltration, anion exchange chromatography, and gel filtration | Talaromyces variabilis |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 1.1.3.4 | 0.33 | - |
secreted enzyme in crude cell culture supernatant | Talaromyces variabilis |
| 1.1.3.4 | 144 | - |
purified recombinant enzyme | Talaromyces variabilis |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.1.3.4 | 2-deoxy-D-glucose + O2 | 19.6% of the activity with D-glucose for the native enzyme, 5.9 for the recombinant enzyme | Talaromyces variabilis | ? | - |
? | |
| 1.1.3.4 | D-fructose + O2 | 4.9% of the activity with D-glucose for the native enzyme, no activity with the recombinant enzyme | Talaromyces variabilis | ? | - |
? | |
| 1.1.3.4 | D-glucose + O2 | - |
Talaromyces variabilis | D-glucono-1,5-lactone + H2O2 | - |
? | |
| 1.1.3.4 | D-glucose + O2 | best substrate | Talaromyces variabilis | D-glucono-1,5-lactone + H2O2 | - |
? | |
| 1.1.3.4 | D-glucose + O2 | - |
Talaromyces variabilis P16 | D-glucono-1,5-lactone + H2O2 | - |
? | |
| 1.1.3.4 | D-glucose + O2 | best substrate | Talaromyces variabilis P16 | D-glucono-1,5-lactone + H2O2 | - |
? | |
| 1.1.3.4 | D-mannose + O2 | 7.2% of the activity with D-glucose for the native enzyme, 13.4 for the recombinant enzyme | Talaromyces variabilis | ? | - |
? | |
| 1.1.3.4 | D-mannose + O2 | 7.2% of the activity with D-glucose for the native enzyme, 13.4 for the recombinant enzyme | Talaromyces variabilis P16 | ? | - |
? | |
| 1.1.3.4 | D-xylose + O2 | 3.0% of the activity with D-glucose for the native enzyme, 5.8 for the recombinant enzyme | Talaromyces variabilis | ? | - |
? | |
| 1.1.3.4 | D-xylose + O2 | 3.0% of the activity with D-glucose for the native enzyme, 5.8 for the recombinant enzyme | Talaromyces variabilis P16 | ? | - |
? | |
| 1.1.3.4 | maltose + O2 | 21.3% of the activity with D-glucose for the native enzyme, 42.2% for the recombinant enzyme | Talaromyces variabilis | ? | - |
? | |
| 1.1.3.4 | additional information | no activity with L-arabinose and D-galactose with the native and recombinant enzyme | Talaromyces variabilis | ? | - |
? | |
| 1.1.3.4 | additional information | no activity with L-arabinose and D-galactose with the native and recombinant enzyme | Talaromyces variabilis P16 | ? | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 1.1.3.4 | dimer | 2 * 82000, recombinant enzyme, SDS-PAGE | Talaromyces variabilis |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 1.1.3.4 | GOX | - |
Talaromyces variabilis |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.1.3.4 | 45 | - |
recombinant enzyme | Talaromyces variabilis |
| 1.1.3.4 | 55 | - |
native enzyme | Talaromyces variabilis |
Temperature Range [°C]
| EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.1.3.4 | 30 | 60 | temperature profile of native enzyme, overview | Talaromyces variabilis |
| 1.1.3.4 | 30 | 55 | temperature profile of recombinant enzyme, overview | Talaromyces variabilis |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.1.3.4 | 40 | - |
half-life native enzyme: 103 min, half-life recombinant enzyme: 26 min | Talaromyces variabilis |
| 1.1.3.4 | 45 | - |
half-life native enzyme: 23 min, half-life recombinant enzyme: 3 min | Talaromyces variabilis |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.1.3.4 | additional information | - |
additional information | native enzyme and recombinant enzyme at pH 5.0, 28°C | Talaromyces variabilis |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.1.3.4 | 6 | - |
native and recombinant enzyme | Talaromyces variabilis |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.1.3.4 | 3 | 8 | pH-profiles, 54% of maximal activity at pH 3.0, 20% of maximal activity at pH 8.0 for the recombinant enzyme, 50% for the native enzyme, overview | Talaromyces variabilis |
pH Stability
| EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.1.3.4 | 3 | - |
half-life native enzyme: 166.3 min | Talaromyces variabilis |
| 1.1.3.4 | 7 | - |
half-life native enzyme: 76.3 min | Talaromyces variabilis |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.1.3.4 | FAD | bound | Talaromyces variabilis | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
