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Literature summary extracted from

  • Lee, Y.; Choi, J.J.; Kwon, S.
    Cloning, expression, and partial characterization of a family B-type DNA polymerase from the hyperthermophilic crenarchaeon Sulfophobococcus zilligii (2006), Enzyme Microb. Technol., 38, 821-830.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.7.7 bovine serum albumin DNA polymerase is stabilized with an approximately 25% increase in enzyme activity Sulfophobococcus zilligii
2.7.7.7 Triton X-100 DNA polymerase is stabilized with an approximately 25% increase in enzyme activity Sulfophobococcus zilligii

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.7.7 DNA polymerase gene is expressed under the control of the T7lac promoter on the expression vector pET-28a(+) in Escherichia coli BL21-CodonPlus(DE3)-RIL Sulfophobococcus zilligii

General Stability

EC Number General Stability Organism
2.7.7.7 DNA polymerase is stabilized by bovine serum albumin and Triton X-100 Sulfophobococcus zilligii

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.7.7 EDTA 2 mM, complete inhibition Sulfophobococcus zilligii
2.7.7.7 K+
-
Sulfophobococcus zilligii
2.7.7.7 NH4+
-
Sulfophobococcus zilligii

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.7.7 Mg2+ activates, optimal concentration is 1.5-2.5 mM Sulfophobococcus zilligii
2.7.7.7 Mn2+ optimal Mn2+ concentration is 0.5 mM Sulfophobococcus zilligii

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.7.7 89500
-
x * 89500, SDS-PAGE Sulfophobococcus zilligii
2.7.7.7 91268
-
x * 91268, calculated from sequence Sulfophobococcus zilligii

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.7 Sulfophobococcus zilligii Q29Y55
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.7.7 recombinant Sulfophobococcus zilligii

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.7.7 0.0033
-
-
Sulfophobococcus zilligii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.7 deoxynucleoside triphosphate + DNAn calf thymus DNA Sulfophobococcus zilligii diphosphate + DNAn+1
-
?
2.7.7.7 additional information Szi DNA polymerase possesses associated 3'-5' and 5'-3' exonuclease activities Sulfophobococcus zilligii ?
-
?

Subunits

EC Number Subunits Comment Organism
2.7.7.7 ? x * 89500, SDS-PAGE Sulfophobococcus zilligii
2.7.7.7 ? x * 91268, calculated from sequence Sulfophobococcus zilligii

Synonyms

EC Number Synonyms Comment Organism
2.7.7.7 family B-type DNA polymerase
-
Sulfophobococcus zilligii
2.7.7.7 Szi DNA polymerase
-
Sulfophobococcus zilligii

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.7.7 75
-
calf thymus DNA template Sulfophobococcus zilligii

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.7.7.7 60 90 60°C: about 45% of maximal activity, 90°C: about 40% of maximal activity, calf thymus DNA template Sulfophobococcus zilligii

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.7.7.7 95
-
half-life in presence of 0.02% bovine serum albumin: 4.5 h Sulfophobococcus zilligii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.7.7 6.5 7
-
Sulfophobococcus zilligii