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Literature summary extracted from

  • Watanabe, T.; Hattori, T.; Tengku, S.; Shimada, M.
    Purification and characterization of NAD-dependent formate dehydrogenase from the white-rot fungus Ceriporiopsis subvermispora and a possible role of the enzyme in oxalate metabolism (2005), Enzyme Microb. Technol., 37, 68-75.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.17.1.9 ADP-beta-D-ribose competitive with NAD+, mixed-type with formate Gelatoporia subvermispora
1.17.1.9 azide uncompetitive with NAD+, competitive with formate Gelatoporia subvermispora
1.17.1.9 bicarbonate mixed-type inhibition of NAD+ and formate Gelatoporia subvermispora
1.17.1.9 Hg2+ strong inhibition Gelatoporia subvermispora
1.17.1.9 iodoacetate strong inhibition Gelatoporia subvermispora
1.17.1.9 additional information not inhibitory: Mg2+, Mn2+, Co2+, Ni2+, Cd2+, Zn2+, Ca2+, Fe2+, Fe3+, Cu2+ Gelatoporia subvermispora
1.17.1.9 NADH competitive with NADH, mixed-type with formate Gelatoporia subvermispora
1.17.1.9 NADP+ strong inhibition Gelatoporia subvermispora
1.17.1.9 NADPH strong inhibition Gelatoporia subvermispora
1.17.1.9 p-chloromercuribenzoate strong inhibition Gelatoporia subvermispora

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.17.1.9 0.028
-
 NAD+ pH 6.5, 30°C Gelatoporia subvermispora
1.17.1.9 2.5
-
 formate pH 6.5, 30°C Gelatoporia subvermispora

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.17.1.9 42000
-
 2 * 42000, SDS-PAGE Gelatoporia subvermispora
1.17.1.9 84000
-
 gel filtration Gelatoporia subvermispora

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.17.1.9 additional information Gelatoporia subvermispora possible role of enzyme in oxalate metabolism ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.17.1.9 Gelatoporia subvermispora
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.17.1.9
-
 Gelatoporia subvermispora

Reaction

EC Number Reaction Comment Organism Reaction ID
1.17.1.9 formate + NAD+ = CO2 + NADH + H+ ordered BiBi mechanism with NAD+ and NADH being the first substrate and the second product, resp. Gelatoporia subvermispora
a

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.17.1.9 383.4
-
 pH 6.5, 30°C Gelatoporia subvermispora

Storage Stability

EC Number Storage Stability Organism
1.17.1.9 activity is retained in presence of dithiothreitol for 6 months, but lost within weeks in absence of SH compounds Gelatoporia subvermispora

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.17.1.9 formate + NAD+
-
 Gelatoporia subvermispora CO2 + NADH + H+
-
 ?
1.17.1.9 additional information possible role of enzyme in oxalate metabolism Gelatoporia subvermispora ?
-
 ?
1.17.1.9 additional information no substrate: acetate, pyruvate, malate, oxalate, succinate, methanol, isocitrate, fumarate Gelatoporia subvermispora ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.17.1.9 dimer 2 * 42000, SDS-PAGE Gelatoporia subvermispora

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.17.1.9 25 30
-
 Gelatoporia subvermispora

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.17.1.9 6.5
-
-
 Gelatoporia subvermispora

Cofactor

EC Number Cofactor Comment Organism Structure
1.17.1.9 NAD+ specific for NAD+ as electron acceptor Gelatoporia subvermispora

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.17.1.9 0.0000239
-
 azide substrate formate, pH 6.5, 30°C Gelatoporia subvermispora
1.17.1.9 0.00095
-
 azide substrate NAD+, pH 6.5, 30°C Gelatoporia subvermispora
1.17.1.9 0.0228
-
 ADP-beta-D-ribose substrate formate, pH 6.5, 30°C Gelatoporia subvermispora
1.17.1.9 0.0369
-
 ADP-beta-D-ribose substrate NAD+, pH 6.5, 30°C Gelatoporia subvermispora
1.17.1.9 0.053
-
 bicarbonate substrate formate, pH 6.5, 30°C Gelatoporia subvermispora
1.17.1.9 0.06
-
 NADH substrate NAD+, pH 6.5, 30°C Gelatoporia subvermispora
1.17.1.9 1.2
-
 bicarbonate substrate formate, pH 6.5, 30°C Gelatoporia subvermispora
1.17.1.9 5.6
-
 bicarbonate substrate NAD+, pH 6.5, 30°C Gelatoporia subvermispora