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Literature summary extracted from

  • Duvetter, T.; Van Loey, A.; Smout, C.; Verlent, I.; Nguyen, B.L.; Hendrickx, M.
    Aspergillus aculeatus pectin methylesterase: study of the inactivation by temperature and pressure and the inhibition by pectin methylesterase inhibitor (2005), Enzyme Microb. Technol., 36, 385-390.
No PubMed abstract available

Application

EC Number Application Comment Organism
3.1.1.11 food industry added as exogenous enzyme in fruit and vegetable processing, used to increase the yield during extraction, to clarify and concentrate fruit juices, for gelation of fruit, and to modify texture and rheology of fruit and vegetable based products Aspergillus aculeatus

General Stability

EC Number General Stability Organism
3.1.1.11 high pressure stabilizes enzyme activity Aspergillus aculeatus
3.1.1.11 highly pressure stable, no loss of activity upon treatment at 700 MPa for 1 h at 25°C Aspergillus aculeatus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.1.11 additional information no inhibition by proteinaceous pectin methylesterase inhibitor isolated from kiwi fruit Aspergillus aculeatus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.11 41000
-
 x * 41000, SDS-PAGE Aspergillus aculeatus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.1.11 pectin + H2O Aspergillus aculeatus involved in the regulation of the cell wall rigidity, key role in process of fruit ripening methanol + pectate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.11 Aspergillus aculeatus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.11
-
 Aspergillus aculeatus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.11 pectin + H2O
-
 Aspergillus aculeatus methanol + pectate
-
 ?
3.1.1.11 pectin + H2O involved in the regulation of the cell wall rigidity, key role in process of fruit ripening Aspergillus aculeatus methanol + pectate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.1.11 ? x * 41000, SDS-PAGE Aspergillus aculeatus

Synonyms

EC Number Synonyms Comment Organism
3.1.1.11 pectin methylesterase
-
 Aspergillus aculeatus
3.1.1.11 PME
-
 Aspergillus aculeatus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.1.11 55
-
 incubation at 55°C, atmospheric pressure an pH 4.5 for 10 min, more than 90% loss of activity, more than 75% of activity remaining after 30 min at 100 MPa Aspergillus aculeatus