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Literature summary extracted from

  • Papari-Zareei, M.; Brandmaier, A.; Auchus, R.J.
    Arginine 276 controls the directional preference of AKR1C9 (rat liver 3alpha-hydroxysteroid dehydrogenase) in human embryonic kidney 293 cells (2006), Endocrinology, 147, 1591-1597.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.50 stable expression of wild-type and mutant enzymes in HEK-293 cells Rattus norvegicus
1.1.1.213 stable expression of wild-type and mutant enzymes in HEK-293 cells Rattus norvegicus

Protein Variants

EC Number Protein Variants Comment Organism
1.1.1.50 R276E site-directed mutagenesis, the mutant shows increased preference for the oxidation reaction compared to the wild-type enzyme Rattus norvegicus
1.1.1.50 R276G site-directed mutagenesis, the mutant shows slightly increased preference for the reduction reaction compared to the wild-type enzyme Rattus norvegicus
1.1.1.50 R276M site-directed mutagenesis, the mutant shows slightly increased preference for the reduction reaction compared to the wild-type enzyme Rattus norvegicus
1.1.1.213 R276E site-directed mutagenesis, the mutant shows increased preference for the oxidation reaction compared to the wild-type enzyme Rattus norvegicus
1.1.1.213 R276G site-directed mutagenesis, the mutant shows slightly increased preference for the reduction reaction compared to the wild-type enzyme Rattus norvegicus
1.1.1.213 R276M site-directed mutagenesis, the mutant shows slightly increased preference for the reduction reaction compared to the wild-type enzyme Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.50 additional information
-
additional information equilibrium kinetics of wild-type and mutant enzymes Rattus norvegicus
1.1.1.213 additional information
-
additional information equilibrium kinetics of wild-type and mutant enzymes Rattus norvegicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.50 5alpha-dihydrotestosterone + NADPH Rattus norvegicus
-
5alpha-androstane-3alpha,17beta-diol + NADP+
-
?
1.1.1.213 5alpha-dihydrotestosterone + NADPH Rattus norvegicus
-
5alpha-androstane-3alpha,17beta-diol + NADP+
-
?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.50 Rattus norvegicus
-
-
-
1.1.1.213 Rattus norvegicus
-
-
-

Reaction

EC Number Reaction Comment Organism Reaction ID
1.1.1.50 a 3alpha-hydroxysteroid + NAD(P)+ = a 3-oxosteroid + NAD(P)H + H+ the reduction reaction is slightly preferred by the wild-type enzyme, which is determined by residue Arg276 Rattus norvegicus
1.1.1.213 a 3alpha-hydroxysteroid + NAD(P)+ = a 3-oxosteroid + NAD(P)H + H+ the reduction reaction is slightly preferred by the wild-type enzyme, which is determined by residue Arg276 Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.50 liver
-
Rattus norvegicus
-
1.1.1.213 liver
-
Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.50 additional information
-
-
Rattus norvegicus
1.1.1.213 additional information
-
-
Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.50 5alpha-dihydrotestosterone + NADPH
-
Rattus norvegicus 5alpha-androstane-3alpha,17beta-diol + NADP+
-
?
1.1.1.50 5alpha-dihydrotestosterone + NADPH i.e. DHT or 5alpha-androstane-17beta-ol-3-one, the reduction reaction is slightly preferred by the wild-type enzyme, which is determined by residue Arg276 Rattus norvegicus 5alpha-androstane-3alpha,17beta-diol + NADP+ i.e. 5alpha-androstane-3alpha,17beta-diol ?
1.1.1.213 5alpha-dihydrotestosterone + NADPH
-
Rattus norvegicus 5alpha-androstane-3alpha,17beta-diol + NADP+
-
?
1.1.1.213 5alpha-dihydrotestosterone + NADPH i.e. DHT or 5alpha-androstane-17beta-ol-3-one, the reduction reaction is slightly preferred by the wild-type enzyme, which is determined by residue Arg276 Rattus norvegicus 5alpha-androstane-3alpha,17beta-diol + NADP+ i.e. 5alpha-androstane-3alpha,17beta-diol ?

Synonyms

EC Number Synonyms Comment Organism
1.1.1.50 3alphaHSD
-
Rattus norvegicus
1.1.1.50 AKR1C9
-
Rattus norvegicus
1.1.1.50 More cf. EC 1.1.1.213 Rattus norvegicus
1.1.1.213 3alphaHSD
-
Rattus norvegicus
1.1.1.213 AKR1C9
-
Rattus norvegicus
1.1.1.213 More cf. EC 1.1.1.50 Rattus norvegicus

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.50 NAD(P)H 1000fold lower activity with NADH compared to NADPH Rattus norvegicus
1.1.1.213 NAD(P)H 1000fold lower activity with NADH compared to NADPH Rattus norvegicus