EC Number | Cloned (Comment) | Organism |
---|---|---|
1.1.1.50 | stable expression of wild-type and mutant enzymes in HEK-293 cells | Rattus norvegicus |
1.1.1.213 | stable expression of wild-type and mutant enzymes in HEK-293 cells | Rattus norvegicus |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
1.1.1.50 | R276E | site-directed mutagenesis, the mutant shows increased preference for the oxidation reaction compared to the wild-type enzyme | Rattus norvegicus |
1.1.1.50 | R276G | site-directed mutagenesis, the mutant shows slightly increased preference for the reduction reaction compared to the wild-type enzyme | Rattus norvegicus |
1.1.1.50 | R276M | site-directed mutagenesis, the mutant shows slightly increased preference for the reduction reaction compared to the wild-type enzyme | Rattus norvegicus |
1.1.1.213 | R276E | site-directed mutagenesis, the mutant shows increased preference for the oxidation reaction compared to the wild-type enzyme | Rattus norvegicus |
1.1.1.213 | R276G | site-directed mutagenesis, the mutant shows slightly increased preference for the reduction reaction compared to the wild-type enzyme | Rattus norvegicus |
1.1.1.213 | R276M | site-directed mutagenesis, the mutant shows slightly increased preference for the reduction reaction compared to the wild-type enzyme | Rattus norvegicus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.1.50 | additional information | - |
additional information | equilibrium kinetics of wild-type and mutant enzymes | Rattus norvegicus | |
1.1.1.213 | additional information | - |
additional information | equilibrium kinetics of wild-type and mutant enzymes | Rattus norvegicus |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.50 | 5alpha-dihydrotestosterone + NADPH | Rattus norvegicus | - |
5alpha-androstane-3alpha,17beta-diol + NADP+ | - |
? | |
1.1.1.213 | 5alpha-dihydrotestosterone + NADPH | Rattus norvegicus | - |
5alpha-androstane-3alpha,17beta-diol + NADP+ | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.1.1.50 | Rattus norvegicus | - |
- |
- |
1.1.1.213 | Rattus norvegicus | - |
- |
- |
EC Number | Reaction | Comment | Organism | Reaction ID |
---|---|---|---|---|
1.1.1.50 | a 3alpha-hydroxysteroid + NAD(P)+ = a 3-oxosteroid + NAD(P)H + H+ | the reduction reaction is slightly preferred by the wild-type enzyme, which is determined by residue Arg276 | Rattus norvegicus | |
1.1.1.213 | a 3alpha-hydroxysteroid + NAD(P)+ = a 3-oxosteroid + NAD(P)H + H+ | the reduction reaction is slightly preferred by the wild-type enzyme, which is determined by residue Arg276 | Rattus norvegicus |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
1.1.1.50 | liver | - |
Rattus norvegicus | - |
1.1.1.213 | liver | - |
Rattus norvegicus | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.1.1.50 | additional information | - |
- |
Rattus norvegicus |
1.1.1.213 | additional information | - |
- |
Rattus norvegicus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.50 | 5alpha-dihydrotestosterone + NADPH | - |
Rattus norvegicus | 5alpha-androstane-3alpha,17beta-diol + NADP+ | - |
? | |
1.1.1.50 | 5alpha-dihydrotestosterone + NADPH | i.e. DHT or 5alpha-androstane-17beta-ol-3-one, the reduction reaction is slightly preferred by the wild-type enzyme, which is determined by residue Arg276 | Rattus norvegicus | 5alpha-androstane-3alpha,17beta-diol + NADP+ | i.e. 5alpha-androstane-3alpha,17beta-diol | ? | |
1.1.1.213 | 5alpha-dihydrotestosterone + NADPH | - |
Rattus norvegicus | 5alpha-androstane-3alpha,17beta-diol + NADP+ | - |
? | |
1.1.1.213 | 5alpha-dihydrotestosterone + NADPH | i.e. DHT or 5alpha-androstane-17beta-ol-3-one, the reduction reaction is slightly preferred by the wild-type enzyme, which is determined by residue Arg276 | Rattus norvegicus | 5alpha-androstane-3alpha,17beta-diol + NADP+ | i.e. 5alpha-androstane-3alpha,17beta-diol | ? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.1.1.50 | 3alphaHSD | - |
Rattus norvegicus |
1.1.1.50 | AKR1C9 | - |
Rattus norvegicus |
1.1.1.50 | More | cf. EC 1.1.1.213 | Rattus norvegicus |
1.1.1.213 | 3alphaHSD | - |
Rattus norvegicus |
1.1.1.213 | AKR1C9 | - |
Rattus norvegicus |
1.1.1.213 | More | cf. EC 1.1.1.50 | Rattus norvegicus |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.50 | NAD(P)H | 1000fold lower activity with NADH compared to NADPH | Rattus norvegicus | |
1.1.1.213 | NAD(P)H | 1000fold lower activity with NADH compared to NADPH | Rattus norvegicus |