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Literature summary extracted from

  • Wohlgemuth, I.; Beringer, M.; Rodnina, M.V.
    Rapid peptide bond formation on isolated 50S ribosomal subunits (2006), EMBO Rep., 7, 699-703.
    View publication on PubMedView publication on EuropePMC

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.2.12 1.1
-
 puromycin at 37°C and pH 7.5 Escherichia coli
2.3.2.12 3.2
-
 cytidine-puromycin at 37°C and pH 7.5 Escherichia coli

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.3.2.12 Mg2+ the velocity of the reaction measured at limiting concentrations of Met-tRNA and saturating puromycin increases with Mg2+ concentration up to about 100 mM Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
2.3.2.12 Escherichia coli
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.2.12 Met-tRNA + cytidine-puromycin
-
 Escherichia coli tRNA + Met-cytidine-puromycin
-
 ?
2.3.2.12 Met-tRNA + puromycin
-
 Escherichia coli tRNA + Met-puromycin
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.3.2.12 peptidyl transferase
-
 Escherichia coli

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.3.2.12 0.17
-
 puromycin at 37°C and pH 7.5 Escherichia coli
2.3.2.12 19
-
 cytidine-puromycin at 37°C and pH 7.5 Escherichia coli