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Literature summary extracted from

  • Zhang, L.; Zhang, X.; Luo, Z.Y.
    Value of bilirubin oxidase and its mutants in the diagnosis of hyperbilirubinemia (2005), Di Yi Jun Yi Da Xue Xue Bao, 25, 1337-1340.
    View publication on PubMed

Application

EC Number Application Comment Organism
1.3.3.5 medicine mutants I402G and C457S are not appropriate for diagnostic purpose due to their much lower enzyme activities compared to the recombinant enzyme Aspergillus oryzae

Protein Variants

EC Number Protein Variants Comment Organism
1.3.3.5 C457S virtually no enzyme activity, Ru-incorporation conferrs higher enzyme activity Aspergillus oryzae
1.3.3.5 I402G low enzyme activity Aspergillus oryzae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.3.3.5 14.41
-
bilirubin mutant I402G Aspergillus oryzae
1.3.3.5 24.22
-
bilirubin recombinant enzyme Aspergillus oryzae

Organism

EC Number Organism UniProt Comment Textmining
1.3.3.5 Aspergillus oryzae
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.3.3.5 by gel filtration Aspergillus oryzae

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.3.3.5 additional information
-
mutant C457S shows no activity Aspergillus oryzae
1.3.3.5 1.67
-
mutant I402G Aspergillus oryzae
1.3.3.5 5.5
-
mutant 1Ru-C457S Aspergillus oryzae
1.3.3.5 6.42
-
mutant 2Ru-C457S Aspergillus oryzae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.3.5 bilirubin + O2
-
Aspergillus oryzae biliverdin + H2O
-
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Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.3.3.5 0.115
-
bilirubin mutant I402G Aspergillus oryzae
1.3.3.5 3.93
-
bilirubin recombinant enzyme Aspergillus oryzae

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.3.3.5 6 7.6
-
Aspergillus oryzae