EC Number | Application | Comment | Organism |
---|---|---|---|
1.3.3.5 | medicine | mutants I402G and C457S are not appropriate for diagnostic purpose due to their much lower enzyme activities compared to the recombinant enzyme | Aspergillus oryzae |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
1.3.3.5 | C457S | virtually no enzyme activity, Ru-incorporation conferrs higher enzyme activity | Aspergillus oryzae |
1.3.3.5 | I402G | low enzyme activity | Aspergillus oryzae |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.3.3.5 | 14.41 | - |
bilirubin | mutant I402G | Aspergillus oryzae | |
1.3.3.5 | 24.22 | - |
bilirubin | recombinant enzyme | Aspergillus oryzae |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.3.3.5 | Aspergillus oryzae | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.3.3.5 | by gel filtration | Aspergillus oryzae |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.3.3.5 | additional information | - |
mutant C457S shows no activity | Aspergillus oryzae |
1.3.3.5 | 1.67 | - |
mutant I402G | Aspergillus oryzae |
1.3.3.5 | 5.5 | - |
mutant 1Ru-C457S | Aspergillus oryzae |
1.3.3.5 | 6.42 | - |
mutant 2Ru-C457S | Aspergillus oryzae |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.3.3.5 | bilirubin + O2 | - |
Aspergillus oryzae | biliverdin + H2O | - |
? |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.3.3.5 | 0.115 | - |
bilirubin | mutant I402G | Aspergillus oryzae | |
1.3.3.5 | 3.93 | - |
bilirubin | recombinant enzyme | Aspergillus oryzae |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
1.3.3.5 | 6 | 7.6 | - |
Aspergillus oryzae |