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Literature summary extracted from

  • Janardhan, S.; Srivani, P.; Sastry, G.N.
    Choline kinase: an important target for cancer (2006), Curr. Med. Chem., 13, 1169-1186.
    View publication on PubMed

Application

EC Number Application Comment Organism
2.7.1.32 medicine target in anti-cancer therapy Homo sapiens

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.7.1.32
-
 Saccharomyces cerevisiae
2.7.1.32
-
 Schizosaccharomyces pombe
2.7.1.32
-
 Caenorhabditis elegans

Protein Variants

EC Number Protein Variants Comment Organism
2.7.1.32 D255A complete loss of activity Homo sapiens
2.7.1.32 E125A 2-25% catalytic efficiency compared to the wild type enzyme Homo sapiens
2.7.1.32 E125A modest change in kcat/Km with a marked decrease in the affinity for Ca2+ and a significant increase in Km for Mg2+ Homo sapiens
2.7.1.32 E255A almost complete loss of activity Homo sapiens
2.7.1.32 E303A 10fold decrease in activity Homo sapiens
2.7.1.32 E320A 26fold higher Km for ATP Homo sapiens
2.7.1.32 N255A complete loss of activity Homo sapiens
2.7.1.32 R111A 2-25% catalytic efficiency compared to the wild type enzyme Homo sapiens
2.7.1.32 S86A 2-25% catalytic efficiency compared to the wild type enzyme Homo sapiens
2.7.1.32 W387A 2-25% catalytic efficiency compared to the wild type enzyme Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.32 4'-bispyridyl-5,5'-perfluoroalkyl-2,2'-bisoxazol
-
 Homo sapiens
2.7.1.32 5'-dithio-bis-(2-nitrobenzoic acid) modest inhibitory activity Homo sapiens
2.7.1.32 choline
-
 Homo sapiens
2.7.1.32 cyclophane
-
 Homo sapiens
2.7.1.32 hemicholinium
-
 Homo sapiens
2.7.1.32 hemicholinium-3 weak inhibitor, IC50: 0.057 mM Homo sapiens
2.7.1.32 N-ethylmaleimide modest inhibitory activity Homo sapiens
2.7.1.32 N-[2-bromocinnamyl(amino)ethyl]-5-isoquinoline sulfonamide
-
 Homo sapiens
2.7.1.32 piperazine modest inhibitory activity Homo sapiens
2.7.1.32 purinyl-6-histamine modest inhibitory activity Homo sapiens
2.7.1.32 Quinacrine indirect inhibitor Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.1.32 cytosol
-
 Mus musculus 5829
-
2.7.1.32 cytosol
-
 Homo sapiens 5829
-
2.7.1.32 cytosol
-
 Rattus norvegicus 5829
-
2.7.1.32 cytosol
-
 Saccharomyces cerevisiae 5829
-
2.7.1.32 cytosol
-
 Schizosaccharomyces pombe 5829
-
2.7.1.32 cytosol
-
 Caenorhabditis elegans 5829
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.32 Ca2+ required Mus musculus
2.7.1.32 Ca2+ required Homo sapiens
2.7.1.32 Ca2+ required Rattus norvegicus
2.7.1.32 Ca2+ required Saccharomyces cerevisiae
2.7.1.32 Ca2+ required Schizosaccharomyces pombe
2.7.1.32 Ca2+ required Caenorhabditis elegans
2.7.1.32 Mg2+ required Mus musculus
2.7.1.32 Mg2+ required Homo sapiens
2.7.1.32 Mg2+ required Rattus norvegicus
2.7.1.32 Mg2+ required Saccharomyces cerevisiae
2.7.1.32 Mg2+ required Schizosaccharomyces pombe
2.7.1.32 Mg2+ required Caenorhabditis elegans

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.32 12750
-
 4 * 12750 Rattus norvegicus
2.7.1.32 13000
-
 4 * 13000 Mus musculus
2.7.1.32 42000
-
-
 Caenorhabditis elegans
2.7.1.32 51000
-
-
 Rattus norvegicus
2.7.1.32 52000
-
-
 Mus musculus
2.7.1.32 52000
-
 CHKA Homo sapiens
2.7.1.32 52000
-
 1 * 52000 Schizosaccharomyces pombe
2.7.1.32 66000
-
 1 * 66000 Saccharomyces cerevisiae

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.32 Caenorhabditis elegans
-
-
-
2.7.1.32 Homo sapiens
-
-
-
2.7.1.32 Mus musculus
-
-
-
2.7.1.32 Rattus norvegicus
-
-
-
2.7.1.32 Saccharomyces cerevisiae
-
-
-
2.7.1.32 Schizosaccharomyces pombe
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.1.32 brain
-
 Rattus norvegicus
-
2.7.1.32 breast
-
 Homo sapiens
-
2.7.1.32 colon
-
 Homo sapiens
-
2.7.1.32 kidney
-
 Rattus norvegicus
-
2.7.1.32 liver
-
 Rattus norvegicus
-
2.7.1.32 lung
-
 Homo sapiens
-
2.7.1.32 lung
-
 Rattus norvegicus
-
2.7.1.32 lymphoma cell
-
 Homo sapiens
-
2.7.1.32 neuroblastoma cell
-
 Homo sapiens
-
2.7.1.32 prostate
-
 Homo sapiens
-
2.7.1.32 testis
-
 Mus musculus
-
2.7.1.32 testis
-
 Homo sapiens
-
2.7.1.32 testis
-
 Rattus norvegicus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.32 ATP + choline
-
 Mus musculus ADP + O-phosphocholine
-
 ?
2.7.1.32 ATP + choline
-
 Homo sapiens ADP + O-phosphocholine
-
 ?
2.7.1.32 ATP + choline
-
 Rattus norvegicus ADP + O-phosphocholine
-
 ?
2.7.1.32 ATP + choline
-
 Saccharomyces cerevisiae ADP + O-phosphocholine
-
 ?
2.7.1.32 ATP + choline
-
 Schizosaccharomyces pombe ADP + O-phosphocholine
-
 ?
2.7.1.32 ATP + choline
-
 Caenorhabditis elegans ADP + O-phosphocholine
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.1.32 homotetramer
-
 Homo sapiens
2.7.1.32 homotetramer 4 * 12750 Rattus norvegicus
2.7.1.32 homotetramer 4 * 13000 Mus musculus
2.7.1.32 monomer 1 * 66000 Saccharomyces cerevisiae
2.7.1.32 monomer 1 * 52000 Schizosaccharomyces pombe

Synonyms

EC Number Synonyms Comment Organism
2.7.1.32 Chka
-
 Mus musculus
2.7.1.32 Chka
-
 Homo sapiens
2.7.1.32 Chka
-
 Rattus norvegicus
2.7.1.32 ChoK
-
 Mus musculus
2.7.1.32 ChoK
-
 Homo sapiens
2.7.1.32 ChoK
-
 Rattus norvegicus
2.7.1.32 choline kinase alpa
-
 Homo sapiens
2.7.1.32 choline kinase alpha
-
 Mus musculus
2.7.1.32 choline kinase alpha
-
 Rattus norvegicus
2.7.1.32 ckb
-
 Caenorhabditis elegans
2.7.1.32 CKII
-
 Saccharomyces cerevisiae
2.7.1.32 putative choline kinase
-
 Schizosaccharomyces pombe

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.32 8 9.5
-
 Homo sapiens

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.1.32 ATP
-
 Mus musculus
2.7.1.32 ATP
-
 Homo sapiens
2.7.1.32 ATP
-
 Rattus norvegicus
2.7.1.32 ATP
-
 Saccharomyces cerevisiae
2.7.1.32 ATP
-
 Schizosaccharomyces pombe
2.7.1.32 ATP
-
 Caenorhabditis elegans

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
2.7.1.32 0.057
-
 weak inhibitor, IC50: 0.057 mM Homo sapiens hemicholinium-3