Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • King, R.S.; Ghosh, A.A.; Wu, J.
    Inhibition of human phenol and estrogen sulfotransferase by certain non-steroidal anti-inflammatory agents (2006), Curr. Drug Metab., 7, 745-753.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.8.2.1 overexpression of SULT1A1 in Escherichia coli strain XL-1 Blue cytosol Homo sapiens
2.8.2.4 functional overexpression of SULT1E1 in Escherichia coli strain XL-1 blue cytosol Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.8.2.1 Acetylsalicylate binds near enough to the substrate site to prevent catalysis but does not affect dissociation of the substrate-enzyme complex Homo sapiens
2.8.2.1 Ibuprofen
-
 Homo sapiens
2.8.2.1 indomethacin
-
 Homo sapiens
2.8.2.1 meclofenamate binds near enough to the substrate site to prevent catalysis but does not affect dissociation of the substrate-enzyme complex Homo sapiens
2.8.2.1 additional information oxidation of phenolic substrates to carboxylates inhibits the enzyme in a competitive, reversible manner Homo sapiens
2.8.2.1 naproxen
-
 Homo sapiens
2.8.2.1 nimesulide binds near enough to the substrate site to prevent catalysis but not affect dissociation of the substrate-enzyme complex Homo sapiens
2.8.2.1 piroxicam
-
 Homo sapiens
2.8.2.1 salicylate
-
 Homo sapiens
2.8.2.1 sulindac
-
 Homo sapiens
2.8.2.4 Acetylsalicylate binds near enough to the substrate site to prevent catalysis but not affect dissociation of the substrate-enzyme complex Homo sapiens
2.8.2.4 Ibuprofen
-
 Homo sapiens
2.8.2.4 indomethacin
-
 Homo sapiens
2.8.2.4 meclofenamate binds near enough to the substrate site to prevent catalysis but not affect dissociation of the substrate-enzyme complex Homo sapiens
2.8.2.4 naproxen
-
 Homo sapiens
2.8.2.4 nimesulide binds near enough to the substrate site to prevent catalysis but not affect dissociation of the substrate-enzyme complex Homo sapiens
2.8.2.4 piroxicam
-
 Homo sapiens
2.8.2.4 salicylate
-
 Homo sapiens
2.8.2.4 sulindac
-
 Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.8.2.1 additional information
-
 additional information kinetic constants of competitive inhibitors Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.8.2.1 cytosol
-
 Homo sapiens 5829
-
2.8.2.4 cytosol
-
 Homo sapiens 5829
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.8.2.4 Mg2+
-
 Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.8.2.1 3'-phosphoadenylyl sulfate + 4-nitrophenol Homo sapiens
-
 adenosine 3',5'-bisphosphate + 4-nitrophenyl sulfate
-
 ?
2.8.2.4 3'-phosphoadenylyl sulfate + beta-estradiol Homo sapiens
-
 adenosine 3',5'-bisphosphate + beta-estradiol 3-sulfate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.8.2.1 Homo sapiens P50225
-
-
2.8.2.4 Homo sapiens P49888
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.8.2.1 liver
-
 Homo sapiens
-
2.8.2.4 liver
-
 Homo sapiens
-

Storage Stability

EC Number Storage Stability Organism
2.8.2.1 -70°C, liver cytosol, 50 mM Tris-HCl, pH 7.8, 0.25 M sucrose, 0.5 mM EDTA, 0.1 mM DTT, and 0.02 mM BHT, no loss of activity during storage Homo sapiens
2.8.2.4 -70°C, liver cytosol, 50 mM Tris-HCl, pH 7.8, 0.25 M sucrose, 0.5 mM EDTA, 0.1 mM DTT, and 0.02 mM BHT, no loss of activity during storage Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.8.2.1 3'-phosphoadenylyl sulfate + 4-nitrophenol
-
 Homo sapiens adenosine 3',5'-bisphosphate + 4-nitrophenyl sulfate
-
 ?
2.8.2.4 3'-phosphoadenylyl sulfate + beta-estradiol
-
 Homo sapiens adenosine 3',5'-bisphosphate + beta-estradiol 3-sulfate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.8.2.1 phenol sulfotransferase
-
 Homo sapiens
2.8.2.1 SULT1A1
-
 Homo sapiens
2.8.2.4 estrogen sulfotransferase
-
 Homo sapiens
2.8.2.4 SULT1E1
-
 Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.8.2.1 37
-
 assay at Homo sapiens
2.8.2.4 37
-
 assay at Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.8.2.1 7.4
-
 assay at Homo sapiens
2.8.2.4 6.5
-
 assay at Homo sapiens

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.8.2.1 0.000045
-
 meclofenamate pH 7.4, 37°C, versus 4-nitrophenol Homo sapiens
2.8.2.1 0.000056
-
 meclofenamate pH 7.4, 37°C, versus 3'-phosphoadenylyl sulfate Homo sapiens
2.8.2.1 0.00071
-
 nimesulide pH 7.4, 37°C, versus 4-nitrophenol Homo sapiens
2.8.2.1 0.00099
-
 nimesulide pH 7.4, 37°C, versus 3'-phosphoadenylyl sulfate Homo sapiens
2.8.2.1 0.0014
-
 Acetylsalicylate pH 7.4, 37°C, versus 4-nitrophenol Homo sapiens
2.8.2.1 0.0024
-
 Acetylsalicylate pH 7.4, 37°C, versus 3'-phosphoadenylyl sulfate Homo sapiens

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
2.8.2.1 0.00011
-
 pH 7.4, 37°C, versus 4-nitrophenol Homo sapiens meclofenamate
2.8.2.1 0.0012
-
 pH 7.4, 37°C, versus 4-nitrophenol Homo sapiens nimesulide
2.8.2.1 0.15
-
 pH 7.4, 37°C, versus 4-nitrophenol Homo sapiens salicylic acid
2.8.2.1 0.38
-
 pH 7.4, 37°C, versus 4-nitrophenol Homo sapiens piroxicam
2.8.2.1 0.39
-
 pH 7.4, 37°C, versus 4-nitrophenol Homo sapiens piroxicam
2.8.2.1 1.3
-
 pH 7.4, 37°C, versus 4-nitrophenol Homo sapiens Acetylsalicylate
2.8.2.1 1.7
-
 pH 7.4, 37°C, versus 4-nitrophenol Homo sapiens naproxen
2.8.2.1 1.9
-
 pH 7.4, 37°C, versus 4-nitrophenol Homo sapiens Ibuprofen
2.8.2.1 3.8
-
 pH 7.4, 37°C, versus 4-nitrophenol Homo sapiens sulindac
2.8.2.4 0.0065
-
 pH 6.5, 37°C, versus beta-estradiol Homo sapiens meclofenamate
2.8.2.4 0.038
-
 pH 6.5, 37°C, versus beta-estradiol Homo sapiens sulindac
2.8.2.4 0.14
-
 pH 6.5, 37°C, versus beta-estradiol Homo sapiens Ibuprofen
2.8.2.4 0.19
-
 pH 6.5, 37°C, versus beta-estradiol Homo sapiens naproxen
2.8.2.4 0.23
-
 pH 6.5, 37°C, versus beta-estradiol Homo sapiens nimesulide
2.8.2.4 0.27
-
 pH 6.5, 37°C, versus beta-estradiol Homo sapiens piroxicam
2.8.2.4 0.91
-
 pH 6.5, 37°C, versus beta-estradiol Homo sapiens salicylic acid
2.8.2.4 1.5
-
 pH 6.5, 37°C, versus beta-estradiol Homo sapiens Acetylsalicylate
2.8.2.4 9.4
-
 pH 6.5, 37°C, versus beta-estradiol Homo sapiens piroxicam