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Literature summary extracted from

  • Abdalla, A.; El-Mogy, M.; Farid, N.M.; El-Sharabasy, M.
    Two glutathione S-transferase isoenzymes purified from Bulinus truncatus (Gastropoda: Planorbidae) (2006), Comp. Biochem. Physiol. B, 143B, 76-84.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.5.1.18 bromosulfophthalein
-
 Bulinus truncatus
2.5.1.18 Cibacron blue
-
 Bulinus truncatus
2.5.1.18 Ethacrynic acid
-
 Bulinus truncatus
2.5.1.18 Hematin
-
 Bulinus truncatus
2.5.1.18 Indocyanine green
-
 Bulinus truncatus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5.1.18 0.0054
-
 glutathione pH 8.5, 25°C, isozyme GST2 Bulinus truncatus
2.5.1.18 0.0091
-
 glutathione pH 6.0, 25°C, isozyme GST2 Bulinus truncatus
2.5.1.18 0.011
-
 1-chloro-2,4-dinitrobenzene pH 8.5, 25°C, isozyme GST3 Bulinus truncatus
2.5.1.18 0.012
-
 glutathione pH 7.0, 25°C, isozyme GST2 Bulinus truncatus
2.5.1.18 0.014
-
 1-chloro-2,4-dinitrobenzene pH 8.5, 25°C, isozyme GST2 Bulinus truncatus
2.5.1.18 0.0175
-
 glutathione pH 6.5, 25°C, isozyme GST2 Bulinus truncatus
2.5.1.18 0.024
-
 1-chloro-2,4-dinitrobenzene pH 7.0, 25°C, isozyme GST2 Bulinus truncatus
2.5.1.18 0.026
-
 1-chloro-2,4-dinitrobenzene pH 7.0, 25°C, isozyme GST3 Bulinus truncatus
2.5.1.18 0.037
-
 1-chloro-2,4-dinitrobenzene pH 6.5, 25°C, isozyme GST2 Bulinus truncatus
2.5.1.18 0.058
-
 1-chloro-2,4-dinitrobenzene pH 6.5, 25°C, isozyme GST3 Bulinus truncatus
2.5.1.18 0.061
-
 1-chloro-2,4-dinitrobenzene pH 6.0, 25°C, isozyme GST3 Bulinus truncatus
2.5.1.18 0.064
-
 glutathione pH 6.0, 25°C, isozyme GST3 Bulinus truncatus
2.5.1.18 0.11
-
 1-chloro-2,4-dinitrobenzene pH 6.0, 25°C, isozyme GST2 Bulinus truncatus
2.5.1.18 0.15
-
 glutathione pH 6.5, 25°C, isozyme GST3 Bulinus truncatus

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.18 Bulinus truncatus
-
 isozymes GST2 and GST3
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.18 native isozymes GST2 and GST3 by ion exchange and glutathione affinity chromatography to homogeneity Bulinus truncatus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.18 glutathione + 1-chloro-2,4-dinitrobenzene
-
 Bulinus truncatus chloride + 2,4-dinitrophenyl-glutathione
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.5.1.18 glutathione S-transferase
-
 Bulinus truncatus
2.5.1.18 GST
-
 Bulinus truncatus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.5.1.18 45
-
 isozyme GST2 Bulinus truncatus
2.5.1.18 50
-
 isozyme GST3 Bulinus truncatus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.5.1.18 25 70
-
 Bulinus truncatus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.5.1.18 additional information
-
-
 Bulinus truncatus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.5.1.18 6 6.5
-
 Bulinus truncatus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.5.1.18 6 8.5
-
 Bulinus truncatus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
2.5.1.18 3.5
-
 isozymes GST2 and GST3, inactivation Bulinus truncatus
2.5.1.18 6 9 20 h, isozymes GST2 and GST3, completely stable Bulinus truncatus

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
2.5.1.18 0.00003
-
 isozyme GST2 Bulinus truncatus Hematin
2.5.1.18 0.00044
-
 isozyme GST3 Bulinus truncatus Cibacron blue
2.5.1.18 0.00074
-
 isozyme GST3 Bulinus truncatus Ethacrynic acid
2.5.1.18 0.00076
-
 isozyme GST2 Bulinus truncatus Cibacron blue
2.5.1.18 0.00079
-
 isozyme GST2 Bulinus truncatus Ethacrynic acid
2.5.1.18 0.0076
-
 isozyme GST2 Bulinus truncatus Indocyanine green
2.5.1.18 0.0407
-
 isozyme GST3 Bulinus truncatus Hematin
2.5.1.18 0.0479
-
 isozyme GST2 Bulinus truncatus bromosulfophthalein
2.5.1.18 0.055
-
 isozyme GST3 Bulinus truncatus bromosulfophthalein
2.5.1.18 0.089
-
 isozyme GST3 Bulinus truncatus Indocyanine green