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Literature summary extracted from

  • Qi, X.; Sun, L.; Luo, Z.; Wu, J.; Meng, X.; Tang, Y.; Wei, Y.; Huang, R.
    Rational design of glycerol dehydratase: Swapping the genes encoding the subunits of glycerol dehydratase to improve enzymatic properties (2006), Chin. Sci. Bull., 51, 2977-2985.
No PubMed abstract available

Application

EC Number Application Comment Organism
4.2.1.30 synthesis synthesis of 1,3-propanediol Citrobacter freundii
4.2.1.30 synthesis synthesis of 1,3-propanediol Klebsiella pneumoniae

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.2.1.30 expression in Escherichia coli Citrobacter freundii
4.2.1.30 expression in Escherichia coli Klebsiella pneumoniae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.2.1.30 0.24
-
 1,2-propanediol
-
 Klebsiella pneumoniae
4.2.1.30 0.25
-
 1,2-propanediol
-
 Citrobacter freundii
4.2.1.30 0.59
-
 glycerol
-
 Citrobacter freundii
4.2.1.30 0.6
-
 glycerol
-
 Klebsiella pneumoniae

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.30 Citrobacter freundii A8CH84
-
-
4.2.1.30 Klebsiella pneumoniae A8CIU9
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.1.30 Ni-nitrilotriacetate affinity chromatography followed by Sephacral S-300 gel filtration Citrobacter freundii
4.2.1.30 Ni-nitrilotriacetate affinity chromatography followed by Sephacral S-300 gel filtration Klebsiella pneumoniae

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.2.1.30 91.9
-
-
 Citrobacter freundii
4.2.1.30 91.9
-
-
 Klebsiella pneumoniae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.1.30 1,2-propandiol
-
 Klebsiella pneumoniae propionaldehyde + H2O
-
 ?
4.2.1.30 1,2-propanediol
-
 Citrobacter freundii propionaldehyde + H2O
-
 ?
4.2.1.30 glycerol
-
 Citrobacter freundii 3-hydroxypropionaldehyde + H2O
-
 ?
4.2.1.30 glycerol
-
 Klebsiella pneumoniae 3-hydroxypropionaldehyde + H2O
-
 ?

Subunits

EC Number Subunits Comment Organism
4.2.1.30 hexamer heterohexamer of alpha2beta2gamma2 Citrobacter freundii
4.2.1.30 hexamer heterohexamer of alpha2beta2gamma2 Klebsiella pneumoniae

Synonyms

EC Number Synonyms Comment Organism
4.2.1.30 glycerol dehydratase
-
 Citrobacter freundii
4.2.1.30 glycerol dehydratase
-
 Klebsiella pneumoniae

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.2.1.30 45
-
-
 Citrobacter freundii
4.2.1.30 45
-
-
 Klebsiella pneumoniae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2.1.30 8.5
-
-
 Citrobacter freundii
4.2.1.30 8.5
-
-
 Klebsiella pneumoniae