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Literature summary extracted from

  • Milanese, L.; Espinosa, A.; Campos, J.M.; Gallo, M.A.; Entrena, A.
    Insight into the inhibition of human choline kinase: homology modeling and molecular dynamics simulations (2006), ChemMedChem, 1, 1216-1228.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
2.7.1.32 D255A omplete loss of activity Caenorhabditis elegans
2.7.1.32 D255E small residual activity Caenorhabditis elegans
2.7.1.32 D255N small residual activity Caenorhabditis elegans
2.7.1.32 D301A complete loss of activity Caenorhabditis elegans
2.7.1.32 D301E complete loss of activity Caenorhabditis elegans
2.7.1.32 D301N complete loss of activity Caenorhabditis elegans
2.7.1.32 E125A modest reduction of kcat and Km for ATP Caenorhabditis elegans
2.7.1.32 E125D no influence Caenorhabditis elegans
2.7.1.32 E125Q modest reduction of kcat and Km for ATP Caenorhabditis elegans
2.7.1.32 E303A strong reduction of the catalytic efficiency Caenorhabditis elegans
2.7.1.32 E303D small reduction of kcat Caenorhabditis elegans
2.7.1.32 E303N small reduction of kcat Caenorhabditis elegans
2.7.1.32 E320A reduction of ATP affinity and time instability Caenorhabditis elegans
2.7.1.32 H253A similar initial activity like wild type enzyme, but time instability Caenorhabditis elegans
2.7.1.32 N254A similar initial activity like wild type enzyme, but time instability Caenorhabditis elegans
2.7.1.32 N260A small residual activity Caenorhabditis elegans
2.7.1.32 N308 A small reduction of kcat Caenorhabditis elegans
2.7.1.32 N308D modest reduction of kcat Caenorhabditis elegans
2.7.1.32 N308Q modest reduction of kcat Caenorhabditis elegans
2.7.1.32 N87A small reduction of the catalytic efficiency Caenorhabditis elegans
2.7.1.32 R111A 4-fold increase in Km for ATP Caenorhabditis elegans
2.7.1.32 R149A no influence Caenorhabditis elegans
2.7.1.32 S86A pronounced reduction of the catalytic efficiency Caenorhabditis elegans
2.7.1.32 S86T moderate reduction of the catalytic efficiency Caenorhabditis elegans
2.7.1.32 W387A strong reduction of kcat (30-fold) Caenorhabditis elegans

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.32 Ca2+
-
 Caenorhabditis elegans
2.7.1.32 Ca2+
-
 Homo sapiens
2.7.1.32 hemicholinium-3
-
 Caenorhabditis elegans
2.7.1.32 hemicholinium-3
-
 Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.32 Caenorhabditis elegans
-
-
-
2.7.1.32 Homo sapiens
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.32 ATP + choline
-
 Homo sapiens ADP + O-phosphocholine
-
 ?
2.7.1.32 ATP + choline
-
 Caenorhabditis elegans ADP + O-phosphocholine
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.7.1.32 CK-alpha
-
 Homo sapiens
2.7.1.32 CKA-2
-
 Caenorhabditis elegans

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.1.32 ATP
-
 Homo sapiens
2.7.1.32 ATP
-
 Caenorhabditis elegans