EC Number | Cloned (Comment) | Organism |
---|---|---|
1.1.2.7 | DNA and amino acid sequence determination and analysis | Hyphomicrobium denitrificans |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
1.1.2.7 | additional information | preparation of an inactive Ca2+-free MDH-containing Hyphomicrobium denitrificans strain, which contains a fully-oxidized pyrroloquinoline quinone cofactor, incubation of Ca2+-free MDH with Ca2+ ion leads to reconstituted, fully active enzyme containing fully-reduced, tightly bound PQQ, elucidation of the Ca2+ ion elimination mechanism, overview | Hyphomicrobium denitrificans |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
1.1.2.7 | Ca2+ | absolutely required, 1.43 Ca2+ per enzyme tetramer, tightly bound to the active site, preparation of Ca2+-free MDH, which contains a fully-oxidized pyrroloquinoline quinone cofactor, incubation of Ca2+-free MDH with Ca2+ ion leads to reconstituted, fully active enzyme containing fully-reduced, tightly bound PQQ, overview, Ca2+-free enzyme is inactive | Hyphomicrobium denitrificans |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.1.2.7 | 9000 | - |
2 * 65000, alpha-subunit, + 2 * 9000, beta-subunit, SDS-PAGE | Hyphomicrobium denitrificans |
1.1.2.7 | 65000 | - |
2 * 65000, alpha-subunit, + 2 * 9000, beta-subunit, SDS-PAGE | Hyphomicrobium denitrificans |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.1.2.7 | Hyphomicrobium denitrificans | - |
- |
- |
1.1.2.7 | Hyphomicrobium denitrificans A3151 | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.1.2.7 | Ca2+-containing and Ca2+-free enzymes from cell-free extracts by ion exchange and hydrophobic interaction chromatography | Hyphomicrobium denitrificans |
EC Number | Reaction | Comment | Organism | Reaction ID |
---|---|---|---|---|
1.1.2.7 | a primary alcohol + 2 ferricytochrome cL = an aldehyde + 2 ferrocytochrome cL + 2 H+ | the amino-acid residues related to the active site of MDH, E55, C103, C104, R109, T159, S174, E177, T243, W243, N261, D303, R331, N394, and W476, are completely conserved | Hyphomicrobium denitrificans |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.1.2.7 | additional information | - |
Ca2+-free enzyme is inactive | Hyphomicrobium denitrificans |
1.1.2.7 | 13 | - |
purified reconstituted Ca2+-enzyme | Hyphomicrobium denitrificans |
1.1.2.7 | 18 | - |
purified native Ca2+-enzyme | Hyphomicrobium denitrificans |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.1.2.7 | tetramer | 2 * 65000, alpha-subunit, + 2 * 9000, beta-subunit, SDS-PAGE | Hyphomicrobium denitrificans |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.1.2.7 | MDH | - |
Hyphomicrobium denitrificans |
1.1.2.7 | methanol dehydrogenase | - |
Hyphomicrobium denitrificans |
1.1.5.5 | MDH | - |
- |
1.1.5.5 | quinoprotein methanol dehydrogenase | - |
- |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.1.2.7 | pyrroloquinoline quinone | bound to the enzyme, preparation of Ca2+-free MDH, which contains a fully-oxidized pyrroloquinoline quinone cofactor, incubation of Ca2+-free MDH with Ca2+ ion leads to reconstituted, fully active enzyme containing fully-reduced, tightly bound PQQ, overview | Hyphomicrobium denitrificans |