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Literature summary extracted from

  • Nojiri, M.; Hira, D.; Yamaguchi, K.; Okajima, T.; Tanizawa, K.; Suzuki, S.
    Preparation and characterization of Ca2+-free methanol dehydrogenase from Hyphomicrobium denitrificans A3151 (2005), Chem. Lett., 34, 1036-1037.
No PubMed abstract available

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.2.7 DNA and amino acid sequence determination and analysis Hyphomicrobium denitrificans

Protein Variants

EC Number Protein Variants Comment Organism
1.1.2.7 additional information preparation of an inactive Ca2+-free MDH-containing Hyphomicrobium denitrificans strain, which contains a fully-oxidized pyrroloquinoline quinone cofactor, incubation of Ca2+-free MDH with Ca2+ ion leads to reconstituted, fully active enzyme containing fully-reduced, tightly bound PQQ, elucidation of the Ca2+ ion elimination mechanism, overview Hyphomicrobium denitrificans

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.2.7 Ca2+ absolutely required, 1.43 Ca2+ per enzyme tetramer, tightly bound to the active site, preparation of Ca2+-free MDH, which contains a fully-oxidized pyrroloquinoline quinone cofactor, incubation of Ca2+-free MDH with Ca2+ ion leads to reconstituted, fully active enzyme containing fully-reduced, tightly bound PQQ, overview, Ca2+-free enzyme is inactive Hyphomicrobium denitrificans

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.2.7 9000
-
2 * 65000, alpha-subunit, + 2 * 9000, beta-subunit, SDS-PAGE Hyphomicrobium denitrificans
1.1.2.7 65000
-
2 * 65000, alpha-subunit, + 2 * 9000, beta-subunit, SDS-PAGE Hyphomicrobium denitrificans

Organism

EC Number Organism UniProt Comment Textmining
1.1.2.7 Hyphomicrobium denitrificans
-
-
-
1.1.2.7 Hyphomicrobium denitrificans A3151
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.2.7 Ca2+-containing and Ca2+-free enzymes from cell-free extracts by ion exchange and hydrophobic interaction chromatography Hyphomicrobium denitrificans

Reaction

EC Number Reaction Comment Organism Reaction ID
1.1.2.7 a primary alcohol + 2 ferricytochrome cL = an aldehyde + 2 ferrocytochrome cL + 2 H+ the amino-acid residues related to the active site of MDH, E55, C103, C104, R109, T159, S174, E177, T243, W243, N261, D303, R331, N394, and W476, are completely conserved Hyphomicrobium denitrificans

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.2.7 additional information
-
Ca2+-free enzyme is inactive Hyphomicrobium denitrificans
1.1.2.7 13
-
purified reconstituted Ca2+-enzyme Hyphomicrobium denitrificans
1.1.2.7 18
-
purified native Ca2+-enzyme Hyphomicrobium denitrificans

Subunits

EC Number Subunits Comment Organism
1.1.2.7 tetramer 2 * 65000, alpha-subunit, + 2 * 9000, beta-subunit, SDS-PAGE Hyphomicrobium denitrificans

Synonyms

EC Number Synonyms Comment Organism
1.1.2.7 MDH
-
Hyphomicrobium denitrificans
1.1.2.7 methanol dehydrogenase
-
Hyphomicrobium denitrificans
1.1.5.5 MDH
-
-
1.1.5.5 quinoprotein methanol dehydrogenase
-
-

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.2.7 pyrroloquinoline quinone bound to the enzyme, preparation of Ca2+-free MDH, which contains a fully-oxidized pyrroloquinoline quinone cofactor, incubation of Ca2+-free MDH with Ca2+ ion leads to reconstituted, fully active enzyme containing fully-reduced, tightly bound PQQ, overview Hyphomicrobium denitrificans