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Literature summary extracted from

  • Minagawa, H.; Yoshida, Y.; Kenmochi, N.; Furuichi, M.; Shimada, J.; Kaneko, H.
    Improving the thermal stability of lactate oxidase by directed evolution (2007), Cell. Mol. Life Sci., 64, 77-81.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.3.15 expression in Escherichia coli Aerococcus viridans

Protein Variants

EC Number Protein Variants Comment Organism
1.1.3.15 E160G/V198I the half-life of the mutant lactate oxidase at 70°C is about 18times that of the wild-type enzyme. Km-value for (S)-lactate at 25°C is 46fold higher than wild-type value, Km-value for (S)-lactate at 25°C is 59fold higher than wild-type value Aerococcus viridans
1.1.3.15 E160G/V198I/G36S/T103S/A232S/F277Y the half-life of the mutant lactate oxidase at 70°C is about 2 times that of E160G/V198I mutant and about 36 times that of the wild-type enzyme. Km-value for (S)-lactate at 25°C is 48fold higher than wild-type value, Km-value for (S)-lactate at 25°C is 55fold higher than wild-type value. Mutant is obtained by direct evolution Aerococcus viridans

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.3.15 0.529
-
(S)-lactate 25°C, wild-type enzyme Aerococcus viridans
1.1.3.15 0.863
-
(S)-lactate 35°C, wild-type enzyme Aerococcus viridans
1.1.3.15 24.3
-
(S)-lactate 25°C, mutant enzyme E160G/V198I Aerococcus viridans
1.1.3.15 25.5
-
(S)-lactate 25°C, mutant enzyme E160G/V198I/G36S/T103S/A232S/F277Y Aerococcus viridans
1.1.3.15 47.6
-
(S)-lactate 35°C, mutant enzyme E160G/V198I/G36S/T103S/A232S/F277Y Aerococcus viridans
1.1.3.15 50.7
-
(S)-lactate 35°C, mutant enzyme E160G/V198I Aerococcus viridans

Organism

EC Number Organism UniProt Comment Textmining
1.1.3.15 Aerococcus viridans
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.3.15 (S)-lactate + O2
-
Aerococcus viridans acetate + CO2 + H2O
-
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Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.3.15 70
-
t1/2: 1.7 min for wild-type enzyme, 30.3 min for mutant enzyme E160G/V198I, 61.4 min for mutant enzyme E160G/V198I/G36S/T103S/A232S/F277Y Aerococcus viridans