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Literature summary extracted from

  • Park, J.; Kim, H.; Kim, Y.; Cha, H.; Kim, Y.; Kim, T.; Kim, Y.; Park, K.
    The action mode of Thermus aquaticus YT-1 4-?-glucanotransferase and its chimeric enzymes introduced with starch-binding domain on amylose and amylopectin (2007), Carbohydr. Polym., 67, 164-173.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.25 expression in Escherichia coli Thermus aquaticus

Protein Variants

EC Number Protein Variants Comment Organism
2.4.1.25 additional information the starch-binding domains of Bacillus stearothermophilus ET1 CGTase (E and DE) are introduced into the C-terminus of TAalphaGT to enhance the starch utilizing activity. The chimeric enzymes, TAalphaGT-E and TAalphaGT-DE, show no difference in temperature optimum, transglycosylation activity, and amylolytic degradation pattern compared to TAalphaGT wild-type. However, TAalphaGT-DE exhibits the highest molar specific activity toward amylose. TAalphaGT-DE modifies amylopectin molecules by its disproportionating activities to produce modified amylopectin clusters (MW 100000–1000000) and produces cyclo-amyloses with DP of 19 through 35 from amylose molecules Thermus aquaticus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.1.25 57969
-
 x * 57969, calculated from sequence Thermus aquaticus

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.25 Thermus aquaticus
-
-
-
2.4.1.25 Thermus aquaticus YT-1
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.25
-
 Thermus aquaticus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.25 amylose
-
 Thermus aquaticus ?
-
 ?
2.4.1.25 amylose
-
 Thermus aquaticus YT-1 ?
-
 ?
2.4.1.25 additional information TAalphaGT catalyzes the transfer of glucose units from one 1,4-alpha-glucan to another and requires at least maltose units for the disproportionation reaction Thermus aquaticus ?
-
 ?
2.4.1.25 additional information TAalphaGT catalyzes the transfer of glucose units from one 1,4-alpha-glucan to another and requires at least maltose units for the disproportionation reaction Thermus aquaticus YT-1 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.4.1.25 ? x * 57969, calculated from sequence Thermus aquaticus

Synonyms

EC Number Synonyms Comment Organism
2.4.1.25 TAalphaGT
-
 Thermus aquaticus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.25 70
-
 substrate: amylose Thermus aquaticus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.4.1.25 80
-
 120 min, 10% loss of activity Thermus aquaticus
2.4.1.25 85
-
 210 min, 10fold decrease in activity Thermus aquaticus
2.4.1.25 90
-
 29 min, 10fold decrease in activity Thermus aquaticus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.25 7.5
-
 substrate: amylose Thermus aquaticus