BRENDA - Enzyme Database show

Positive regulation of deoxycytidine kinase activity by phosphorylation of Ser-74 in B-cell chronic lymphocytic leukemia lymphocytes

Smal, C.; Van Den Neste, E.; Maerevoet, M.; Poire, X.; Theate, I.; Bontemps, F.; Cancer Lett. 253, 68-73 (2007)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
2.7.1.74
2-chloro-2'-deoxyadenosine
increases activity by increasing phosphorylation of Ser74
Homo sapiens
2.7.1.74
aphidicolin
increases activity by increasing phosphorylation of Ser74
Homo sapiens
2.7.1.74
etoposide
increases activity by increasing phosphorylation of Ser74
Homo sapiens
2.7.1.74
genistein
increases activity by increasing phosphorylation of Ser74
Homo sapiens
2.7.1.74
additional information
UV-C irradiation increases activity by increasing phosphorylation of Ser74
Homo sapiens
Application
EC Number
Application
Commentary
Organism
2.7.1.74
analysis
deoxycytidine kinase from B-cell chronic lymphocytic leukaemia lymphocytes can be detected by use of anti-phospho-ser74 antibodies
Homo sapiens
2.7.1.74
medicine
interindividual variability in deoxycytidine kinase activity is related to its phosphorylation level on residue Ser74
Homo sapiens
Cloned(Commentary)
EC Number
Commentary
Organism
2.7.1.74
expressed in HEK 293T cells
Homo sapiens
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
2.7.1.74
Mg2+
in complex with ATP
Homo sapiens
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.7.1.74
NTP + 2-chloro-2'-deoxyadenosine
Homo sapiens
phosphorylation of chemotherapeutically important nucleoside analogues
NDP + 2-chloro-2'-deoxyadenosine 5'-phosphate
-
-
?
2.7.1.74
NTP + 9-beta-D-arabinosyl-2-fluoroadenine
Homo sapiens
phosphorylation of chemotherapeutically important nucleoside analogues
NDP + 9-beta-D-arabinosyl-2-fluoroadenine 5'-phosphate
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.7.1.74
Homo sapiens
-
; patients with B-cell chronic lymphocytic leukaemia
-
Posttranslational Modification
EC Number
Posttranslational Modification
Commentary
Organism
2.7.1.74
phosphoprotein
interindividual variability in deoxycytidine kinase activity is related to its phosphorylation level on residue Ser74; regulation of activity by phosphorylation of Ser-74
Homo sapiens
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
2.7.1.74
B-CLL cell
-
Homo sapiens
-
2.7.1.74
blood
-
Homo sapiens
-
2.7.1.74
lymphocyte
B-cell chronic lymphocytic leukaemia lymphocyte
Homo sapiens
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.1.74
ATP + 2'-deoxycytidine
-
672894
Homo sapiens
ADP + 2'-deoxy-CMP
-
-
-
?
2.7.1.74
NTP + 2-chloro-2'-deoxyadenosine
phosphorylation of chemotherapeutically important nucleoside analogues
672894
Homo sapiens
NDP + 2-chloro-2'-deoxyadenosine 5'-phosphate
-
-
-
?
2.7.1.74
NTP + 9-beta-D-arabinosyl-2-fluoroadenine
phosphorylation of chemotherapeutically important nucleoside analogues
672894
Homo sapiens
NDP + 9-beta-D-arabinosyl-2-fluoroadenine 5'-phosphate
-
-
-
?
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
2.7.1.74
ATP
-
Homo sapiens
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
2.7.1.74
2-chloro-2'-deoxyadenosine
increases activity by increasing phosphorylation of Ser74
Homo sapiens
2.7.1.74
aphidicolin
increases activity by increasing phosphorylation of Ser74
Homo sapiens
2.7.1.74
etoposide
increases activity by increasing phosphorylation of Ser74
Homo sapiens
2.7.1.74
genistein
increases activity by increasing phosphorylation of Ser74
Homo sapiens
2.7.1.74
additional information
UV-C irradiation increases activity by increasing phosphorylation of Ser74
Homo sapiens
Application (protein specific)
EC Number
Application
Commentary
Organism
2.7.1.74
analysis
deoxycytidine kinase from B-cell chronic lymphocytic leukaemia lymphocytes can be detected by use of anti-phospho-ser74 antibodies
Homo sapiens
2.7.1.74
medicine
interindividual variability in deoxycytidine kinase activity is related to its phosphorylation level on residue Ser74
Homo sapiens
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
2.7.1.74
expressed in HEK 293T cells
Homo sapiens
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
2.7.1.74
ATP
-
Homo sapiens
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
2.7.1.74
Mg2+
in complex with ATP
Homo sapiens
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.7.1.74
NTP + 2-chloro-2'-deoxyadenosine
Homo sapiens
phosphorylation of chemotherapeutically important nucleoside analogues
NDP + 2-chloro-2'-deoxyadenosine 5'-phosphate
-
-
?
2.7.1.74
NTP + 9-beta-D-arabinosyl-2-fluoroadenine
Homo sapiens
phosphorylation of chemotherapeutically important nucleoside analogues
NDP + 9-beta-D-arabinosyl-2-fluoroadenine 5'-phosphate
-
-
?
Posttranslational Modification (protein specific)
EC Number
Posttranslational Modification
Commentary
Organism
2.7.1.74
phosphoprotein
interindividual variability in deoxycytidine kinase activity is related to its phosphorylation level on residue Ser74; regulation of activity by phosphorylation of Ser-74
Homo sapiens
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
2.7.1.74
B-CLL cell
-
Homo sapiens
-
2.7.1.74
blood
-
Homo sapiens
-
2.7.1.74
lymphocyte
B-cell chronic lymphocytic leukaemia lymphocyte
Homo sapiens
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.1.74
ATP + 2'-deoxycytidine
-
672894
Homo sapiens
ADP + 2'-deoxy-CMP
-
-
-
?
2.7.1.74
NTP + 2-chloro-2'-deoxyadenosine
phosphorylation of chemotherapeutically important nucleoside analogues
672894
Homo sapiens
NDP + 2-chloro-2'-deoxyadenosine 5'-phosphate
-
-
-
?
2.7.1.74
NTP + 9-beta-D-arabinosyl-2-fluoroadenine
phosphorylation of chemotherapeutically important nucleoside analogues
672894
Homo sapiens
NDP + 9-beta-D-arabinosyl-2-fluoroadenine 5'-phosphate
-
-
-
?