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Literature summary extracted from

  • Choi, K.; Noh, K.M.; Choi, J.; Park, J.; Won, H.; Kim, J.; Kim, J.; Yoon, M.
    Sulfonylurea is a non-competitive inhibitor of acetohydroxyacid synthase from Mycobacterium tuberculosis (2006), Bull. Korean Chem. Soc., 27, 1697-1700.
No PubMed abstract available

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.2.1.6 expression of the His-tagged catalytic subunit Mycobacterium tuberculosis

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.2.1.6 chlorimuron ethyl IC50: 0.009 mM, over 80% inhibition at 0.04 mM Mycobacterium tuberculosis
2.2.1.6 metsulfuron methyl IC50: 0.006 mM, over 80% inhibition at 0.04 mM Mycobacterium tuberculosis
2.2.1.6 additional information mechanism and potency of enzyme inhibition by sulfonylurea herbicides, overview Mycobacterium tuberculosis
2.2.1.6 primisulfuron methyl IC50: 0.0042 mM, over 80% inhibition at 0.04 mM Mycobacterium tuberculosis
2.2.1.6 pyrazosulfuron ethyl IC50: 87 nM, over 80% inhibition at 0.04 mM Mycobacterium tuberculosis
2.2.1.6 sulfometuron methyl IC50: 0.0048 mM, over 80% inhibition at 0.04 mM Mycobacterium tuberculosis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.2.1.6 2.76
-
 pyruvate catalytic subunit Mycobacterium tuberculosis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.2.1.6 Mg2+ a divalent metal ion is required Mycobacterium tuberculosis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.2.1.6 pyruvate Mycobacterium tuberculosis first committed step in the biosynthesis of valine and leucine 2-acetolactate + CO2
-
 ?
2.2.1.6 pyruvate Mycobacterium tuberculosis H37Rv first committed step in the biosynthesis of valine and leucine 2-acetolactate + CO2
-
 ?
2.2.1.6 pyruvate + 2-oxobutyrate Mycobacterium tuberculosis first committed step in the biosynthesis of isoleucine 2-aceto-2-hydroxybutyrate + CO2
-
 ?
2.2.1.6 pyruvate + 2-oxobutyrate Mycobacterium tuberculosis H37Rv first committed step in the biosynthesis of isoleucine 2-aceto-2-hydroxybutyrate + CO2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.2.1.6 Mycobacterium tuberculosis
-
-
-
2.2.1.6 Mycobacterium tuberculosis H37Rv
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.2.1.6 recombinant His-tagged catalytic subunit to homogeneity Mycobacterium tuberculosis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.2.1.6 pyruvate
-
 Mycobacterium tuberculosis 2-acetolactate + CO2
-
 ?
2.2.1.6 pyruvate first committed step in the biosynthesis of valine and leucine Mycobacterium tuberculosis 2-acetolactate + CO2
-
 ?
2.2.1.6 pyruvate
-
 Mycobacterium tuberculosis H37Rv 2-acetolactate + CO2
-
 ?
2.2.1.6 pyruvate first committed step in the biosynthesis of valine and leucine Mycobacterium tuberculosis H37Rv 2-acetolactate + CO2
-
 ?
2.2.1.6 pyruvate + 2-oxobutyrate
-
 Mycobacterium tuberculosis 2-aceto-2-hydroxybutyrate + CO2
-
 ?
2.2.1.6 pyruvate + 2-oxobutyrate first committed step in the biosynthesis of isoleucine Mycobacterium tuberculosis 2-aceto-2-hydroxybutyrate + CO2
-
 ?
2.2.1.6 pyruvate + 2-oxobutyrate
-
 Mycobacterium tuberculosis H37Rv 2-aceto-2-hydroxybutyrate + CO2
-
 ?
2.2.1.6 pyruvate + 2-oxobutyrate first committed step in the biosynthesis of isoleucine Mycobacterium tuberculosis H37Rv 2-aceto-2-hydroxybutyrate + CO2
-
 ?

Subunits

EC Number Subunits Comment Organism
2.2.1.6 More the enzyme consists of two diffrent types of subunits, a catalytic one and a regulatory one Mycobacterium tuberculosis

Synonyms

EC Number Synonyms Comment Organism
2.2.1.6 acetohydroxyacid synthase
-
 Mycobacterium tuberculosis
2.2.1.6 AHAS
-
 Mycobacterium tuberculosis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.2.1.6 37
-
 assay at Mycobacterium tuberculosis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.2.1.6 3.19
-
 pyruvate catalytic subunit Mycobacterium tuberculosis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.2.1.6 7.5
-
 assay at Mycobacterium tuberculosis

Cofactor

EC Number Cofactor Comment Organism Structure
2.2.1.6 FAD
-
 Mycobacterium tuberculosis
2.2.1.6 thiamine diphosphate
-
 Mycobacterium tuberculosis

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.2.1.6 additional information
-
 additional information inhibition kinetics of sulfonylurea herbicides Mycobacterium tuberculosis

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
2.2.1.6 0.000087
-
 IC50: 87 nM, over 80% inhibition at 0.04 mM Mycobacterium tuberculosis pyrazosulfuron ethyl
2.2.1.6 0.0042
-
 IC50: 0.0042 mM, over 80% inhibition at 0.04 mM Mycobacterium tuberculosis primisulfuron methyl
2.2.1.6 0.0048
-
 IC50: 0.0048 mM, over 80% inhibition at 0.04 mM Mycobacterium tuberculosis sulfometuron methyl
2.2.1.6 0.006
-
 IC50: 0.006 mM, over 80% inhibition at 0.04 mM Mycobacterium tuberculosis metsulfuron methyl
2.2.1.6 0.009
-
 IC50: 0.009 mM, over 80% inhibition at 0.04 mM Mycobacterium tuberculosis chlorimuron ethyl