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Literature summary extracted from

  • Kim, Y.G.; Yoo, J.S.; Kim, J.H.; Kim, C.M.; Oh, J.W.
    Biochemical characterization of a recombinant Japanese encephalitis virus RNA-dependent RNA polymerase (2007), BMC Mol. Biol., 8, 59.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.7.48 enzymatically active full-length recombinant JEV NS5 protein with a hexahistidine tag at the N-terminus is expressed in Escherichia coli Japanese encephalitis virus
2.7.7.48 expressed in Escherichia coli Japanese encephalitis virus

Protein Variants

EC Number Protein Variants Comment Organism
2.7.7.48 D668A inactive Japanese encephalitis virus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.7.48 K+ K+ exhibits a stimulatory effect at 25 mM, while the stimulatory effect decreases when the concentration of K+ is higher than 25 mM Japanese encephalitis virus
2.7.7.48 Mn2+ dependent on Mn2+, optimal concentration at 2.5 mM Japanese encephalitis virus
2.7.7.48 additional information no detectable level of RdRp activity is observed in the presence of 0.5 to 10 mM Mg2+ Japanese encephalitis virus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.7.48 105000
-
 SDS-PAGE Japanese encephalitis virus

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.48 Japanese encephalitis virus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.7.48 enzymatically active full-length recombinant JEV NS5 protein with a hexahistidine tag at the N-terminus Japanese encephalitis virus
2.7.7.48 SP-Sepharose column chromatography Japanese encephalitis virus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.48 nucleoside triphosphate + RNAn the NS5 protein is able to use both plus- and minus-strand 3'-untranslated regions of the JEV genome as templates in the absence of a primer, with the latter RNA being a better template. Analysis of the RNA synthesis initiation site using the 3'-end 83 nucleotides of the JEV genome as a minimal RNA template reveals that the NS5 protein specifically initiates RNA synthesis from an internal site, U81, at the two nucleotides upstream of the 3'-end of the template Japanese encephalitis virus diphosphate + RNAn+1
-
 ?
2.7.7.48 nucleoside triphosphate + RNAn poly(A) RNA template Japanese encephalitis virus diphosphate + RNAn+1
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.7.7.48 JEV NS5 nonstructural protein that carries both methyltransferase and RNA-dependent RNA polymerase (RdRp) domains Japanese encephalitis virus
2.7.7.48 JEV RdRp
-
 Japanese encephalitis virus
2.7.7.48 NS5 RdRp NS5 is a viral nonstructural protein that carries both methyltransferase and RNA-dependent RNA polymerase domains, it is a key component of the viral RNA replicase complex that presumably includes other viral nonstructural and cellular proteins Japanese encephalitis virus
2.7.7.48 RNA-dependent RNA polymerase
-
 Japanese encephalitis virus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.7.48 8
-
-
 Japanese encephalitis virus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
2.7.7.48 8
-
 rapid decrease of activity above pH 8 Japanese encephalitis virus