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Literature summary extracted from

  • Westwood, I.M.; Sim, E.
    Kinetic characterization of arylamine N-acetyltransferase from Pseudomonas aeruginosa (2007), BMC Biochem., 8, 3.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.5
-
 Pseudomonas aeruginosa

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.1.5 5-aminosalicylic acid substrate inhibition; substrate inhibition, in which the substrate binds both to the free form of the enzyme and the acetyl coenzyme A-enzyme complex in non-productive reaction pathways; substrate inhibition through 5-aminosalicylic acid in which the substrate binds both to the free form of the enzyme and the acetyl coenzyme A-enzyme complex Pseudomonas aeruginosa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.5 additional information
-
 additional information kinetics analysis, kinetic mechanism, determination of the half-life of the acetylated enzyme intermediate as 270 s, overview Pseudomonas aeruginosa
2.3.1.5 0.136
-
 acetyl-CoA concentration p-anisidine: 200 nM, reaction: acetyl-CoA + p-anisidine Pseudomonas aeruginosa
2.3.1.5 0.219
-
 5-aminosalicylic acid
-
 Pseudomonas aeruginosa
2.3.1.5 0.466
-
 acetyl-CoA concentration p-anisidine: 2 mM, reaction: acetyl-CoA + p-anisidine Pseudomonas aeruginosa
2.3.1.5 1.02
-
 acetyl-CoA
-
 Pseudomonas aeruginosa
2.3.1.5 1.02
-
 5-aminosalicylic acid
-
 Pseudomonas aeruginosa

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.5 acetyl-CoA + an arylamine Pseudomonas aeruginosa catalytic pathway, overview CoA + an N-acetylarylamine
-
 ?
2.3.1.5 additional information Pseudomonas aeruginosa the enzyme is an important drug- and carcinogen metabolising enzyme that catalyses the transfer of an acetyl group from a donor, such as acetyl coenzyme A, to an aromatic or heterocyclic amine, hydrazine, hydrazide or N-hydroxylamine acceptor substrate ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.5 Pseudomonas aeruginosa
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.5
-
 Pseudomonas aeruginosa

Reaction

EC Number Reaction Comment Organism Reaction ID
2.3.1.5 acetyl-CoA + an arylamine = CoA + an N-acetylarylamine ping pong reaction mechanism, kinetic mechanism, overview Pseudomonas aeruginosa
a

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.1.5 additional information
-
-
 Pseudomonas aeruginosa
2.3.1.5 0.567
-
 substrate aniline, p-nitrophenylacetate as an acetyl donor Pseudomonas aeruginosa
2.3.1.5 0.567
-
 substrates: aniline and p-nitrophenyl acetate Pseudomonas aeruginosa
2.3.1.5 0.602
-
 substrate isoniazid, p-nitrophenylacetate as an acetyl donor Pseudomonas aeruginosa
2.3.1.5 0.602
-
 substrates: isoniazid and p-nitrophenyl acetate Pseudomonas aeruginosa
2.3.1.5 0.629
-
 substrate aniline, AcCoA as an acetyl donor Pseudomonas aeruginosa
2.3.1.5 0.629
-
 substrates: aniline and acetyl-CoA Pseudomonas aeruginosa
2.3.1.5 0.841
-
 substrate p-aminobenzoic acid, p-nitrophenylacetate as an acetyl donor Pseudomonas aeruginosa
2.3.1.5 0.841
-
 substrates: p-aminobenzoic acid and p-nitrophenyl acetate Pseudomonas aeruginosa
2.3.1.5 1.04
-
 substrate 5-aminosalicylic acid, p-nitrophenylacetate as an acetyl donor Pseudomonas aeruginosa
2.3.1.5 1.04
-
 substrates: 5-aminosalicylic acid and p-nitrophenyl acetate Pseudomonas aeruginosa
2.3.1.5 1.47
-
 substrate 2-aminofluorene, p-nitrophenylacetate as an acetyl donor Pseudomonas aeruginosa
2.3.1.5 1.47
-
 substrates: 2-aminofluorene and p-nitrophenyl acetate Pseudomonas aeruginosa
2.3.1.5 2.22
-
 substrate p-anisidine, p-nitrophenylacetate as an acetyl donor Pseudomonas aeruginosa
2.3.1.5 2.22
-
 substrates: p-anisidine and p-nitrophenyl acetate Pseudomonas aeruginosa
2.3.1.5 2.324
-
 substrate isoniazid, AcCoA as an acetyl donor Pseudomonas aeruginosa
2.3.1.5 2.324
-
 substrates: isoniazid and acetyl-CoA Pseudomonas aeruginosa
2.3.1.5 2.99
-
 substrate hydralazine, p-nitrophenylacetate as an acetyl donor Pseudomonas aeruginosa
2.3.1.5 2.99
-
 substrates: hydralazine and p-nitrophenyl acetate Pseudomonas aeruginosa
2.3.1.5 8.2
-
 substrate p-aminobenzoic acid, AcCoA as an acetyl donor Pseudomonas aeruginosa
2.3.1.5 8.2
-
 substrates: p-aminobenzoic acid and acetyl-CoA Pseudomonas aeruginosa
2.3.1.5 13.5
-
 substrate p-anisidine, AcCoA as an acetyl donor Pseudomonas aeruginosa
2.3.1.5 13.5
-
 substrates: p-anisidine and acetyl-CoA Pseudomonas aeruginosa
2.3.1.5 29.55
-
 substrate hydralazine, AcCoA as an acetyl donor Pseudomonas aeruginosa
2.3.1.5 29.55
-
 substrates: hydralazine and acetyl-CoA Pseudomonas aeruginosa
2.3.1.5 44.71
-
 substrate 2-aminofluorene, AcCoA as an acetyl donor Pseudomonas aeruginosa
2.3.1.5 44.71
-
 substrates: 2-aminofluorene and acetyl-CoA Pseudomonas aeruginosa
2.3.1.5 73.3
-
 substrate 5-aminosalicylic acid, AcCoA as an acetyl donor Pseudomonas aeruginosa
2.3.1.5 73.3
-
 substrates: 5-aminosalicylic acid and acetyl-CoA Pseudomonas aeruginosa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.5 4-nitrophenyl acetate + 2-aminofluorene
-
 Pseudomonas aeruginosa 4-nitrophenol + N-acetyl-2-aminofluorene
-
 ?
2.3.1.5 4-nitrophenyl acetate + 4-aminobenzoic acid
-
 Pseudomonas aeruginosa 4-nitrophenol + N-acetyl-4-aminobenzoic acid
-
 ?
2.3.1.5 4-nitrophenyl acetate + 4-anisidine
-
 Pseudomonas aeruginosa 4-nitrophenol + N-acetyl-4-anisidine
-
 ?
2.3.1.5 4-nitrophenyl acetate + 5-aminosalicylic acid
-
 Pseudomonas aeruginosa 4-nitrophenol + N-acetyl-5-aminosalicylic acid
-
 ?
2.3.1.5 4-nitrophenyl acetate + aniline
-
 Pseudomonas aeruginosa 4-nitrophenol + N-acetyl-aniline
-
 ?
2.3.1.5 4-nitrophenyl acetate + hydralazine
-
 Pseudomonas aeruginosa 4-nitrophenol + N-acetyl-hydralazine
-
 ?
2.3.1.5 4-nitrophenyl acetate + isoniazid
-
 Pseudomonas aeruginosa 4-nitrophenol + N-acetyl-isoniazid
-
 ?
2.3.1.5 acetyl-CoA + 2-aminofluorene
-
 Pseudomonas aeruginosa CoA + N-acetyl-2-aminofluorene
-
 ?
2.3.1.5 acetyl-CoA + 2-aminofluorene
-
 Pseudomonas aeruginosa ?
-
 ?
2.3.1.5 acetyl-CoA + 5-aminosalicylic acid
-
 Pseudomonas aeruginosa CoA + N-acetyl-5-aminosalicylic acid
-
 ?
2.3.1.5 acetyl-CoA + 5-aminosalicylic acid
-
 Pseudomonas aeruginosa CoA + 5-acetylaminosalicylic acid
-
 ?
2.3.1.5 acetyl-CoA + an arylamine catalytic pathway, overview Pseudomonas aeruginosa CoA + an N-acetylarylamine
-
 ?
2.3.1.5 acetyl-CoA + aniline
-
 Pseudomonas aeruginosa ?
-
 ?
2.3.1.5 acetyl-CoA + aniline
-
 Pseudomonas aeruginosa CoA + N-acetyl-aniline
-
 ?
2.3.1.5 acetyl-CoA + hydralazine
-
 Pseudomonas aeruginosa ?
-
 ?
2.3.1.5 acetyl-CoA + hydralazine
-
 Pseudomonas aeruginosa CoA + N-acetyl-hydralazine
-
 ?
2.3.1.5 acetyl-CoA + isoniazid
-
 Pseudomonas aeruginosa CoA + N-acetyl-isoniazid
-
 ?
2.3.1.5 acetyl-CoA + isoniazid
-
 Pseudomonas aeruginosa ?
-
 ?
2.3.1.5 acetyl-CoA + p-aminobenzoic acid
-
 Pseudomonas aeruginosa ?
-
 ?
2.3.1.5 acetyl-CoA + p-aminobenzoic acid
-
 Pseudomonas aeruginosa CoA + N-acetyl-4-aminobenzoic acid
-
 ?
2.3.1.5 acetyl-CoA + p-anisidine
-
 Pseudomonas aeruginosa ?
-
 ?
2.3.1.5 acetyl-CoA + p-anisidine
-
 Pseudomonas aeruginosa CoA + N-acetyl-anisidine
-
 ?
2.3.1.5 additional information the enzyme is an important drug- and carcinogen metabolising enzyme that catalyses the transfer of an acetyl group from a donor, such as acetyl coenzyme A, to an aromatic or heterocyclic amine, hydrazine, hydrazide or N-hydroxylamine acceptor substrate Pseudomonas aeruginosa ?
-
 ?
2.3.1.5 additional information NAT acceptor substrate specificity, overview Pseudomonas aeruginosa ?
-
 ?
2.3.1.5 additional information enzyme follows a Ping Pong Bi Bi kinetic mechanism Pseudomonas aeruginosa ?
-
 ?
2.3.1.5 p-nitrophenyl acetate + 2-aminofluorene
-
 Pseudomonas aeruginosa p-nitrophenol + N-acetyl-2-aminofluorene
-
 ?
2.3.1.5 p-nitrophenyl acetate + 5-aminosalicylic acid
-
 Pseudomonas aeruginosa p-nitrophenol + 5-acetylaminosalicylic acid
-
 ?
2.3.1.5 p-nitrophenyl acetate + aniline
-
 Pseudomonas aeruginosa p-nitrophenol + N-acetyl-aniline
-
 ?
2.3.1.5 p-nitrophenyl acetate + hydralazine
-
 Pseudomonas aeruginosa p-nitrophenol + N-acetyl-hydralazine
-
 ?
2.3.1.5 p-nitrophenyl acetate + isoniazid
-
 Pseudomonas aeruginosa p-nitrophenol + N-acetyl-isoniazid
-
 ?
2.3.1.5 p-nitrophenyl acetate + p-aminobenzoic acid
-
 Pseudomonas aeruginosa p-nitrophenol + N-acetyl-4-aminobenzoic acid
-
 ?
2.3.1.5 p-nitrophenyl acetate + p-anisidine
-
 Pseudomonas aeruginosa p-nitrophenol + N-acetyl-4-anisidine
-
 ?
2.3.1.5 p-nitrophenylacetate + 2-aminofluorene
-
 Pseudomonas aeruginosa ?
-
 ?
2.3.1.5 p-nitrophenylacetate + 5-aminosalicylic acid
-
 Pseudomonas aeruginosa 4-nitrophenol + N-acetyl-5-aminosalicylate
-
 ?
2.3.1.5 p-nitrophenylacetate + aniline
-
 Pseudomonas aeruginosa ?
-
 ?
2.3.1.5 p-nitrophenylacetate + hydralazine
-
 Pseudomonas aeruginosa ?
-
 ?
2.3.1.5 p-nitrophenylacetate + isoniazid
-
 Pseudomonas aeruginosa ?
-
 ?
2.3.1.5 p-nitrophenylacetate + p-aminobenzoic acid
-
 Pseudomonas aeruginosa ?
-
 ?
2.3.1.5 p-nitrophenylacetate + p-anisidine
-
 Pseudomonas aeruginosa ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.3.1.5 arylamine N-acetyltransferase
-
 Pseudomonas aeruginosa
2.3.1.5 arylamine N-acetyltransferase C
-
 Pseudomonas aeruginosa
2.3.1.5 NAT
-
 Pseudomonas aeruginosa
2.3.1.5 PANAT
-
 Pseudomonas aeruginosa

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.1.5 25
-
 assay at Pseudomonas aeruginosa
2.3.1.5 25
-
 activity assay Pseudomonas aeruginosa

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.3.1.5 434
-
 5-aminosalicylic acid
-
 Pseudomonas aeruginosa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.5 8
-
 assay at Pseudomonas aeruginosa
2.3.1.5 8
-
 activity assay Pseudomonas aeruginosa

Cofactor

EC Number Cofactor Comment Organism Structure
2.3.1.5 acetyl-CoA
-
 Pseudomonas aeruginosa