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Literature summary extracted from

  • Weselake, R.J.; Madhavji, M.; Szarka, S.J.; Patterson, N.A.; Wiehler, W.B.; Nykiforuk, C.L.; Burton, T.L.; Boora, P.S.; Mosimann, S.C.; Foroud, N.A.; Thibault, B.J.; Moloney, M.M.; Laroche, A.; Furukawa-Stoffer, T.L.
    Acyl-CoA-binding and self-associating properties of a recombinant 13.3 kDa N-terminal fragment of diacylglycerol acyltransferase-1 from oilseed rape (2006), BMC Biochem., 7, 24.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.20 expression of the poly-His-tagged N-terminal fragment of the enzyme, i.e. BnDGAT1(1–116)His6, in Escherichia coli strain BL21(DE3), BnDGAT1(1–116)His6 fails to react with protein in microsomal vesicles following treatment with proteinase K, and thus might be localized to the cytosolic side of the ER Brassica napus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.20 additional information
-
 additional information acyl-CoA substrate binding kinetics of the recombinant N-terminal fragment, overview Brassica napus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.3.1.20 microsome
-
 Brassica napus
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.1.20 13278
-
 x * 13278, recombinant poly-His-tagged N-terminal enzyme fragment comprising residues 1-116, sequence calculation Brassica napus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.20 sn-1,2-diacylglycerol + acyl-CoA Brassica napus
-
 triacylglycerol + CoA
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.20 Brassica napus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.20 recombinant poly-His-tagged N-terminal fragment of the enzyme, i.e. BnDGAT1(1–116)His6, from Escherichia coli strain BL21(DE3) Brassica napus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.20 1,2-diacyl-sn-glycerol + erucoyl-CoA
-
 Brassica napus 1,2-diacyl-3-erucoyl-sn-glycerol + CoA
-
 ?
2.3.1.20 1,2-diacyl-sn-glycerol + oleoyl-CoA
-
 Brassica napus 1,2-diacyl-3-oleoyl-sn-glycerol + CoA
-
 ?
2.3.1.20 sn-1,2-diacylglycerol + acyl-CoA
-
 Brassica napus triacylglycerol + CoA
-
 ?
2.3.1.20 sn-1,2-diacylglycerol + erucoyl-CoA i.e. a 22:1cisDELTA13-CoA Brassica napus triacylglycerol + CoA
-
 ?

Subunits

EC Number Subunits Comment Organism
2.3.1.20 ? x * 13278, recombinant poly-His-tagged N-terminal enzyme fragment comprising residues 1-116, sequence calculation Brassica napus
2.3.1.20 More analysis of self-associating properties of BnDGAT1(1–116)His6 in an gel filtration approach, the recombinant N-terminal fragment associates to form tetramers Brassica napus

Synonyms

EC Number Synonyms Comment Organism
2.3.1.20 DGAT1
-
 Brassica napus
2.3.1.20 diacylglycerol acyltransferase-1
-
 Brassica napus