EC Number | Cloned (Comment) | Organism |
---|---|---|
1.3.7.7 | expressed in Escherichia coli BL21(DE3)Lys cells | Auxenochlorella protothecoides |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
1.3.7.7 | chloroplast | - |
Auxenochlorella protothecoides | 9507 | - |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.3.7.7 | 38000 | - |
1 * 38000 + 1 * 49000 + 1 * 58000, SDS-PAGE | Auxenochlorella protothecoides |
1.3.7.7 | 49000 | - |
1 * 38000 + 1 * 49000 + 1 * 58000, SDS-PAGE | Auxenochlorella protothecoides |
1.3.7.7 | 58000 | - |
1 * 38000 + 1 * 49000 + 1 * 58000, SDS-PAGE | Auxenochlorella protothecoides |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.3.7.7 | chlorophyllide a + reduced ferredoxin + ATP | Auxenochlorella protothecoides | - |
protochlorophyllide + oxidized ferredoxin + ADP + phosphate | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.3.1.33 | Auxenochlorella protothecoides | - |
enzyme components protein L, protein N and protein B are all transcribed constitutively independent of illumination and presence of glucose. Steady-state amounts of proteins in the light-grown cells are two- to threefold greater than in dark-grown cells. Approximately the same amount of protein is present in cultures grown mixtrophically or heterotrophically both containing glucose. Much less protein is present in photoautotrophic cells. Therefore light-independent enzyme activity depends on post-transcriptional and post-translational regulation and switches on both in light and dark | - |
1.3.1.33 | Auxenochlorella protothecoides CS-41 | - |
enzyme components protein L, protein N and protein B are all transcribed constitutively independent of illumination and presence of glucose. Steady-state amounts of proteins in the light-grown cells are two- to threefold greater than in dark-grown cells. Approximately the same amount of protein is present in cultures grown mixtrophically or heterotrophically both containing glucose. Much less protein is present in photoautotrophic cells. Therefore light-independent enzyme activity depends on post-transcriptional and post-translational regulation and switches on both in light and dark | - |
1.3.7.7 | Auxenochlorella protothecoides | Q6VQA8 and Q6VQA9 and Q7YKW4 | subunit B, subunit L, and subunit N; strain CS-41, also named Auxenochlorella protothecoides strain CS-41 | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.3.7.7 | chlorophyllide a + reduced ferredoxin + ATP | - |
Auxenochlorella protothecoides | protochlorophyllide + oxidized ferredoxin + ADP + phosphate | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.3.7.7 | heterodimer | 1 * 38000 + 1 * 49000 + 1 * 58000, SDS-PAGE | Auxenochlorella protothecoides |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.3.1.33 | light-independent protochlorophyllide oxidoreductase | - |
Auxenochlorella protothecoides |
1.3.1.33 | LIPOR | - |
Auxenochlorella protothecoides |
1.3.7.7 | ChlB | subunit B | Auxenochlorella protothecoides |
1.3.7.7 | ChlL | subunit L | Auxenochlorella protothecoides |
1.3.7.7 | ChlN | subunit N | Auxenochlorella protothecoides |
1.3.7.7 | light-independent protochlorophyllide oxidoreductase | - |
Auxenochlorella protothecoides |
1.3.7.7 | light-independent protochlorophyllide reductase | - |
Auxenochlorella protothecoides |
1.3.7.7 | LIPOR | - |
Auxenochlorella protothecoides |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.3.7.7 | 4Fe-4S-center | the enzyme contains an Fe-S cluster as redox center in all subunits | Auxenochlorella protothecoides | |
1.3.7.7 | ATP | subunit L contains the ATP-binding motif | Auxenochlorella protothecoides |
EC Number | Organism | Comment | Expression |
---|---|---|---|
1.3.7.7 | Auxenochlorella protothecoides | the level of theLIPOR subunit L mRNA of Chlorella protothecoides strain CS-41 is much higher in the light than in the dark | up |
EC Number | General Information | Comment | Organism |
---|---|---|---|
1.3.7.7 | physiological function | light-independent protochlorophyllide reductase is required for protochlorophyllide reduction in the dark | Auxenochlorella protothecoides |