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Literature summary extracted from

  • Han, S.Q.; Ouyang, P.K.; Wei, P.; Hu, Y.
    Enzymatic synthesis of (R)-cyanohydrins by a novel (R)-oxynitrilase from Vicia sativa L (2006), Biotechnol. Lett., 28, 1909-1912.
    View publication on PubMed

Organism

EC Number Organism UniProt Comment Textmining
4.1.2.B6 Vicia sativa
-
-
-

Reaction

EC Number Reaction Comment Organism Reaction ID
4.1.2.B6 (R)-mandelonitrile = cyanide + benzaldehyde no activity with aliphatic substrates Vicia sativa
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
4.1.2.B6 seed defatted meal Vicia sativa
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.2.B6 cyanide + 1,3-benzodioxole-5-carbaldehyde
-
 Vicia sativa (2S)-1,3-benzodioxol-5-yl(hydroxy)ethanenitrile 93% yield, 88% enantiomeric excess ?
4.1.2.B6 cyanide + 4-fluorobenzaldehyde
-
 Vicia sativa (2R)-(4-fluorophenyl)(hydroxy)ethanenitrile 97% yield, 91% enantiomeric excess ?
4.1.2.B6 cyanide + 4-methoxybenzaldehyde
-
 Vicia sativa (2R)-hydroxy(4-methoxyphenyl)ethanenitrile 52% yield, 94% enantiomeric excess ?
4.1.2.B6 cyanide + benzaldehyde
-
 Vicia sativa (R)-mandelonitrile 100% yield, 99% enantiomeric excess ?
4.1.2.B6 cyanide + furan-2-carbaldehyde
-
 Vicia sativa (2S)-furan-2-yl(hydroxy)ethanenitrile 96% yield, 97% enantiomeric excess. (S)-configuration is due to the Cahn-Ingold-Prelog rules ?
4.1.2.B6 cyanide + pyridine-3-carbaldehyde
-
 Vicia sativa (2R)-hydroxy(pyridin-3-yl)ethanenitrile 71% yield, 3% enantiomeric excess ?
4.1.2.B6 additional information no activity with heptanal Vicia sativa ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
4.1.2.B6 (R)-Oxynitrilase
-
 Vicia sativa