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Literature summary extracted from

  • Loncaric, C.; Ward, A.F.; Walker, K.D.
    Expression of an acetyl-CoA synthase and a CoA-transferase in Escherichia coli to produce modified taxanes in vivo (2007), Biotechnol. J., 2, 266-274.
    View publication on PubMed

Application

EC Number Application Comment Organism
2.3.1.167 biotechnology optimizing the semi-biosynthetic method in bacteria potentially provides a practical means of developing commercial-scale production of baccatin III analogs, which serve as key intermediates in the semi-synthesis of second-generation taxols Taxus sp.
2.3.1.167 synthesis bioengineered Escherichia coli cells synthesize nonnatural 10-deacetyl-10-acylbaccatin III derivatives as potential precursors of second-generation Taxol derivatives using the enzyme in concert with acyl-CoA transferases, determination of optimal alkanoic acid concentration for in vivo production of baccatin III analogues, overview Taxus sp.

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.167 gene dbat, functional expression in Escherichia coli strain JM109, co-expression with acetyl-CoA synthase, gene acs, EC 6.2.1.1, or with acetoacetate:acetyl-CoA CoA-transferase, genes atoAD of the ato operon, EC 2.8.3.8, expressed from and regulated by genes encoded on the bacterial chromosome, overview Taxus sp.
2.3.1.167 into the vector pCWori+ for expression in Escherichia coli JM109 and BL21DE3 cells Taxus sp.
6.2.1.1 10-deacetylbaccatin III 10beta-O-acetyltransferase (DBAT) from Taxus can catalyze the transfer of acetyl, propionyl or n-butyryl from CoA to the C10-hydroxyl of 10-deacetylbaccatin III. Escherichia coli JM109 are transformed to recombinantly express dbat, and this enzyme function is coupled to that of acetyl-CoA synthase expressed from and regulated by genes encoded on the bacterial chromosome Taxus sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.167 acetyl-CoA + 10-deacetylbaccatin III Taxus sp.
-
 CoA + baccatin III
-
 ?
2.3.1.167 butyryl-CoA + 10-deacetylbaccatin III Taxus sp.
-
 CoA + 10-deacetyl-10-butyrylbaccatin III
-
 ?
2.3.1.167 propionyl-CoA + 10-deacetylbaccatin III Taxus sp.
-
 CoA + 10-deacetyl-10-propionylbaccatin III
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.167 Taxus sp.
-
-
-
2.3.1.167 Taxus sp.
-
 Taxus
-
6.2.1.1 Taxus sp.
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.167 acetyl-CoA + 10-deacetylbaccatin III
-
 Taxus sp. CoA + baccatin III
-
 ?
2.3.1.167 butyryl-CoA + 10-deacetylbaccatin III
-
 Taxus sp. CoA + 10-deacetyl-10-butyrylbaccatin III
-
 ?
2.3.1.167 additional information DBAT catalyzes the transfer of acetyl, propionyl or n-butyryl from CoA to the C10-hydroxyl of 10-deacetylbaccatin III Taxus sp. ?
-
 ?
2.3.1.167 n-butyryl-CoA + 10-deacetylbaccatin III
-
 Taxus sp. CoA + 10-deacetyl-10-butyrylbaccatin III
-
 ?
2.3.1.167 propionyl-CoA + 10-deacetylbaccatin III
-
 Taxus sp. CoA + 10-deacetyl-10-propionylbaccatin III
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.3.1.167 10-deacetylbaccatin III 10-O-acetyltransferase
-
 Taxus sp.
2.3.1.167 10-deacetylbaccatin III 10beta-O-acetyltransferase
-
 Taxus sp.
2.3.1.167 DBAT
-
 Taxus sp.

Cofactor

EC Number Cofactor Comment Organism Structure
2.3.1.167 acetyl-CoA
-
 Taxus sp.