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Literature summary extracted from

  • Jennewein, S.; Park, H.; DeJong, J.M.; Long, R.M.; Bollon, A.P.; Croteau, R.B.
    Coexpression in yeast of Taxus cytochrome P450 reductase with cytochrome P450 oxygenases involved in taxol biosynthesis (2005), Biotechnol. Bioeng., 89, 588-598.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.14.105 isolation and cloning of a single-copy gene, DNA and amino acid sequence determination and analysis, expression in insect cells, functional co-expression of full-length and truncated enzyme with taxoid 13beta-hydroxylase or with the organisms' taxoid 10beta-hydroxylase in Saccharomyces cerevisiae strain YPH499, subcloning in Escherichia coli Taxus cuspidata
1.14.14.105 isolation and cloning of a single-copy gene, DNA and amino acid sequence determination and analysis, functional co-expression with the NADPH:cytochrome P450 reductase, also exhibiting taxoid 10beta-hydroxylase activity, in Saccharomyces cerevisiae strain YPH499, subcloning in Escherichia coli Taxus cuspidata
1.14.14.106 functional co-expression with taxoid 10beta-hydroxylase in Saccharomyces cerevisiae strain YPH499, subcloning in Escherichia coli Taxus cuspidata

Protein Variants

EC Number Protein Variants Comment Organism
1.14.14.105 additional information construction of a truncated enzyme version Taxus cuspidata

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.14.14.105 endoplasmic reticulum
-
 Taxus cuspidata 5783
-
1.14.14.105 microsome
-
 Taxus cuspidata
-
-
1.14.14.106 endoplasmic reticulum
-
 Taxus cuspidata 5783
-
1.14.14.106 microsome
-
 Taxus cuspidata
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.14.14.105 Fe2+ heme iron Taxus cuspidata
1.14.14.106 Fe2+ heme iron Taxus cuspidata

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.14.105 taxadien-5alpha-yl acetate + [reduced NADPH-hemoprotein reductase] + O2 Taxus cuspidata the enzyme is involved in the taxol biosynthetic pathway, overview 10beta-hydroxytaxa-4(20),11-dien-5alpha-yl acetate + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
1.14.14.106 taxadien-5alpha-yl acetate + [reduced NADPH-hemoprotein reductase] + O2 Taxus cuspidata the enzyme is involved in the taxol biosynthetic pathway, overview taxa-4(20),11(12)-dien-5alpha-yl-acetyl-13alpha-ol + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.14.105 Taxus cuspidata Q9AXM6
-
-
1.14.14.106 Taxus cuspidata
-
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.14.14.105 additional information
-
-
 Taxus cuspidata
1.14.14.106 additional information
-
-
 Taxus cuspidata

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.14.105 cytochrome c + FAD + FMN + O2 rat cytochrome b5 Taxus cuspidata ?
-
 ?
1.14.14.105 taxadien-5alpha-yl acetate + [reduced NADPH-hemoprotein reductase] + O2
-
 Taxus cuspidata 10beta-hydroxytaxa-4(20),11-dien-5alpha-yl acetate + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
1.14.14.105 taxadien-5alpha-yl acetate + [reduced NADPH-hemoprotein reductase] + O2 the enzyme is involved in the taxol biosynthetic pathway, overview Taxus cuspidata 10beta-hydroxytaxa-4(20),11-dien-5alpha-yl acetate + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
1.14.14.106 taxadien-5alpha-yl acetate + [reduced NADPH-hemoprotein reductase] + O2 the enzyme is involved in the taxol biosynthetic pathway, overview Taxus cuspidata taxa-4(20),11(12)-dien-5alpha-yl-acetyl-13alpha-ol + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
1.14.14.106 taxadien-5alpha-yl acetate + [reduced NADPH-hemoprotein reductase] + O2
-
 Taxus cuspidata taxa-4(20),11-dien-5alpha-yl-acetyl-13alpha-ol + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.14.14.105 cytochrome P450 reductase
-
 Taxus cuspidata
1.14.14.105 NADPH:cytochrome P450 reductase
-
 Taxus cuspidata
1.14.14.105 taxoid 10beta-hydroxylase
-
 Taxus cuspidata
1.14.14.106 cytochrome P450 taxoid 13alpha-hydroxylase
-
 Taxus cuspidata
1.14.14.106 taxoid 13alpha-hydroxylase
-
 Taxus cuspidata

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.14.14.105 7.7
-
 assay at Taxus cuspidata
1.14.14.106 7.7
-
 assay at Taxus cuspidata

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.14.105 cytochrome P-450
-
 Taxus cuspidata
1.14.14.105 FAD
-
 Taxus cuspidata
1.14.14.105 FMN
-
 Taxus cuspidata
1.14.14.106 cytochrome P-450
-
 Taxus cuspidata