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Literature summary extracted from

  • Hirano, S.; Morikawa, M.; Takano, K.; Imanaka, T.; Kanaya, S.
    Gentisate 1,2-dioxygenase from Xanthobacter polyaromaticivorans 127W (2007), Biosci. Biotechnol. Biochem., 71, 192-199.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.13.11.4 expressed in Escherichia coli Xanthobacter polyaromaticivorans

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.13.11.4 1-hydroxy 2 naphthoate 0.33 mM, 19% of wild type enzyme activity remaining Xanthobacter polyaromaticivorans
1.13.11.4 Fe 2 mM, complete inactivation Xanthobacter polyaromaticivorans
1.13.11.4 salicylate 0.33 mM, 38% of wild type enzyme activity remaining Xanthobacter polyaromaticivorans

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.13.11.4 0.0186
-
 2,5-Dihydroxybenzoate
-
 Xanthobacter polyaromaticivorans
1.13.11.4 0.0209
-
 1-hydroxy 2 naphthoate
-
 Xanthobacter polyaromaticivorans

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.13.11.4 Iron ferrous ion specifically functions in the formation of the active tetramer structure, the enzyme contains 0.98 mol iron per mol subunit Xanthobacter polyaromaticivorans

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.13.11.4 36500
-
 4 * 36500, gel filtration Xanthobacter polyaromaticivorans
1.13.11.4 39300
-
 calculated from amino acid sequence Xanthobacter polyaromaticivorans
1.13.11.4 40000
-
 SDS-PAGE Xanthobacter polyaromaticivorans
1.13.11.4 146000
-
 gel filtration Xanthobacter polyaromaticivorans

Organism

EC Number Organism UniProt Comment Textmining
1.13.11.4 Xanthobacter polyaromaticivorans Q75W71
-
-
1.13.11.4 Xanthobacter polyaromaticivorans 127W Q75W71
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.13.11.4 HiTrapQ column chromatography Xanthobacter polyaromaticivorans

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.13.11.4 additional information
-
 the specific activity of the purified recombinant enzyme is 102 units/mg at pH 7.5 Xanthobacter polyaromaticivorans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.11.4 1,4-dihydroxy-2-naphthoate + O2
-
 Xanthobacter polyaromaticivorans ?
-
 ?
1.13.11.4 1,4-dihydroxy-2-naphthoate + O2
-
 Xanthobacter polyaromaticivorans 127W ?
-
 ?
1.13.11.4 1-hydroxy-2-naphthoate + O2 weak activity Xanthobacter polyaromaticivorans ?
-
 ?
1.13.11.4 1-hydroxy-2-naphthoate + O2 weak activity Xanthobacter polyaromaticivorans 127W ?
-
 ?
1.13.11.4 2,5-dihydroxybenzoate + O2
-
 Xanthobacter polyaromaticivorans maleylpyruvate
-
 ?
1.13.11.4 2,5-dihydroxybenzoate + O2
-
 Xanthobacter polyaromaticivorans 127W maleylpyruvate
-
 ?
1.13.11.4 5-aminosalicylate + O2 partly preferred substrate Xanthobacter polyaromaticivorans ?
-
 ?
1.13.11.4 5-aminosalicylate + O2 partly preferred substrate Xanthobacter polyaromaticivorans 127W ?
-
 ?
1.13.11.4 additional information no activity against catechol, benzoate, 5-methylsalicylate, 5-chlorosalicylate, and homogentisate Xanthobacter polyaromaticivorans ?
-
 ?
1.13.11.4 additional information no activity against catechol, benzoate, 5-methylsalicylate, 5-chlorosalicylate, and homogentisate Xanthobacter polyaromaticivorans 127W ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.13.11.4 homotetramer 4 * 36500, gel filtration Xanthobacter polyaromaticivorans

Synonyms

EC Number Synonyms Comment Organism
1.13.11.4 GDO
-
 Xanthobacter polyaromaticivorans
1.13.11.4 gentisate dioxygenase
-
 Xanthobacter polyaromaticivorans

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.13.11.4 50
-
 the enzyme loses almost all its activity after incubation at 50°C for 15 min Xanthobacter polyaromaticivorans

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.13.11.4 0.565
-
 1-hydroxy 2 naphthoate
-
 Xanthobacter polyaromaticivorans
1.13.11.4 5.5
-
 2,5-Dihydroxybenzoate
-
 Xanthobacter polyaromaticivorans
1.13.11.4 6.08
-
 1-hydroxy 2 naphthoate
-
 Xanthobacter polyaromaticivorans

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.13.11.4 6.5
-
 GDO loses its activity after being kept in buffer below pH 6.5 for 12 h in the absence of ferrous iron and cysteine Xanthobacter polyaromaticivorans
1.13.11.4 8
-
 GDO loses its activity after being kept in buffer above pH 8.0 for 12 h in the absence of ferrous iron and cysteine Xanthobacter polyaromaticivorans

pI Value

EC Number Organism Comment pI Value Maximum pI Value
1.13.11.4 Xanthobacter polyaromaticivorans calculated from amino acid sequence
-
 5.4