Any feedback?
Literature summary extracted from
- TreX from Sulfolobus solfataricus ATCC 35092 displays isoamylase and 4-alpha-glucanotransferase activities (2007), Biosci. Biotechnol. Biochem., 71, 1348-1352.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 2.4.1.25 | expressed in Escherichia coli MC1061 as a 6*His tagged protein | Saccharolobus solfataricus |
| 3.2.1.68 | expression of in Escherichia coli | Saccharolobus solfataricus |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 2.4.1.25 | 83000 | - |
x * 83000, SDS-PAGE | Saccharolobus solfataricus |
| 3.2.1.68 | 830000 | - |
SDS-PAGE | Saccharolobus solfataricus |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 2.4.1.25 | Saccharolobus solfataricus | - |
ATCC 35092 | - |
| 3.2.1.68 | Saccharolobus solfataricus | - |
ATCC 35092 | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 2.4.1.25 | - |
Saccharolobus solfataricus |
| 3.2.1.68 | recombinant protein by His tag column chromatography | Saccharolobus solfataricus |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.2.1.68 | amylopectin + H2O | - |
Saccharolobus solfataricus | linear maltooligosaccharides | - |
? |  |
| 3.2.1.68 | glycogen + H2O | - |
Saccharolobus solfataricus | linear maltooligosaccharides | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 2.4.1.25 | ? | x * 83000, SDS-PAGE | Saccharolobus solfataricus |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 2.4.1.25 | TreX | enzyme displays isoamylase and 4-alpha-glucanotransferase activity | Saccharolobus solfataricus |
| 3.2.1.68 | glycogen alpha-1,6-glucanohydrolase | - |
Saccharolobus solfataricus |
| 3.2.1.68 | isoamylase | - |
Saccharolobus solfataricus |
| 3.2.1.68 | TreX | trehalose gene cluster has isoamylase activities | Saccharolobus solfataricus |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 2.4.1.25 | 75 | - |
- |
Saccharolobus solfataricus |
| 3.2.1.68 | 75 | - |
- |
Saccharolobus solfataricus |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 2.4.1.25 | 5.5 | - |
- |
Saccharolobus solfataricus |
| 3.2.1.68 | 5.5 | - |
- |
Saccharolobus solfataricus |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
