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Literature summary extracted from

  • Nishimura, M.; Ishiyama, D.; Davies, J.
    Molecular cloning of Streptomyces genes encoding vanillate demethylase (2006), Biosci. Biotechnol. Biochem., 70, 2316-2319.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.13.82 genes vanA and vanB, DNA and amino acid sequence determination and analysis, genetic organization, sequence comparison, functional coexpression of genes vanA and vanB in Streptomyces lividans strain 1326 from the high-copy plasmid pIJ702, selection on veratric acid Streptomyces sp.

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.14.13.82 Fe2+ binding sequence motif of iron-sulfur cluster, overview Streptomyces sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.13.82 additional information Streptomyces sp. the enzyme forms a vanillate demethylase complex of VanA, a terminal oxygenase subunit, with VanB, a ferredoxin-like subunit, performing demethylation of vanillic acid and veratric acid ?
-
?
1.14.13.82 vanillic acid + NADH + H+ + O2 Streptomyces sp.
-
protocatechuic acid + NAD+ + H2O + formaldehyde procatechuic acid is 3,4-dihydroxybenzoate ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.13.82 Streptomyces sp.
-
genes vanA and vanB
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.13.82 additional information the enzyme forms a vanillate demethylase complex of VanA, a terminal oxygenase subunit, with VanB, a ferredoxin-like subunit, performing demethylation of vanillic acid and veratric acid Streptomyces sp. ?
-
?
1.14.13.82 vanillic acid + NADH + H+ + O2
-
Streptomyces sp. protocatechuic acid + NAD+ + H2O + formaldehyde procatechuic acid is 3,4-dihydroxybenzoate ?

Synonyms

EC Number Synonyms Comment Organism
1.14.13.82 vanAB
-
Streptomyces sp.
1.14.13.82 vanillate demethylase
-
Streptomyces sp.

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.13.82 NADH binding sequence motif, overview Streptomyces sp.