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Literature summary extracted from

  • Kim, J.H.; Kim, B.G.; Park, Y.; Ko, J.H.; Lim, C.E.; Lim, J.; Lim, Y.; Ahn, J.
    Characterization of flavonoid 7-O-glucosyltransferase from Arabidopsis thaliana (2006), Biosci. Biotechnol. Biochem., 70, 1471-1477.
    View publication on PubMed

Application

EC Number Application Comment Organism
2.4.1.81 synthesis biotransformation of eriodictyol using Escherichi coli expressing AtGT-2 Arabidopsis thaliana

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.81 expression in Escherichia coli as a glutathione S-transferase fusion protein Arabidopsis thaliana
2.4.1.170 expressed in Escherichia coli Arabidopsis thaliana

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.81 0.0018
-
 eriodictyol pH 7.0 Arabidopsis thaliana
2.4.1.81 0.0263
-
 kaempferol pH 7.0 Arabidopsis thaliana
2.4.1.81 0.0353
-
 quercetin pH 7.0 Arabidopsis thaliana
2.4.1.170 0.018
-
 eriodictyol
-
 Arabidopsis thaliana
2.4.1.170 0.0263
-
 kaempferol
-
 Arabidopsis thaliana
2.4.1.170 0.0353
-
 quercetin
-
 Arabidopsis thaliana

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.1.81 54800
-
 x * 54800, calculated from sequence Arabidopsis thaliana

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.81 Arabidopsis thaliana Q8VZE9
-
-
2.4.1.170 Arabidopsis thaliana Q8VZE9
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.170
-
 Arabidopsis thaliana

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.1.81 flower high expression Arabidopsis thaliana
-
2.4.1.81 leaf high expression Arabidopsis thaliana
-
2.4.1.81 root
-
 Arabidopsis thaliana
-
2.4.1.81 stem
-
 Arabidopsis thaliana
-
2.4.1.170 flower AtGT-2 is expressed in this tissue, higher in the flowers and leaves than in roots and stems Arabidopsis thaliana
-
2.4.1.170 leaf AtGT-2 is expressed in this tissue, higher in the flowers and leaves than in roots and stems Arabidopsis thaliana
-
2.4.1.170 root AtGT-2 is expressed in this tissue, higher in the flowers and leaves than in roots and stems Arabidopsis thaliana
-
2.4.1.170 stem AtGT-2 is expressed in this tissue, higher in the flowers and leaves than in roots and stems Arabidopsis thaliana
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.1.170 additional information
-
 Vmax for eriodictyol is 333.3 pkat/mg, 333.3 pkat/mg for kaempferol and 250 pkat/mg for quercetin. Assay at pH 7.0 and 37°C Arabidopsis thaliana

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.81 UDP-alpha-D-glucose + apigenin 29.6% of the activity with eriodictyol Arabidopsis thaliana UDP + apigenin 7-O-beta-D-glucoside
-
 ?
2.4.1.81 UDP-alpha-D-glucose + kaempferol 78.9% of the activity with eriodictyol Arabidopsis thaliana UDP + kaempferol 7-O-beta-D-glucoside
-
 ?
2.4.1.81 UDP-alpha-D-glucose + quercetin 64.1% of the activity with eriodictyol Arabidopsis thaliana UDP + quercetin 7-O-beta-D-glucoside
-
 ?
2.4.1.81 UDP-glucose + eriodictyol
-
 Arabidopsis thaliana UDP + 7-O-beta-D-glucosyl-eriodictyol
-
 ?
2.4.1.81 UDP-glucose + luteolin 92.1% of the activity with eriodictyol Arabidopsis thaliana UDP + 7-O-beta-D-glucosyl-luteolin
-
 ?
2.4.1.81 UDP-glucose + naringenin 71.6% of the activity with eriodictyol Arabidopsis thaliana UDP + 7-O-beta-D-glucosyl-naringenin
-
 ?
2.4.1.170 additional information caffeic acid and esculetin do not react with the enzyme. AtGT-2 prefers flavonene to flavone or flavonol, both of which contain a double bond in the C ring. The relative conversion rate is 100% for eriodictyol, 71.6% for naringenin, 29.6% for apigenin, 92.1% for luteolin, 78.9% for kaempferol and 64.1% for quercetin. Arabidopsis thaliana ?
-
 ?
2.4.1.170 UDP-glucose + eriodictyol
-
 Arabidopsis thaliana UDP + eriodictyol 7-O-beta-D-glucoside
-
 ?
2.4.1.170 UDP-glucose + kaempferol
-
 Arabidopsis thaliana UDP + kaempferol 7-O-beta-D-glucoside
-
 ?
2.4.1.170 UDP-glucose + quercetin
-
 Arabidopsis thaliana UDP + quercetin 7-O-beta-D-glucoside
-
 ?

Subunits

EC Number Subunits Comment Organism
2.4.1.81 ? x * 54800, calculated from sequence Arabidopsis thaliana

Synonyms

EC Number Synonyms Comment Organism
2.4.1.81 AtGT-2
-
 Arabidopsis thaliana
2.4.1.170 AtGT-2 AtGT-2 encodes a flavonoid 7-O-glucosyltransferase Arabidopsis thaliana

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.4.1.170 additional information
-
 additional information Kcat/Km for eriodictyol is 2.66 microM-1/mg-1, 1.32 microM-1/mg-1 for kaempferol and 250 microM-1/mg-1 for quercetin. Assay at pH 7.0 and 37°C Arabidopsis thaliana