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Literature summary extracted from

  • Sano, S.; Tao, S.; Endo, Y.; Inaba, T.; Hossain, M.A.; Miyake, C.; Matsuo, M.; Aoki, H.; Asada, K.; Saito, K.
    Purification and cDNA cloning of chloroplastic monodehydroascorbate reductase from spinach (2005), Biosci. Biotechnol. Biochem., 69, 762-772.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.6.5.4 expressed in Escherichia coli Spinacia oleracea

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.6.5.4 dicoumarol
-
 Spinacia oleracea
1.6.5.4 iodoacetate 5 mM, 58% inhibition Spinacia oleracea
1.6.5.4 additional information not inhibited by 5 mM N-ethylmaleimide Spinacia oleracea
1.6.5.4 p-chloromercuribenzoate 0.1 mM, 90% inhibition Spinacia oleracea

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.6.5.4 0.0063
-
 NADH at 0.003 mM monodehydroascorbate Spinacia oleracea

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.6.5.4 chloroplast
-
 Spinacia oleracea 9507
-
1.6.5.4 cytosol
-
 Spinacia oleracea 5829
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.6.5.4 50000
-
 gel filtration, chloroplastic enzyme Spinacia oleracea
1.6.5.4 53000
-
 SDS-PAGE, cytosolic enzyme Spinacia oleracea

Organism

EC Number Organism UniProt Comment Textmining
1.6.5.4 Spinacia oleracea
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.6.5.4 Butyl-Toyopearl column chromatography, HiTrap Q column chromatography and Blue Sepharose 6FF column chromatography Spinacia oleracea

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.6.5.4 leaf
-
 Spinacia oleracea
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.6.5.4 0.056
-
 cell extract, wild type enzyme Spinacia oleracea
1.6.5.4 13.1
-
 cell extract, recombinant enzyme Spinacia oleracea
1.6.5.4 24
-
 after 429fold purification, wild type enzyme Spinacia oleracea
1.6.5.4 100
-
 after 7.6fold purification, recombinant enzyme Spinacia oleracea

Storage Stability

EC Number Storage Stability Organism
1.6.5.4 -80°C, buffer containing 10 mM potassium phosphate at pH 6.5 10 mM 2-mercaptoethanol and 0.1 mM EDTA, several months, no loss of activity Spinacia oleracea

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.6.5.4 2 ferricyanide + NADPH
-
 Spinacia oleracea 2 ferrocyanide + NADP+ + H+
-
 ?
1.6.5.4 NADH + 2,6-dichlorophenol indophenol
-
 Spinacia oleracea NAD+ + ?
-
 ?
1.6.5.4 NADH + monodehydroascorbate
-
 Spinacia oleracea NAD+ + ascorbate
-
 ?
1.6.5.4 NADH + monodehydroisoascorbate
-
 Spinacia oleracea NAD+ + ?
-
 ?
1.6.5.4 NADH + p-benzoquinone
-
 Spinacia oleracea NAD+ + ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.6.5.4 MDA reductase
-
 Spinacia oleracea
1.6.5.4 monodehydroascorbate radical reductase
-
 Spinacia oleracea

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.6.5.4 6.5 9
-
 Spinacia oleracea

Cofactor

EC Number Cofactor Comment Organism Structure
1.6.5.4 FAD
-
 Spinacia oleracea
1.6.5.4 FMN
-
 Spinacia oleracea
1.6.5.4 NADH
-
 Spinacia oleracea

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.6.5.4 0.095
-
 dicoumarol
-
 Spinacia oleracea